Experiment: succinate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2627 and Dshi_2628 overlap by 4 nucleotides Dshi_2628 and Dshi_2629 are separated by 27 nucleotides Dshi_2629 and Dshi_2630 are separated by 3 nucleotides Dshi_2630 and Dshi_2631 are separated by 131 nucleotides
Dshi_2627: Dshi_2627 - nitroreductase (RefSeq), at 2,785,328 to 2,785,978
_2627
Dshi_2628: Dshi_2628 - hypothetical protein (RefSeq), at 2,785,975 to 2,786,685
_2628
Dshi_2629: Dshi_2629 - protein of unknown function DUF1467 (RefSeq), at 2,786,713 to 2,787,000
_2629
Dshi_2630: Dshi_2630 - Methylmalonyl-CoA epimerase (RefSeq), at 2,787,004 to 2,787,408
_2630
Dshi_2631: Dshi_2631 - response regulator receiver protein (RefSeq), at 2,787,540 to 2,788,244
_2631
Position (kb)
2786
2787
2788 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2785.821 kb on + strand, within Dshi_2627 at 2785.931 kb on - strand at 2786.043 kb on + strand at 2786.375 kb on - strand, within Dshi_2628 at 2786.455 kb on + strand, within Dshi_2628 at 2786.665 kb on - strand at 2786.743 kb on - strand, within Dshi_2629 at 2786.984 kb on - strand at 2787.002 kb on + strand at 2787.002 kb on + strand at 2787.010 kb on - strand at 2787.425 kb on - strand at 2787.451 kb on + strand at 2787.544 kb on - strand at 2787.765 kb on - strand, within Dshi_2631 at 2787.863 kb on + strand, within Dshi_2631 at 2787.920 kb on + strand, within Dshi_2631
Per-strain Table
Position Strand Gene LocusTag Fraction succinate (C) remove 2,785,821 + Dshi_2627 0.76 -2.4 2,785,931 - -1.2 2,786,043 + +0.7 2,786,375 - Dshi_2628 0.56 -0.1 2,786,455 + Dshi_2628 0.68 +2.3 2,786,665 - -0.5 2,786,743 - Dshi_2629 0.10 -1.5 2,786,984 - -2.0 2,787,002 + -0.7 2,787,002 + +0.2 2,787,010 - -0.5 2,787,425 - +0.5 2,787,451 + -0.1 2,787,544 - +0.2 2,787,765 - Dshi_2631 0.32 +0.4 2,787,863 + Dshi_2631 0.46 -0.2 2,787,920 + Dshi_2631 0.54 +0.3
Or see this region's nucleotide sequence