Experiment: Cytidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2830 and ribA are separated by 42 nucleotides ribA and SO2832 are separated by 262 nucleotides SO2832 and nrdG are separated by 125 nucleotides nrdG and nrdD are separated by 115 nucleotides
SO2830: SO2830 - hypothetical protein (NCBI ptt file), at 2,948,015 to 2,948,953
SO2830
SO2831: ribA - GTP cyclohydrolase II (NCBI ptt file), at 2,948,996 to 2,949,607
ribA
SO2832: SO2832 - conserved hypothetical protein (NCBI ptt file), at 2,949,870 to 2,950,226
SO2832
SO2833: nrdG - anaerobic ribonucleoside-triphosphate reductase activating protein (NCBI ptt file), at 2,950,352 to 2,950,813
nrdG
SO2834: nrdD - anaerobic ribonucleoside-triphosphate reductase (NCBI ptt file), at 2,950,929 to 2,953,046
nrdD
Position (kb)
2949
2950
2951 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2948.920 kb on + strand at 2948.961 kb on - strand at 2949.707 kb on - strand at 2949.790 kb on - strand at 2949.921 kb on + strand, within SO2832 at 2949.921 kb on + strand, within SO2832 at 2949.929 kb on - strand, within SO2832 at 2949.929 kb on - strand, within SO2832 at 2949.978 kb on + strand, within SO2832 at 2949.978 kb on + strand, within SO2832 at 2949.986 kb on - strand, within SO2832 at 2949.986 kb on - strand, within SO2832 at 2949.986 kb on - strand, within SO2832 at 2950.122 kb on - strand, within SO2832 at 2950.122 kb on - strand, within SO2832 at 2950.263 kb on + strand at 2950.263 kb on + strand at 2950.263 kb on + strand at 2950.271 kb on - strand at 2950.271 kb on - strand at 2950.271 kb on - strand at 2950.301 kb on + strand at 2950.317 kb on - strand at 2950.459 kb on - strand, within nrdG at 2950.459 kb on - strand, within nrdG at 2950.541 kb on - strand, within nrdG at 2950.693 kb on + strand, within nrdG at 2950.866 kb on + strand at 2950.866 kb on + strand at 2950.891 kb on + strand at 2950.937 kb on + strand at 2950.974 kb on + strand at 2951.075 kb on - strand at 2951.145 kb on + strand, within nrdD
Per-strain Table
Position Strand Gene LocusTag Fraction Cytidine (C) remove 2,948,920 + +1.3 2,948,961 - -1.1 2,949,707 - +0.2 2,949,790 - -0.5 2,949,921 + SO2832 0.14 +1.1 2,949,921 + SO2832 0.14 +1.1 2,949,929 - SO2832 0.17 +1.4 2,949,929 - SO2832 0.17 +1.3 2,949,978 + SO2832 0.30 +2.1 2,949,978 + SO2832 0.30 +1.0 2,949,986 - SO2832 0.32 +0.4 2,949,986 - SO2832 0.32 +0.1 2,949,986 - SO2832 0.32 +1.6 2,950,122 - SO2832 0.71 +3.0 2,950,122 - SO2832 0.71 +0.2 2,950,263 + +1.4 2,950,263 + -0.3 2,950,263 + +1.0 2,950,271 - +0.7 2,950,271 - +0.6 2,950,271 - -0.3 2,950,301 + +1.3 2,950,317 - +0.6 2,950,459 - nrdG SO2833 0.23 +0.7 2,950,459 - nrdG SO2833 0.23 -0.0 2,950,541 - nrdG SO2833 0.41 -1.0 2,950,693 + nrdG SO2833 0.74 -1.0 2,950,866 + +1.3 2,950,866 + -1.1 2,950,891 + +0.3 2,950,937 + -0.2 2,950,974 + -1.2 2,951,075 - -1.0 2,951,145 + nrdD SO2834 0.10 -0.6
Or see this region's nucleotide sequence