Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c20680 and PGA1_c20690 are separated by 684 nucleotides PGA1_c20690 and PGA1_c20700 overlap by 4 nucleotides
PGA1_c20680: PGA1_c20680 - TRAP transporter for fumarate, succinate, L-malate, and 2-oxogulatarate, substrate-binding component (from data) , at 2,143,245 to 2,144,246
_c20680
PGA1_c20690: PGA1_c20690 - C4-dicarboxylate transport transcriptional regulatory protein DctD, at 2,144,931 to 2,146,166
_c20690
PGA1_c20700: PGA1_c20700 - C4-dicarboxylate transport sensor protein DctB, at 2,146,163 to 2,147,962
_c20700
Position (kb)
2144
2145
2146
2147 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2143.964 kb on + strand, within PGA1_c20680 at 2143.972 kb on - strand, within PGA1_c20680 at 2143.972 kb on - strand, within PGA1_c20680 at 2144.010 kb on - strand, within PGA1_c20680 at 2144.054 kb on + strand, within PGA1_c20680 at 2144.113 kb on + strand, within PGA1_c20680 at 2144.113 kb on + strand, within PGA1_c20680 at 2144.121 kb on - strand, within PGA1_c20680 at 2144.121 kb on - strand, within PGA1_c20680 at 2144.121 kb on - strand, within PGA1_c20680 at 2144.126 kb on + strand, within PGA1_c20680 at 2144.200 kb on + strand at 2144.292 kb on - strand at 2144.300 kb on - strand at 2144.301 kb on + strand at 2144.323 kb on - strand at 2144.325 kb on + strand at 2144.325 kb on + strand at 2144.333 kb on - strand at 2144.418 kb on + strand at 2144.418 kb on + strand at 2144.426 kb on - strand at 2144.426 kb on - strand at 2144.451 kb on + strand at 2144.451 kb on + strand at 2144.453 kb on - strand at 2144.453 kb on - strand at 2144.459 kb on - strand at 2144.459 kb on - strand at 2144.504 kb on + strand at 2144.562 kb on + strand at 2144.571 kb on - strand at 2144.598 kb on + strand at 2144.627 kb on + strand at 2144.629 kb on - strand at 2144.655 kb on - strand at 2144.655 kb on - strand at 2144.748 kb on - strand at 2144.807 kb on - strand at 2144.844 kb on - strand at 2144.855 kb on - strand at 2144.906 kb on + strand at 2144.937 kb on + strand at 2145.101 kb on + strand, within PGA1_c20690 at 2145.130 kb on - strand, within PGA1_c20690 at 2145.135 kb on - strand, within PGA1_c20690 at 2145.248 kb on + strand, within PGA1_c20690 at 2145.289 kb on - strand, within PGA1_c20690 at 2145.292 kb on + strand, within PGA1_c20690 at 2145.330 kb on + strand, within PGA1_c20690 at 2145.343 kb on - strand, within PGA1_c20690 at 2145.343 kb on - strand, within PGA1_c20690 at 2145.403 kb on + strand, within PGA1_c20690 at 2145.553 kb on - strand, within PGA1_c20690 at 2145.603 kb on + strand, within PGA1_c20690 at 2145.715 kb on - strand, within PGA1_c20690 at 2145.765 kb on - strand, within PGA1_c20690 at 2145.790 kb on + strand, within PGA1_c20690 at 2145.798 kb on - strand, within PGA1_c20690 at 2145.850 kb on + strand, within PGA1_c20690 at 2145.877 kb on - strand, within PGA1_c20690 at 2145.967 kb on - strand, within PGA1_c20690 at 2146.049 kb on - strand at 2146.123 kb on - strand at 2146.184 kb on - strand at 2146.299 kb on - strand at 2146.299 kb on - strand at 2146.310 kb on + strand at 2146.318 kb on - strand at 2146.318 kb on - strand at 2146.318 kb on - strand at 2146.332 kb on - strand at 2146.347 kb on - strand, within PGA1_c20700 at 2146.389 kb on - strand, within PGA1_c20700 at 2146.408 kb on - strand, within PGA1_c20700 at 2146.498 kb on - strand, within PGA1_c20700 at 2146.685 kb on - strand, within PGA1_c20700 at 2146.754 kb on + strand, within PGA1_c20700 at 2146.788 kb on + strand, within PGA1_c20700 at 2146.843 kb on + strand, within PGA1_c20700 at 2146.845 kb on - strand, within PGA1_c20700 at 2146.937 kb on + strand, within PGA1_c20700 at 2146.991 kb on - strand, within PGA1_c20700 at 2147.033 kb on - strand, within PGA1_c20700
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 2,143,964 + PGA1_c20680 0.72 -0.6 2,143,972 - PGA1_c20680 0.73 +0.1 2,143,972 - PGA1_c20680 0.73 -0.5 2,144,010 - PGA1_c20680 0.76 -1.4 2,144,054 + PGA1_c20680 0.81 -2.8 2,144,113 + PGA1_c20680 0.87 -1.9 2,144,113 + PGA1_c20680 0.87 -0.7 2,144,121 - PGA1_c20680 0.87 -1.5 2,144,121 - PGA1_c20680 0.87 -0.7 2,144,121 - PGA1_c20680 0.87 -1.6 2,144,126 + PGA1_c20680 0.88 -1.2 2,144,200 + -0.6 2,144,292 - -1.8 2,144,300 - +0.5 2,144,301 + +0.7 2,144,323 - -0.8 2,144,325 + +1.0 2,144,325 + -0.9 2,144,333 - +0.4 2,144,418 + -1.2 2,144,418 + -1.2 2,144,426 - -1.8 2,144,426 - -3.1 2,144,451 + -0.2 2,144,451 + -2.0 2,144,453 - -3.0 2,144,453 - -0.6 2,144,459 - -2.0 2,144,459 - -2.6 2,144,504 + -0.4 2,144,562 + -0.3 2,144,571 - -0.0 2,144,598 + -1.1 2,144,627 + +0.6 2,144,629 - -0.3 2,144,655 - +0.2 2,144,655 - -0.1 2,144,748 - +1.4 2,144,807 - -1.1 2,144,844 - -0.2 2,144,855 - -0.8 2,144,906 + +1.0 2,144,937 + -0.8 2,145,101 + PGA1_c20690 0.14 -2.4 2,145,130 - PGA1_c20690 0.16 -0.8 2,145,135 - PGA1_c20690 0.17 -1.5 2,145,248 + PGA1_c20690 0.26 -0.5 2,145,289 - PGA1_c20690 0.29 -2.4 2,145,292 + PGA1_c20690 0.29 -0.5 2,145,330 + PGA1_c20690 0.32 -0.3 2,145,343 - PGA1_c20690 0.33 -2.4 2,145,343 - PGA1_c20690 0.33 -2.4 2,145,403 + PGA1_c20690 0.38 -0.0 2,145,553 - PGA1_c20690 0.50 -2.4 2,145,603 + PGA1_c20690 0.54 -1.7 2,145,715 - PGA1_c20690 0.63 -0.8 2,145,765 - PGA1_c20690 0.67 -0.5 2,145,790 + PGA1_c20690 0.69 +0.5 2,145,798 - PGA1_c20690 0.70 -3.7 2,145,850 + PGA1_c20690 0.74 -0.4 2,145,877 - PGA1_c20690 0.77 +0.1 2,145,967 - PGA1_c20690 0.84 -0.9 2,146,049 - -0.7 2,146,123 - -0.7 2,146,184 - +2.8 2,146,299 - -1.2 2,146,299 - -0.2 2,146,310 + -1.6 2,146,318 - +0.6 2,146,318 - -0.3 2,146,318 - -0.6 2,146,332 - -0.2 2,146,347 - PGA1_c20700 0.10 -0.6 2,146,389 - PGA1_c20700 0.13 -1.6 2,146,408 - PGA1_c20700 0.14 -0.6 2,146,498 - PGA1_c20700 0.19 -0.5 2,146,685 - PGA1_c20700 0.29 -0.4 2,146,754 + PGA1_c20700 0.33 -1.4 2,146,788 + PGA1_c20700 0.35 -0.9 2,146,843 + PGA1_c20700 0.38 -0.6 2,146,845 - PGA1_c20700 0.38 -1.4 2,146,937 + PGA1_c20700 0.43 -1.5 2,146,991 - PGA1_c20700 0.46 -2.0 2,147,033 - PGA1_c20700 0.48 -0.5
Or see this region's nucleotide sequence