Experiment: L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisC and hisD are separated by 62 nucleotides hisD and hisG are separated by 3 nucleotides
SO2072: hisC - histidinol-phosphate aminotransferase (NCBI ptt file), at 2,168,394 to 2,169,569
hisC
SO2073: hisD - histidinol dehydrogenase (NCBI ptt file), at 2,169,632 to 2,170,948
hisD
SO2074: hisG - ATP phosphoribosyltransferase (NCBI ptt file), at 2,170,952 to 2,171,926
hisG
Position (kb)
2169
2170
2171 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2168.737 kb on + strand, within hisC at 2168.737 kb on + strand, within hisC at 2168.777 kb on + strand, within hisC at 2168.883 kb on + strand, within hisC at 2168.891 kb on - strand, within hisC at 2168.913 kb on - strand, within hisC at 2168.951 kb on - strand, within hisC at 2168.951 kb on - strand, within hisC at 2168.951 kb on - strand, within hisC at 2169.078 kb on + strand, within hisC at 2169.098 kb on + strand, within hisC at 2169.098 kb on + strand, within hisC at 2169.098 kb on + strand, within hisC at 2169.098 kb on + strand, within hisC at 2169.098 kb on + strand, within hisC at 2169.098 kb on + strand, within hisC at 2169.098 kb on + strand, within hisC at 2169.106 kb on - strand, within hisC at 2169.106 kb on - strand, within hisC at 2169.148 kb on - strand, within hisC at 2169.263 kb on + strand, within hisC at 2169.542 kb on - strand at 2169.597 kb on + strand at 2169.619 kb on - strand at 2169.757 kb on + strand at 2169.765 kb on - strand, within hisD at 2169.791 kb on + strand, within hisD at 2169.799 kb on - strand, within hisD at 2169.960 kb on - strand, within hisD at 2170.143 kb on - strand, within hisD at 2170.148 kb on - strand, within hisD at 2170.173 kb on + strand at 2170.208 kb on + strand, within hisD at 2170.216 kb on - strand, within hisD at 2170.254 kb on - strand, within hisD at 2170.370 kb on - strand, within hisD at 2170.476 kb on - strand, within hisD at 2170.496 kb on + strand, within hisD at 2170.510 kb on - strand, within hisD at 2170.771 kb on + strand, within hisD at 2170.885 kb on - strand at 2170.984 kb on + strand at 2171.004 kb on + strand at 2171.014 kb on + strand at 2171.014 kb on + strand at 2171.046 kb on - strand at 2171.207 kb on + strand, within hisG at 2171.257 kb on - strand, within hisG at 2171.293 kb on + strand, within hisG at 2171.360 kb on + strand, within hisG at 2171.526 kb on + strand, within hisG at 2171.534 kb on - strand, within hisG at 2171.595 kb on - strand, within hisG at 2171.785 kb on + strand, within hisG at 2171.862 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate (C) remove 2,168,737 + hisC SO2072 0.29 -2.8 2,168,737 + hisC SO2072 0.29 -3.4 2,168,777 + hisC SO2072 0.33 -3.9 2,168,883 + hisC SO2072 0.42 -3.4 2,168,891 - hisC SO2072 0.42 -4.3 2,168,913 - hisC SO2072 0.44 -4.3 2,168,951 - hisC SO2072 0.47 -3.4 2,168,951 - hisC SO2072 0.47 -3.8 2,168,951 - hisC SO2072 0.47 -4.0 2,169,078 + hisC SO2072 0.58 -2.4 2,169,098 + hisC SO2072 0.60 -1.6 2,169,098 + hisC SO2072 0.60 -1.0 2,169,098 + hisC SO2072 0.60 -2.0 2,169,098 + hisC SO2072 0.60 -2.0 2,169,098 + hisC SO2072 0.60 -2.0 2,169,098 + hisC SO2072 0.60 -3.4 2,169,098 + hisC SO2072 0.60 -3.4 2,169,106 - hisC SO2072 0.61 -2.4 2,169,106 - hisC SO2072 0.61 -3.0 2,169,148 - hisC SO2072 0.64 -1.6 2,169,263 + hisC SO2072 0.74 -4.7 2,169,542 - -3.5 2,169,597 + -4.3 2,169,619 - -1.4 2,169,757 + -5.0 2,169,765 - hisD SO2073 0.10 -1.0 2,169,791 + hisD SO2073 0.12 -5.4 2,169,799 - hisD SO2073 0.13 -5.3 2,169,960 - hisD SO2073 0.25 -4.1 2,170,143 - hisD SO2073 0.39 -2.0 2,170,148 - hisD SO2073 0.39 -5.0 2,170,173 + -1.6 2,170,208 + hisD SO2073 0.44 -4.9 2,170,216 - hisD SO2073 0.44 -3.9 2,170,254 - hisD SO2073 0.47 -4.7 2,170,370 - hisD SO2073 0.56 -4.0 2,170,476 - hisD SO2073 0.64 -4.3 2,170,496 + hisD SO2073 0.66 -4.0 2,170,510 - hisD SO2073 0.67 -4.3 2,170,771 + hisD SO2073 0.86 -4.7 2,170,885 - -3.7 2,170,984 + -3.5 2,171,004 + -3.5 2,171,014 + -4.1 2,171,014 + -3.4 2,171,046 - -0.9 2,171,207 + hisG SO2074 0.26 -4.0 2,171,257 - hisG SO2074 0.31 -5.0 2,171,293 + hisG SO2074 0.35 -4.5 2,171,360 + hisG SO2074 0.42 -3.4 2,171,526 + hisG SO2074 0.59 -5.0 2,171,534 - hisG SO2074 0.60 -4.0 2,171,595 - hisG SO2074 0.66 -3.6 2,171,785 + hisG SO2074 0.85 -2.6 2,171,862 + -3.6
Or see this region's nucleotide sequence