Strain Fitness in Dinoroseobacter shibae DFL-12 around Dshi_3220

Experiment: pyruvate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntDshi_3218 and Dshi_3219 overlap by 4 nucleotidesDshi_3219 and Dshi_3220 are separated by 4 nucleotidesDshi_3220 and Dshi_3221 are separated by 31 nucleotidesDshi_3221 and 11487655 are separated by 63 nucleotides11487655 and 11630018 overlap by 29 nucleotides11630018 and 11772410 overlap by 29 nucleotides11772410 and 11487656 are separated by 0 nucleotides11487656 and 11630019 overlap by 32 nucleotides11630019 and 11772411 overlap by 32 nucleotides11772411 and 11487657 are separated by 0 nucleotides11487657 and 11630020 overlap by 29 nucleotides11630020 and 11772412 overlap by 29 nucleotides11772412 and 11487658 are separated by 0 nucleotides11487658 and 11630021 overlap by 32 nucleotides11630021 and 11772413 overlap by 32 nucleotides11772413 and 11487659 are separated by 0 nucleotides11487659 and 11630022 overlap by 29 nucleotides11630022 and 11772414 overlap by 29 nucleotides11772414 and 11487660 are separated by 0 nucleotides11487660 and 11630023 overlap by 32 nucleotides11630023 and 11772415 overlap by 32 nucleotides11772415 and 11487661 are separated by 0 nucleotides11487661 and 11630024 overlap by 29 nucleotides11630024 and 11772416 overlap by 29 nucleotides11772416 and 11487662 are separated by 0 nucleotides11487662 and 11630025 overlap by 32 nucleotides11630025 and 11772417 overlap by 32 nucleotides11772417 and 11487663 are separated by 0 nucleotides11487663 and 11630026 overlap by 29 nucleotides11630026 and 11772418 overlap by 29 nucleotides11772418 and 11487664 are separated by 0 nucleotides11487664 and 11630027 overlap by 32 nucleotides11630027 and 11772419 overlap by 32 nucleotides11772419 and 11487665 are separated by 0 nucleotides11487665 and 11630028 overlap by 29 nucleotides11630028 and 11772420 overlap by 29 nucleotides11772420 and 11487666 are separated by 0 nucleotides11487666 and 11630029 overlap by 32 nucleotides11630029 and 11772421 overlap by 32 nucleotides11772421 and 11487667 are separated by 0 nucleotides11487667 and 11630030 overlap by 29 nucleotides11630030 and 11772422 overlap by 29 nucleotides11772422 and 11487668 are separated by 0 nucleotides11487668 and 11630031 overlap by 32 nucleotides11630031 and 11772423 overlap by 32 nucleotides11772423 and 11487669 are separated by 0 nucleotides11487669 and 11630032 overlap by 29 nucleotides11630032 and 11772424 overlap by 29 nucleotides11772424 and 11487670 are separated by 0 nucleotides11487670 and 11630033 overlap by 32 nucleotides11630033 and 11772425 overlap by 32 nucleotides11772425 and 11487671 are separated by 0 nucleotides11487671 and 11630034 overlap by 29 nucleotides11630034 and 11772426 overlap by 29 nucleotides11772426 and 11487672 are separated by 0 nucleotides11487672 and 11630035 overlap by 32 nucleotides11630035 and 11772427 overlap by 32 nucleotides11772427 and 11487673 are separated by 0 nucleotides11487673 and 11630036 overlap by 29 nucleotides11630036 and 11772428 overlap by 29 nucleotides11772428 and 11487674 are separated by 0 nucleotides11487674 and 11630037 overlap by 32 nucleotides11630037 and 11772429 overlap by 32 nucleotides11772429 and 11487675 are separated by 0 nucleotides Dshi_3218: Dshi_3218 - CRISPR-associated protein Cas5 family (RefSeq), at 3,387,476 to 3,388,162 _3218 Dshi_3219: Dshi_3219 - CRISPR-associated protein CT1974 (RefSeq), at 3,388,159 to 3,388,950 _3219 Dshi_3220: Dshi_3220 - CRISPR-associated protein Cas1 (RefSeq), at 3,388,955 to 3,389,830 _3220 Dshi_3221: Dshi_3221 - CRISPR-associated protein Cas2 (RefSeq), at 3,389,862 to 3,390,155 _3221 11487655 - no description, at 3,390,219 to 3,390,247 11487655 11630018 - no description, at 3,390,219 to 3,390,247 11630018 11772410 - no description, at 3,390,219 to 3,390,247 11772410 11487656 - no description, at 3,390,248 to 3,390,279 11487656 11630019 - no description, at 3,390,248 to 3,390,279 11630019 11772411 - no description, at 3,390,248 to 3,390,279 11772411 11487657 - no description, at 3,390,280 to 3,390,308 11487657 11630020 - no description, at 3,390,280 to 3,390,308 11630020 11772412 - no description, at 3,390,280 to 3,390,308 11772412 11487658 - no description, at 3,390,309 to 3,390,340 11487658 11630021 - no description, at 3,390,309 to 3,390,340 11630021 11772413 - no description, at 3,390,309 to 3,390,340 11772413 11487659 - no description, at 3,390,341 to 3,390,369 11487659 11630022 - no description, at 3,390,341 to 3,390,369 11630022 11772414 - no description, at 3,390,341 to 3,390,369 11772414 11487660 - no description, at 3,390,370 to 3,390,401 11487660 11630023 - no description, at 3,390,370 to 3,390,401 11630023 11772415 - no description, at 3,390,370 to 3,390,401 11772415 11487661 - no description, at 3,390,402 to 3,390,430 11487661 11630024 - no description, at 3,390,402 to 3,390,430 11630024 11772416 - no description, at 3,390,402 to 3,390,430 11772416 11487662 - no description, at 3,390,431 to 3,390,462 11487662 11630025 - no description, at 3,390,431 to 3,390,462 11630025 11772417 - no description, at 3,390,431 to 3,390,462 11772417 11487663 - no description, at 3,390,463 to 3,390,491 11487663 11630026 - no description, at 3,390,463 to 3,390,491 11630026 11772418 - no description, at 3,390,463 to 3,390,491 11772418 11487664 - no description, at 3,390,492 to 3,390,523 11487664 11630027 - no description, at 3,390,492 to 3,390,523 11630027 11772419 - no description, at 3,390,492 to 3,390,523 11772419 11487665 - no description, at 3,390,524 to 3,390,552 11487665 11630028 - no description, at 3,390,524 to 3,390,552 11630028 11772420 - no description, at 3,390,524 to 3,390,552 11772420 11487666 - no description, at 3,390,553 to 3,390,584 11487666 11630029 - no description, at 3,390,553 to 3,390,584 11630029 11772421 - no description, at 3,390,553 to 3,390,584 11772421 11487667 - no description, at 3,390,585 to 3,390,613 11487667 11630030 - no description, at 3,390,585 to 3,390,613 11630030 11772422 - no description, at 3,390,585 to 3,390,613 11772422 11487668 - no description, at 3,390,614 to 3,390,645 11487668 11630031 - no description, at 3,390,614 to 3,390,645 11630031 11772423 - no description, at 3,390,614 to 3,390,645 11772423 11487669 - no description, at 3,390,646 to 3,390,674 11487669 11630032 - no description, at 3,390,646 to 3,390,674 11630032 11772424 - no description, at 3,390,646 to 3,390,674 11772424 11487670 - no description, at 3,390,675 to 3,390,706 11487670 11630033 - no description, at 3,390,675 to 3,390,706 11630033 11772425 - no description, at 3,390,675 to 3,390,706 11772425 11487671 - no description, at 3,390,707 to 3,390,735 11487671 11630034 - no description, at 3,390,707 to 3,390,735 11630034 11772426 - no description, at 3,390,707 to 3,390,735 11772426 11487672 - no description, at 3,390,736 to 3,390,767 11487672 11630035 - no description, at 3,390,736 to 3,390,767 11630035 11772427 - no description, at 3,390,736 to 3,390,767 11772427 11487673 - no description, at 3,390,768 to 3,390,796 11487673 11630036 - no description, at 3,390,768 to 3,390,796 11630036 11772428 - no description, at 3,390,768 to 3,390,796 11772428 11487674 - no description, at 3,390,797 to 3,390,828 11487674 11630037 - no description, at 3,390,797 to 3,390,828 11630037 11772429 - no description, at 3,390,797 to 3,390,828 11772429 11487675 - no description, at 3,390,829 to 3,390,857 11487675 11630038 - no description, at 3,390,829 to 3,390,857 11630038 11772430 - no description, at 3,390,829 to 3,390,857 11772430 Position (kb) 3388 3389 3390Strain fitness (log2 ratio) -2 -1 0 1 2at 3387.991 kb on - strand, within Dshi_3218at 3388.031 kb on + strand, within Dshi_3218at 3388.078 kb on - strand, within Dshi_3218at 3388.105 kb on + strandat 3388.202 kb on - strandat 3388.221 kb on - strandat 3388.221 kb on - strandat 3388.260 kb on + strand, within Dshi_3219at 3388.286 kb on - strand, within Dshi_3219at 3388.365 kb on - strand, within Dshi_3219at 3388.445 kb on + strand, within Dshi_3219at 3388.453 kb on - strand, within Dshi_3219at 3388.545 kb on + strand, within Dshi_3219at 3388.551 kb on + strand, within Dshi_3219at 3388.635 kb on + strand, within Dshi_3219at 3388.662 kb on - strand, within Dshi_3219at 3388.662 kb on - strand, within Dshi_3219at 3388.909 kb on + strandat 3389.012 kb on + strandat 3389.012 kb on + strandat 3389.097 kb on + strand, within Dshi_3220at 3389.181 kb on - strand, within Dshi_3220at 3389.189 kb on - strand, within Dshi_3220at 3389.242 kb on + strand, within Dshi_3220at 3389.305 kb on - strand, within Dshi_3220at 3389.310 kb on - strand, within Dshi_3220at 3389.322 kb on + strand, within Dshi_3220at 3389.341 kb on + strand, within Dshi_3220at 3389.344 kb on + strand, within Dshi_3220at 3389.380 kb on - strand, within Dshi_3220at 3389.393 kb on - strand, within Dshi_3220at 3389.419 kb on + strand, within Dshi_3220at 3389.502 kb on + strand, within Dshi_3220at 3389.519 kb on - strand, within Dshi_3220at 3389.600 kb on + strand, within Dshi_3220at 3389.674 kb on + strand, within Dshi_3220at 3389.807 kb on + strandat 3389.824 kb on + strandat 3389.829 kb on - strandat 3389.967 kb on - strand, within Dshi_3221at 3389.969 kb on + strand, within Dshi_3221at 3389.990 kb on + strand, within Dshi_3221at 3389.990 kb on + strand, within Dshi_3221at 3389.998 kb on - strand, within Dshi_3221at 3389.998 kb on - strand, within Dshi_3221at 3390.076 kb on + strand, within Dshi_3221at 3390.100 kb on + strand, within Dshi_3221at 3390.233 kb on + strandat 3390.238 kb on + strandat 3390.263 kb on - strandat 3390.279 kb on + strandat 3390.281 kb on - strandat 3390.292 kb on - strandat 3390.322 kb on - strandat 3390.323 kb on - strandat 3390.391 kb on + strandat 3390.397 kb on - strandat 3390.457 kb on + strandat 3390.465 kb on - strandat 3390.491 kb on - strandat 3390.575 kb on - strandat 3390.735 kb on + strandat 3390.743 kb on - strandat 3390.816 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction pyruvate (C)
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3,387,991 - Dshi_3218 0.75 -0.5
3,388,031 + Dshi_3218 0.81 +1.8
3,388,078 - Dshi_3218 0.88 -0.6
3,388,105 + +0.5
3,388,202 - -0.4
3,388,221 - -0.2
3,388,221 - -1.0
3,388,260 + Dshi_3219 0.13 +2.1
3,388,286 - Dshi_3219 0.16 -2.1
3,388,365 - Dshi_3219 0.26 +0.9
3,388,445 + Dshi_3219 0.36 -0.1
3,388,453 - Dshi_3219 0.37 +1.4
3,388,545 + Dshi_3219 0.49 -0.2
3,388,551 + Dshi_3219 0.49 +0.1
3,388,635 + Dshi_3219 0.60 +0.2
3,388,662 - Dshi_3219 0.64 +0.8
3,388,662 - Dshi_3219 0.64 -1.3
3,388,909 + +0.8
3,389,012 + +0.3
3,389,012 + -0.7
3,389,097 + Dshi_3220 0.16 +0.3
3,389,181 - Dshi_3220 0.26 +1.0
3,389,189 - Dshi_3220 0.27 +0.8
3,389,242 + Dshi_3220 0.33 -1.0
3,389,305 - Dshi_3220 0.40 -0.2
3,389,310 - Dshi_3220 0.41 +0.9
3,389,322 + Dshi_3220 0.42 +1.1
3,389,341 + Dshi_3220 0.44 +0.6
3,389,344 + Dshi_3220 0.44 -2.5
3,389,380 - Dshi_3220 0.49 +0.8
3,389,393 - Dshi_3220 0.50 +0.1
3,389,419 + Dshi_3220 0.53 +0.7
3,389,502 + Dshi_3220 0.62 -0.4
3,389,519 - Dshi_3220 0.64 +0.2
3,389,600 + Dshi_3220 0.74 +1.4
3,389,674 + Dshi_3220 0.82 +0.8
3,389,807 + -0.2
3,389,824 + +0.2
3,389,829 - +0.3
3,389,967 - Dshi_3221 0.36 -0.1
3,389,969 + Dshi_3221 0.36 -0.6
3,389,990 + Dshi_3221 0.44 -1.5
3,389,990 + Dshi_3221 0.44 -2.2
3,389,998 - Dshi_3221 0.46 +0.3
3,389,998 - Dshi_3221 0.46 -0.0
3,390,076 + Dshi_3221 0.73 +0.5
3,390,100 + Dshi_3221 0.81 +0.1
3,390,233 + -0.2
3,390,238 + -0.5
3,390,263 - +0.1
3,390,279 + +0.1
3,390,281 - +1.8
3,390,292 - +0.4
3,390,322 - +0.5
3,390,323 - +0.9
3,390,391 + -0.6
3,390,397 - +1.1
3,390,457 + -0.3
3,390,465 - -0.1
3,390,491 - +0.3
3,390,575 - -0.1
3,390,735 + +0.0
3,390,743 - +1.3
3,390,816 + +0.1

Or see this region's nucleotide sequence