Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05620 and PGA1_c05630 are separated by 25 nucleotides PGA1_c05630 and PGA1_c05640 are separated by 37 nucleotides PGA1_c05640 and PGA1_c05650 are separated by 155 nucleotides
PGA1_c05620: PGA1_c05620 - putative 7-cyano-7-deazaguanosine biosynthesis protein QueE, at 575,246 to 576,043
_c05620
PGA1_c05630: PGA1_c05630 - NADPH-dependent 7-cyano-7-deazaguanine reductase QueF, at 576,069 to 576,533
_c05630
PGA1_c05640: PGA1_c05640 - hypothetical protein, at 576,571 to 577,242
_c05640
PGA1_c05650: PGA1_c05650 - tRNA pseudouridine synthase A, at 577,398 to 578,171
_c05650
Position (kb) 576
577
578 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 575.617 kb on + strand, within PGA1_c05620 at 575.617 kb on + strand, within PGA1_c05620 at 575.617 kb on + strand, within PGA1_c05620 at 575.651 kb on + strand, within PGA1_c05620 at 575.671 kb on + strand, within PGA1_c05620 at 575.684 kb on - strand, within PGA1_c05620 at 575.702 kb on - strand, within PGA1_c05620 at 575.761 kb on + strand, within PGA1_c05620 at 575.761 kb on + strand, within PGA1_c05620 at 575.779 kb on - strand, within PGA1_c05620 at 575.822 kb on + strand, within PGA1_c05620 at 575.884 kb on + strand, within PGA1_c05620 at 575.890 kb on + strand, within PGA1_c05620 at 575.979 kb on + strand at 575.987 kb on - strand at 576.016 kb on + strand at 576.070 kb on + strand at 576.101 kb on + strand at 576.109 kb on + strand at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.122 kb on - strand, within PGA1_c05630 at 576.122 kb on - strand, within PGA1_c05630 at 576.138 kb on - strand, within PGA1_c05630 at 576.148 kb on - strand, within PGA1_c05630 at 576.174 kb on + strand, within PGA1_c05630 at 576.226 kb on - strand, within PGA1_c05630 at 576.226 kb on - strand, within PGA1_c05630 at 576.239 kb on + strand, within PGA1_c05630 at 576.350 kb on + strand, within PGA1_c05630 at 576.466 kb on + strand, within PGA1_c05630 at 576.474 kb on - strand, within PGA1_c05630 at 576.476 kb on + strand, within PGA1_c05630 at 576.484 kb on - strand, within PGA1_c05630 at 576.484 kb on - strand, within PGA1_c05630 at 576.506 kb on - strand at 576.506 kb on - strand at 576.512 kb on + strand at 576.538 kb on + strand at 576.676 kb on + strand, within PGA1_c05640 at 576.714 kb on + strand, within PGA1_c05640 at 576.748 kb on + strand, within PGA1_c05640 at 576.756 kb on - strand, within PGA1_c05640 at 576.756 kb on - strand, within PGA1_c05640 at 576.865 kb on + strand, within PGA1_c05640 at 576.865 kb on + strand, within PGA1_c05640 at 576.873 kb on - strand, within PGA1_c05640 at 576.873 kb on - strand, within PGA1_c05640 at 576.873 kb on - strand, within PGA1_c05640 at 576.873 kb on - strand, within PGA1_c05640 at 577.048 kb on - strand, within PGA1_c05640 at 577.121 kb on - strand, within PGA1_c05640 at 577.121 kb on - strand, within PGA1_c05640 at 577.349 kb on + strand at 577.357 kb on - strand at 577.357 kb on - strand at 577.357 kb on - strand at 577.359 kb on + strand at 577.367 kb on - strand at 577.374 kb on + strand at 577.467 kb on - strand at 577.544 kb on + strand, within PGA1_c05650 at 577.571 kb on - strand, within PGA1_c05650 at 577.637 kb on - strand, within PGA1_c05650 at 578.124 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 575,617 + PGA1_c05620 0.46 -2.6 575,617 + PGA1_c05620 0.46 -1.9 575,617 + PGA1_c05620 0.46 -1.7 575,651 + PGA1_c05620 0.51 -0.5 575,671 + PGA1_c05620 0.53 +2.4 575,684 - PGA1_c05620 0.55 -2.2 575,702 - PGA1_c05620 0.57 -1.9 575,761 + PGA1_c05620 0.65 -0.2 575,761 + PGA1_c05620 0.65 -0.1 575,779 - PGA1_c05620 0.67 -2.7 575,822 + PGA1_c05620 0.72 -0.2 575,884 + PGA1_c05620 0.80 +0.1 575,890 + PGA1_c05620 0.81 +1.0 575,979 + +0.3 575,987 - -1.6 576,016 + +0.6 576,070 + -0.2 576,101 + -0.4 576,109 + -0.2 576,117 - PGA1_c05630 0.10 -0.5 576,117 - PGA1_c05630 0.10 -1.2 576,117 - PGA1_c05630 0.10 +1.7 576,117 - PGA1_c05630 0.10 -1.5 576,117 - PGA1_c05630 0.10 -1.1 576,117 - PGA1_c05630 0.10 -3.0 576,122 - PGA1_c05630 0.11 -0.2 576,122 - PGA1_c05630 0.11 +0.4 576,138 - PGA1_c05630 0.15 +0.7 576,148 - PGA1_c05630 0.17 +0.5 576,174 + PGA1_c05630 0.23 +0.1 576,226 - PGA1_c05630 0.34 +1.1 576,226 - PGA1_c05630 0.34 -0.6 576,239 + PGA1_c05630 0.37 -1.4 576,350 + PGA1_c05630 0.60 -0.3 576,466 + PGA1_c05630 0.85 -0.9 576,474 - PGA1_c05630 0.87 -0.8 576,476 + PGA1_c05630 0.88 +0.7 576,484 - PGA1_c05630 0.89 -0.0 576,484 - PGA1_c05630 0.89 +1.3 576,506 - +2.4 576,506 - +0.8 576,512 + -2.3 576,538 + +0.3 576,676 + PGA1_c05640 0.16 -1.1 576,714 + PGA1_c05640 0.21 -1.4 576,748 + PGA1_c05640 0.26 +1.3 576,756 - PGA1_c05640 0.28 +0.9 576,756 - PGA1_c05640 0.28 -0.7 576,865 + PGA1_c05640 0.44 -1.5 576,865 + PGA1_c05640 0.44 -1.0 576,873 - PGA1_c05640 0.45 -0.8 576,873 - PGA1_c05640 0.45 -1.1 576,873 - PGA1_c05640 0.45 +0.3 576,873 - PGA1_c05640 0.45 -0.7 577,048 - PGA1_c05640 0.71 -1.2 577,121 - PGA1_c05640 0.82 -1.6 577,121 - PGA1_c05640 0.82 +0.3 577,349 + -0.6 577,357 - -0.0 577,357 - +1.3 577,357 - -0.9 577,359 + -0.5 577,367 - -1.2 577,374 + -2.7 577,467 - -0.0 577,544 + PGA1_c05650 0.19 -0.5 577,571 - PGA1_c05650 0.22 -0.2 577,637 - PGA1_c05650 0.31 -1.5 578,124 + -0.5
Or see this region's nucleotide sequence