Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2249 and Dshi_2250 overlap by 4 nucleotides Dshi_2250 and Dshi_2251 are separated by 2 nucleotides Dshi_2251 and Dshi_2252 are separated by 138 nucleotides Dshi_2252 and Dshi_2253 are separated by 186 nucleotides
Dshi_2249: Dshi_2249 - NusB antitermination factor (RefSeq), at 2,370,535 to 2,371,014
_2249
Dshi_2250: Dshi_2250 - 6,7-dimethyl-8-ribityllumazine synthase (RefSeq), at 2,371,011 to 2,371,562
_2250
Dshi_2251: Dshi_2251 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (RefSeq), at 2,371,565 to 2,372,680
_2251
Dshi_2252: Dshi_2252 - riboflavin synthase, alpha subunit (RefSeq), at 2,372,819 to 2,373,421
_2252
Dshi_2253: Dshi_2253 - Capsule polysaccharide biosynthesis protein (RefSeq), at 2,373,608 to 2,374,894
_2253
Position (kb)
2371
2372
2373 Strain fitness (log2 ratio)
-2
-1
0
1 at 2372.769 kb on - strand at 2373.422 kb on + strand at 2373.429 kb on - strand at 2373.429 kb on - strand at 2373.447 kb on - strand at 2373.474 kb on + strand at 2373.485 kb on + strand at 2373.493 kb on - strand at 2373.545 kb on - strand at 2373.604 kb on + strand at 2373.606 kb on - strand at 2373.612 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 2,372,769 - -0.0 2,373,422 + -1.8 2,373,429 - +0.1 2,373,429 - -0.6 2,373,447 - -0.1 2,373,474 + -2.5 2,373,485 + -0.2 2,373,493 - +0.1 2,373,545 - -0.4 2,373,604 + +0.6 2,373,606 - +1.0 2,373,612 - -0.3
Or see this region's nucleotide sequence