Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11810 and PGA1_c11820 are separated by 30 nucleotides PGA1_c11820 and PGA1_c11830 are separated by 144 nucleotides PGA1_c11830 and PGA1_c11840 are separated by 73 nucleotides PGA1_c11840 and PGA1_c11850 are separated by 92 nucleotides
PGA1_c11810: PGA1_c11810 - hypothetical protein, at 1,226,146 to 1,226,328
_c11810
PGA1_c11820: PGA1_c11820 - putative esterase, at 1,226,359 to 1,227,117
_c11820
PGA1_c11830: PGA1_c11830 - hypothetical protein, at 1,227,262 to 1,227,627
_c11830
PGA1_c11840: PGA1_c11840 - hypothetical protein, at 1,227,701 to 1,228,288
_c11840
PGA1_c11850: PGA1_c11850 - Phage shock protein A (IM30), suppresses sigma54-dependent transcription, at 1,228,381 to 1,229,058
_c11850
Position (kb)
1227
1228 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1226.292 kb on + strand, within PGA1_c11810 at 1226.355 kb on + strand at 1226.411 kb on + strand at 1226.466 kb on - strand, within PGA1_c11820 at 1226.468 kb on + strand, within PGA1_c11820 at 1226.476 kb on - strand, within PGA1_c11820 at 1226.476 kb on - strand, within PGA1_c11820 at 1226.476 kb on - strand, within PGA1_c11820 at 1226.531 kb on - strand, within PGA1_c11820 at 1226.597 kb on - strand, within PGA1_c11820 at 1226.598 kb on + strand, within PGA1_c11820 at 1226.599 kb on + strand, within PGA1_c11820 at 1226.605 kb on - strand, within PGA1_c11820 at 1226.631 kb on + strand, within PGA1_c11820 at 1226.631 kb on + strand, within PGA1_c11820 at 1226.631 kb on + strand, within PGA1_c11820 at 1226.631 kb on + strand, within PGA1_c11820 at 1226.631 kb on + strand, within PGA1_c11820 at 1226.631 kb on + strand, within PGA1_c11820 at 1226.631 kb on + strand, within PGA1_c11820 at 1226.631 kb on + strand, within PGA1_c11820 at 1226.631 kb on + strand, within PGA1_c11820 at 1226.631 kb on + strand, within PGA1_c11820 at 1226.631 kb on + strand, within PGA1_c11820 at 1226.631 kb on + strand, within PGA1_c11820 at 1226.631 kb on + strand, within PGA1_c11820 at 1226.631 kb on + strand, within PGA1_c11820 at 1226.631 kb on + strand, within PGA1_c11820 at 1226.639 kb on - strand, within PGA1_c11820 at 1226.639 kb on - strand, within PGA1_c11820 at 1226.639 kb on - strand, within PGA1_c11820 at 1226.639 kb on - strand, within PGA1_c11820 at 1226.639 kb on - strand, within PGA1_c11820 at 1226.639 kb on - strand, within PGA1_c11820 at 1226.639 kb on - strand, within PGA1_c11820 at 1226.639 kb on - strand, within PGA1_c11820 at 1226.749 kb on + strand, within PGA1_c11820 at 1226.752 kb on - strand, within PGA1_c11820 at 1226.890 kb on + strand, within PGA1_c11820 at 1226.900 kb on + strand, within PGA1_c11820 at 1226.951 kb on + strand, within PGA1_c11820 at 1226.970 kb on + strand, within PGA1_c11820 at 1227.005 kb on + strand, within PGA1_c11820 at 1227.052 kb on - strand at 1227.054 kb on + strand at 1227.079 kb on - strand at 1227.153 kb on - strand at 1227.207 kb on - strand at 1227.208 kb on - strand at 1227.230 kb on - strand at 1227.256 kb on - strand at 1227.303 kb on + strand, within PGA1_c11830 at 1227.403 kb on - strand, within PGA1_c11830 at 1227.451 kb on + strand, within PGA1_c11830 at 1227.457 kb on - strand, within PGA1_c11830 at 1227.463 kb on + strand, within PGA1_c11830 at 1227.479 kb on + strand, within PGA1_c11830 at 1227.506 kb on + strand, within PGA1_c11830 at 1227.506 kb on - strand, within PGA1_c11830 at 1227.614 kb on - strand at 1227.645 kb on - strand at 1228.268 kb on + strand at 1228.429 kb on + strand at 1228.453 kb on - strand, within PGA1_c11850 at 1228.477 kb on - strand, within PGA1_c11850 at 1228.498 kb on - strand, within PGA1_c11850 at 1228.503 kb on - strand, within PGA1_c11850 at 1228.503 kb on - strand, within PGA1_c11850 at 1228.553 kb on + strand, within PGA1_c11850 at 1228.610 kb on - strand, within PGA1_c11850
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 1,226,292 + PGA1_c11810 0.80 +0.2 1,226,355 + -0.0 1,226,411 + -3.9 1,226,466 - PGA1_c11820 0.14 -0.6 1,226,468 + PGA1_c11820 0.14 +0.2 1,226,476 - PGA1_c11820 0.15 -0.1 1,226,476 - PGA1_c11820 0.15 +0.6 1,226,476 - PGA1_c11820 0.15 -1.8 1,226,531 - PGA1_c11820 0.23 +0.5 1,226,597 - PGA1_c11820 0.31 -0.2 1,226,598 + PGA1_c11820 0.31 +0.5 1,226,599 + PGA1_c11820 0.32 +1.0 1,226,605 - PGA1_c11820 0.32 -1.6 1,226,631 + PGA1_c11820 0.36 +0.7 1,226,631 + PGA1_c11820 0.36 -1.5 1,226,631 + PGA1_c11820 0.36 -0.5 1,226,631 + PGA1_c11820 0.36 -1.4 1,226,631 + PGA1_c11820 0.36 +1.5 1,226,631 + PGA1_c11820 0.36 -0.1 1,226,631 + PGA1_c11820 0.36 -0.3 1,226,631 + PGA1_c11820 0.36 -1.3 1,226,631 + PGA1_c11820 0.36 -1.1 1,226,631 + PGA1_c11820 0.36 +1.2 1,226,631 + PGA1_c11820 0.36 -0.9 1,226,631 + PGA1_c11820 0.36 -0.2 1,226,631 + PGA1_c11820 0.36 +0.9 1,226,631 + PGA1_c11820 0.36 +0.9 1,226,631 + PGA1_c11820 0.36 +0.2 1,226,639 - PGA1_c11820 0.37 -0.2 1,226,639 - PGA1_c11820 0.37 -0.7 1,226,639 - PGA1_c11820 0.37 -1.3 1,226,639 - PGA1_c11820 0.37 -0.4 1,226,639 - PGA1_c11820 0.37 +0.4 1,226,639 - PGA1_c11820 0.37 +0.2 1,226,639 - PGA1_c11820 0.37 -0.0 1,226,639 - PGA1_c11820 0.37 +0.1 1,226,749 + PGA1_c11820 0.51 +0.7 1,226,752 - PGA1_c11820 0.52 +2.8 1,226,890 + PGA1_c11820 0.70 -2.0 1,226,900 + PGA1_c11820 0.71 -1.1 1,226,951 + PGA1_c11820 0.78 -1.5 1,226,970 + PGA1_c11820 0.81 +0.6 1,227,005 + PGA1_c11820 0.85 +1.2 1,227,052 - +0.8 1,227,054 + +0.4 1,227,079 - -1.3 1,227,153 - +1.2 1,227,207 - -0.7 1,227,208 - -1.5 1,227,230 - +0.5 1,227,256 - +1.1 1,227,303 + PGA1_c11830 0.11 +1.2 1,227,403 - PGA1_c11830 0.39 -0.4 1,227,451 + PGA1_c11830 0.52 -0.8 1,227,457 - PGA1_c11830 0.53 -1.6 1,227,463 + PGA1_c11830 0.55 -1.6 1,227,479 + PGA1_c11830 0.59 -2.0 1,227,506 + PGA1_c11830 0.67 -0.1 1,227,506 - PGA1_c11830 0.67 +0.5 1,227,614 - +0.5 1,227,645 - +0.3 1,228,268 + -1.1 1,228,429 + +0.1 1,228,453 - PGA1_c11850 0.11 -1.9 1,228,477 - PGA1_c11850 0.14 -0.1 1,228,498 - PGA1_c11850 0.17 +1.6 1,228,503 - PGA1_c11850 0.18 +0.1 1,228,503 - PGA1_c11850 0.18 -0.5 1,228,553 + PGA1_c11850 0.25 -2.9 1,228,610 - PGA1_c11850 0.34 +0.5
Or see this region's nucleotide sequence