Experiment: L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pfs and SO1323 overlap by 2 nucleotides SO1323 and gltD are separated by 62 nucleotides gltD and gltB are separated by 6 nucleotides
SO1322: pfs - 5-methylthioadenosine nucleosidase/S-adenosylhomocysteine nucleosidase (NCBI ptt file), at 1,375,956 to 1,376,666
pfs
SO1323: SO1323 - hypothetical protein (NCBI ptt file), at 1,376,665 to 1,376,871
SO1323
SO1324: gltD - glutamate synthase, small subunit (NCBI ptt file), at 1,376,934 to 1,378,340
gltD
SO1325: gltB - glutamate synthase, large subunit (NCBI ptt file), at 1,378,347 to 1,382,795
gltB
Position (kb)
1376
1377
1378
1379 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1376.761 kb on + strand, within SO1323 at 1376.816 kb on - strand, within SO1323 at 1376.823 kb on + strand, within SO1323 at 1376.823 kb on + strand, within SO1323 at 1376.830 kb on - strand, within SO1323 at 1376.873 kb on - strand at 1376.895 kb on + strand at 1376.999 kb on + strand at 1377.008 kb on - strand at 1377.165 kb on - strand, within gltD at 1377.203 kb on - strand, within gltD at 1377.343 kb on - strand, within gltD at 1377.381 kb on - strand, within gltD at 1377.383 kb on + strand, within gltD at 1377.419 kb on - strand, within gltD at 1377.502 kb on - strand, within gltD at 1377.502 kb on - strand, within gltD at 1377.502 kb on - strand, within gltD at 1377.558 kb on + strand, within gltD at 1377.723 kb on + strand, within gltD at 1377.723 kb on + strand, within gltD at 1377.731 kb on - strand, within gltD at 1377.731 kb on - strand, within gltD at 1377.762 kb on - strand, within gltD at 1377.772 kb on - strand, within gltD at 1377.787 kb on - strand, within gltD at 1377.864 kb on + strand, within gltD at 1377.864 kb on + strand, within gltD at 1377.864 kb on + strand, within gltD at 1377.864 kb on + strand, within gltD at 1377.872 kb on - strand, within gltD at 1377.872 kb on - strand, within gltD at 1377.872 kb on - strand, within gltD at 1377.872 kb on - strand, within gltD at 1377.872 kb on - strand, within gltD at 1377.914 kb on + strand, within gltD at 1377.932 kb on - strand, within gltD at 1377.942 kb on + strand, within gltD at 1378.035 kb on - strand, within gltD at 1378.048 kb on + strand, within gltD at 1378.076 kb on + strand, within gltD at 1378.084 kb on - strand, within gltD at 1378.100 kb on - strand, within gltD at 1378.178 kb on - strand, within gltD at 1378.180 kb on + strand, within gltD at 1378.180 kb on + strand, within gltD at 1378.180 kb on + strand, within gltD at 1378.180 kb on + strand, within gltD at 1378.180 kb on + strand, within gltD at 1378.180 kb on + strand, within gltD at 1378.188 kb on - strand, within gltD at 1378.188 kb on - strand, within gltD at 1378.188 kb on - strand, within gltD at 1378.188 kb on - strand, within gltD at 1378.237 kb on + strand at 1378.382 kb on + strand at 1378.390 kb on - strand at 1378.390 kb on - strand at 1378.439 kb on + strand at 1378.719 kb on + strand at 1378.727 kb on - strand at 1378.744 kb on + strand at 1378.770 kb on + strand at 1378.770 kb on + strand at 1378.863 kb on - strand, within gltB at 1378.940 kb on + strand, within gltB at 1378.940 kb on - strand, within gltB at 1378.959 kb on - strand, within gltB at 1378.971 kb on + strand, within gltB at 1378.979 kb on - strand, within gltB at 1379.021 kb on + strand, within gltB at 1379.057 kb on + strand, within gltB at 1379.080 kb on + strand, within gltB
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate (C) remove 1,376,761 + SO1323 0.46 +0.3 1,376,816 - SO1323 0.73 +0.5 1,376,823 + SO1323 0.76 +0.3 1,376,823 + SO1323 0.76 -0.9 1,376,830 - SO1323 0.80 -0.6 1,376,873 - -1.0 1,376,895 + +0.8 1,376,999 + -3.8 1,377,008 - -3.5 1,377,165 - gltD SO1324 0.16 -4.8 1,377,203 - gltD SO1324 0.19 -5.9 1,377,343 - gltD SO1324 0.29 -1.7 1,377,381 - gltD SO1324 0.32 -2.6 1,377,383 + gltD SO1324 0.32 -2.1 1,377,419 - gltD SO1324 0.34 -3.7 1,377,502 - gltD SO1324 0.40 -3.8 1,377,502 - gltD SO1324 0.40 -2.1 1,377,502 - gltD SO1324 0.40 -3.6 1,377,558 + gltD SO1324 0.44 -4.4 1,377,723 + gltD SO1324 0.56 -3.1 1,377,723 + gltD SO1324 0.56 -3.8 1,377,731 - gltD SO1324 0.57 -3.4 1,377,731 - gltD SO1324 0.57 -2.6 1,377,762 - gltD SO1324 0.59 -4.9 1,377,772 - gltD SO1324 0.60 -3.7 1,377,787 - gltD SO1324 0.61 -3.4 1,377,864 + gltD SO1324 0.66 -3.6 1,377,864 + gltD SO1324 0.66 -3.3 1,377,864 + gltD SO1324 0.66 -4.1 1,377,864 + gltD SO1324 0.66 -3.7 1,377,872 - gltD SO1324 0.67 -3.1 1,377,872 - gltD SO1324 0.67 -2.6 1,377,872 - gltD SO1324 0.67 -3.6 1,377,872 - gltD SO1324 0.67 -3.9 1,377,872 - gltD SO1324 0.67 -6.1 1,377,914 + gltD SO1324 0.70 -3.4 1,377,932 - gltD SO1324 0.71 -3.3 1,377,942 + gltD SO1324 0.72 -4.9 1,378,035 - gltD SO1324 0.78 -2.7 1,378,048 + gltD SO1324 0.79 -1.7 1,378,076 + gltD SO1324 0.81 -4.0 1,378,084 - gltD SO1324 0.82 -2.3 1,378,100 - gltD SO1324 0.83 -4.1 1,378,178 - gltD SO1324 0.88 -4.7 1,378,180 + gltD SO1324 0.89 -4.5 1,378,180 + gltD SO1324 0.89 -4.8 1,378,180 + gltD SO1324 0.89 -4.2 1,378,180 + gltD SO1324 0.89 -5.0 1,378,180 + gltD SO1324 0.89 -1.3 1,378,180 + gltD SO1324 0.89 -4.4 1,378,188 - gltD SO1324 0.89 -4.0 1,378,188 - gltD SO1324 0.89 -5.3 1,378,188 - gltD SO1324 0.89 -0.7 1,378,188 - gltD SO1324 0.89 -5.0 1,378,237 + -3.7 1,378,382 + -3.7 1,378,390 - -0.7 1,378,390 - -2.7 1,378,439 + -3.4 1,378,719 + -4.3 1,378,727 - -4.9 1,378,744 + -3.4 1,378,770 + -4.6 1,378,770 + -3.9 1,378,863 - gltB SO1325 0.12 -5.3 1,378,940 + gltB SO1325 0.13 -3.8 1,378,940 - gltB SO1325 0.13 -4.3 1,378,959 - gltB SO1325 0.14 -4.8 1,378,971 + gltB SO1325 0.14 -2.3 1,378,979 - gltB SO1325 0.14 -4.2 1,379,021 + gltB SO1325 0.15 -4.8 1,379,057 + gltB SO1325 0.16 -2.3 1,379,080 + gltB SO1325 0.16 -4.7
Or see this region's nucleotide sequence