Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1257 and Dshi_1258 are separated by 45 nucleotides Dshi_1258 and Dshi_1259 are separated by 86 nucleotides Dshi_1259 and Dshi_1260 are separated by 10 nucleotides Dshi_1260 and Dshi_1261 are separated by 89 nucleotides
Dshi_1257: Dshi_1257 - hypothetical protein (RefSeq), at 1,303,935 to 1,304,216
_1257
Dshi_1258: Dshi_1258 - short-chain dehydrogenase/reductase SDR (RefSeq), at 1,304,262 to 1,305,005
_1258
Dshi_1259: Dshi_1259 - heavy metal translocating P-type ATPase (RefSeq), at 1,305,092 to 1,307,596
_1259
Dshi_1260: Dshi_1260 - transcriptional regulator, MerR family (RefSeq), at 1,307,607 to 1,308,002
_1260
Dshi_1261: Dshi_1261 - sodium/glutamate symporter (RefSeq), at 1,308,092 to 1,309,300
_1261
Position (kb)
1305
1306
1307
1308 Strain fitness (log2 ratio)
-2
-1
0
1 at 1304.262 kb on - strand at 1304.310 kb on + strand at 1304.320 kb on + strand at 1304.377 kb on + strand, within Dshi_1258 at 1304.523 kb on - strand, within Dshi_1258 at 1304.710 kb on + strand, within Dshi_1258 at 1304.837 kb on + strand, within Dshi_1258 at 1304.852 kb on - strand, within Dshi_1258 at 1304.890 kb on - strand, within Dshi_1258 at 1304.917 kb on - strand, within Dshi_1258 at 1304.985 kb on + strand at 1304.993 kb on - strand at 1305.018 kb on + strand at 1305.094 kb on + strand at 1305.101 kb on + strand at 1305.101 kb on + strand at 1305.180 kb on + strand at 1305.456 kb on + strand at 1305.459 kb on + strand, within Dshi_1259 at 1305.539 kb on - strand, within Dshi_1259 at 1305.829 kb on - strand, within Dshi_1259 at 1305.936 kb on + strand, within Dshi_1259 at 1305.997 kb on + strand at 1306.005 kb on - strand, within Dshi_1259 at 1306.115 kb on - strand, within Dshi_1259 at 1306.218 kb on - strand, within Dshi_1259 at 1306.297 kb on + strand, within Dshi_1259 at 1306.305 kb on - strand, within Dshi_1259 at 1306.410 kb on + strand, within Dshi_1259 at 1306.418 kb on - strand, within Dshi_1259 at 1306.572 kb on - strand, within Dshi_1259 at 1307.064 kb on - strand, within Dshi_1259 at 1307.131 kb on + strand, within Dshi_1259 at 1307.223 kb on + strand, within Dshi_1259 at 1307.252 kb on + strand, within Dshi_1259 at 1307.322 kb on - strand, within Dshi_1259 at 1307.353 kb on + strand at 1307.409 kb on + strand at 1307.653 kb on - strand, within Dshi_1260 at 1307.780 kb on - strand, within Dshi_1260 at 1307.781 kb on + strand, within Dshi_1260 at 1308.051 kb on - strand at 1308.070 kb on - strand at 1308.128 kb on + strand at 1308.133 kb on + strand at 1308.209 kb on - strand at 1308.270 kb on - strand, within Dshi_1261 at 1308.303 kb on + strand, within Dshi_1261 at 1308.418 kb on + strand, within Dshi_1261
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 1,304,262 - -0.9 1,304,310 + -0.8 1,304,320 + +1.4 1,304,377 + Dshi_1258 0.15 -0.5 1,304,523 - Dshi_1258 0.35 -1.0 1,304,710 + Dshi_1258 0.60 -1.0 1,304,837 + Dshi_1258 0.77 -0.2 1,304,852 - Dshi_1258 0.79 -0.1 1,304,890 - Dshi_1258 0.84 +0.3 1,304,917 - Dshi_1258 0.88 -0.3 1,304,985 + +1.3 1,304,993 - -1.5 1,305,018 + +0.1 1,305,094 + -1.1 1,305,101 + -1.6 1,305,101 + -0.5 1,305,180 + -1.0 1,305,456 + +0.3 1,305,459 + Dshi_1259 0.15 +1.5 1,305,539 - Dshi_1259 0.18 +0.6 1,305,829 - Dshi_1259 0.29 +1.1 1,305,936 + Dshi_1259 0.34 -0.7 1,305,997 + +0.2 1,306,005 - Dshi_1259 0.36 -0.8 1,306,115 - Dshi_1259 0.41 -0.8 1,306,218 - Dshi_1259 0.45 +0.3 1,306,297 + Dshi_1259 0.48 -0.3 1,306,305 - Dshi_1259 0.48 -0.7 1,306,410 + Dshi_1259 0.53 +0.1 1,306,418 - Dshi_1259 0.53 -0.0 1,306,572 - Dshi_1259 0.59 +0.1 1,307,064 - Dshi_1259 0.79 +0.6 1,307,131 + Dshi_1259 0.81 +0.0 1,307,223 + Dshi_1259 0.85 -0.1 1,307,252 + Dshi_1259 0.86 -0.3 1,307,322 - Dshi_1259 0.89 +0.8 1,307,353 + +0.3 1,307,409 + +0.7 1,307,653 - Dshi_1260 0.12 +0.3 1,307,780 - Dshi_1260 0.44 +0.4 1,307,781 + Dshi_1260 0.44 +0.6 1,308,051 - -0.4 1,308,070 - -0.6 1,308,128 + -0.2 1,308,133 + +0.4 1,308,209 - +0.9 1,308,270 - Dshi_1261 0.15 -0.3 1,308,303 + Dshi_1261 0.17 +0.7 1,308,418 + Dshi_1261 0.27 -2.5
Or see this region's nucleotide sequence