Experiment: propionate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3146 and Dshi_3147 are separated by 34 nucleotides Dshi_3147 and Dshi_3148 are separated by 2 nucleotides Dshi_3148 and Dshi_3149 overlap by 4 nucleotides Dshi_3149 and Dshi_3150 are separated by 104 nucleotides
Dshi_3146: Dshi_3146 - acetyl-CoA carboxylase, carboxyl transferase, alpha subunit (RefSeq), at 3,312,960 to 3,313,922
_3146
Dshi_3147: Dshi_3147 - protein of unknown function DUF306 Meta and HslJ (RefSeq), at 3,313,957 to 3,314,697
_3147
Dshi_3148: Dshi_3148 - hypothetical protein (RefSeq), at 3,314,700 to 3,315,176
_3148
Dshi_3149: Dshi_3149 - peptidase M15D vanX D-ala-D-ala dipeptidase (RefSeq), at 3,315,173 to 3,315,985
_3149
Dshi_3150: Dshi_3150 - aminotransferase class IV (RefSeq), at 3,316,090 to 3,316,944
_3150
Position (kb)
3314
3315
3316 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3314.091 kb on + strand, within Dshi_3147 at 3314.091 kb on - strand, within Dshi_3147 at 3314.228 kb on + strand, within Dshi_3147 at 3314.250 kb on + strand, within Dshi_3147 at 3314.341 kb on - strand, within Dshi_3147 at 3314.418 kb on - strand, within Dshi_3147 at 3314.982 kb on + strand, within Dshi_3148 at 3315.075 kb on - strand, within Dshi_3148 at 3315.081 kb on + strand, within Dshi_3148 at 3315.186 kb on + strand at 3315.191 kb on + strand at 3315.227 kb on + strand at 3315.294 kb on + strand, within Dshi_3149 at 3315.375 kb on + strand, within Dshi_3149 at 3315.385 kb on + strand, within Dshi_3149 at 3315.424 kb on - strand, within Dshi_3149 at 3315.487 kb on - strand, within Dshi_3149 at 3315.687 kb on + strand, within Dshi_3149 at 3315.807 kb on + strand, within Dshi_3149 at 3315.849 kb on + strand, within Dshi_3149 at 3315.978 kb on + strand at 3316.052 kb on + strand at 3316.085 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction propionate (C) remove 3,314,091 + Dshi_3147 0.18 -1.2 3,314,091 - Dshi_3147 0.18 -2.1 3,314,228 + Dshi_3147 0.37 -0.4 3,314,250 + Dshi_3147 0.40 -0.5 3,314,341 - Dshi_3147 0.52 -0.5 3,314,418 - Dshi_3147 0.62 +0.2 3,314,982 + Dshi_3148 0.59 -0.6 3,315,075 - Dshi_3148 0.79 -0.7 3,315,081 + Dshi_3148 0.80 -0.6 3,315,186 + -0.0 3,315,191 + -0.8 3,315,227 + -0.2 3,315,294 + Dshi_3149 0.15 -0.4 3,315,375 + Dshi_3149 0.25 +0.1 3,315,385 + Dshi_3149 0.26 -2.2 3,315,424 - Dshi_3149 0.31 -1.7 3,315,487 - Dshi_3149 0.39 -0.3 3,315,687 + Dshi_3149 0.63 -0.3 3,315,807 + Dshi_3149 0.78 -1.1 3,315,849 + Dshi_3149 0.83 -1.4 3,315,978 + +0.5 3,316,052 + +0.4 3,316,085 + -3.7
Or see this region's nucleotide sequence