Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2813 and Dshi_2814 are separated by 12 nucleotides Dshi_2814 and Dshi_2815 overlap by 4 nucleotides Dshi_2815 and Dshi_2816 are separated by 101 nucleotides
Dshi_2813: Dshi_2813 - protein of unknown function DUF395 YeeE/YedE (RefSeq), at 2,968,662 to 2,969,750
_2813
Dshi_2814: Dshi_2814 - diguanylate cyclase (RefSeq), at 2,969,763 to 2,970,803
_2814
Dshi_2815: Dshi_2815 - Heme NO binding domain protein (RefSeq), at 2,970,800 to 2,971,384
_2815
Dshi_2816: Dshi_2816 - hypothetical protein (RefSeq), at 2,971,486 to 2,973,201
_2816
Position (kb)
2969
2970
2971 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2968.843 kb on + strand, within Dshi_2813 at 2968.846 kb on - strand, within Dshi_2813 at 2968.848 kb on + strand, within Dshi_2813 at 2968.914 kb on - strand, within Dshi_2813 at 2969.021 kb on - strand, within Dshi_2813 at 2969.030 kb on - strand, within Dshi_2813 at 2969.102 kb on + strand, within Dshi_2813 at 2969.177 kb on + strand, within Dshi_2813 at 2969.363 kb on + strand at 2969.424 kb on - strand, within Dshi_2813 at 2969.439 kb on + strand, within Dshi_2813 at 2969.439 kb on + strand, within Dshi_2813 at 2969.487 kb on + strand, within Dshi_2813 at 2969.487 kb on + strand, within Dshi_2813 at 2969.487 kb on + strand, within Dshi_2813 at 2969.490 kb on - strand, within Dshi_2813 at 2969.495 kb on - strand, within Dshi_2813 at 2969.495 kb on - strand, within Dshi_2813 at 2969.513 kb on + strand, within Dshi_2813 at 2969.521 kb on - strand, within Dshi_2813 at 2969.521 kb on - strand, within Dshi_2813 at 2969.573 kb on + strand, within Dshi_2813 at 2969.603 kb on + strand, within Dshi_2813 at 2969.661 kb on - strand at 2969.784 kb on + strand at 2969.815 kb on + strand at 2969.846 kb on - strand at 2969.846 kb on - strand at 2969.847 kb on + strand at 2969.847 kb on + strand at 2969.964 kb on + strand, within Dshi_2814 at 2970.104 kb on + strand, within Dshi_2814 at 2970.115 kb on + strand, within Dshi_2814 at 2970.123 kb on - strand, within Dshi_2814 at 2970.282 kb on + strand, within Dshi_2814 at 2970.286 kb on - strand, within Dshi_2814 at 2970.286 kb on - strand, within Dshi_2814 at 2970.318 kb on - strand, within Dshi_2814 at 2970.552 kb on - strand, within Dshi_2814 at 2970.552 kb on - strand, within Dshi_2814 at 2970.607 kb on - strand, within Dshi_2814 at 2970.607 kb on - strand, within Dshi_2814 at 2970.622 kb on + strand, within Dshi_2814 at 2970.768 kb on + strand at 2970.867 kb on - strand, within Dshi_2815 at 2970.871 kb on + strand, within Dshi_2815 at 2970.879 kb on - strand, within Dshi_2815 at 2970.879 kb on - strand, within Dshi_2815 at 2970.984 kb on + strand, within Dshi_2815 at 2971.135 kb on + strand, within Dshi_2815 at 2971.270 kb on - strand, within Dshi_2815 at 2971.348 kb on + strand at 2971.356 kb on - strand at 2971.427 kb on - strand at 2971.490 kb on - strand at 2971.535 kb on - strand at 2971.614 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 2,968,843 + Dshi_2813 0.17 +0.6 2,968,846 - Dshi_2813 0.17 -0.3 2,968,848 + Dshi_2813 0.17 -1.2 2,968,914 - Dshi_2813 0.23 -0.6 2,969,021 - Dshi_2813 0.33 +1.2 2,969,030 - Dshi_2813 0.34 +0.8 2,969,102 + Dshi_2813 0.40 -0.4 2,969,177 + Dshi_2813 0.47 +0.2 2,969,363 + +1.5 2,969,424 - Dshi_2813 0.70 +0.6 2,969,439 + Dshi_2813 0.71 +0.4 2,969,439 + Dshi_2813 0.71 +0.2 2,969,487 + Dshi_2813 0.76 -0.5 2,969,487 + Dshi_2813 0.76 +0.9 2,969,487 + Dshi_2813 0.76 -0.5 2,969,490 - Dshi_2813 0.76 -0.9 2,969,495 - Dshi_2813 0.76 +0.4 2,969,495 - Dshi_2813 0.76 +0.4 2,969,513 + Dshi_2813 0.78 -0.3 2,969,521 - Dshi_2813 0.79 +0.5 2,969,521 - Dshi_2813 0.79 -0.0 2,969,573 + Dshi_2813 0.84 -0.4 2,969,603 + Dshi_2813 0.86 -0.3 2,969,661 - +0.2 2,969,784 + +1.0 2,969,815 + -0.4 2,969,846 - -0.8 2,969,846 - -3.2 2,969,847 + -1.3 2,969,847 + -0.9 2,969,964 + Dshi_2814 0.19 -0.5 2,970,104 + Dshi_2814 0.33 -0.6 2,970,115 + Dshi_2814 0.34 -1.1 2,970,123 - Dshi_2814 0.35 -0.1 2,970,282 + Dshi_2814 0.50 -1.9 2,970,286 - Dshi_2814 0.50 -2.1 2,970,286 - Dshi_2814 0.50 -1.0 2,970,318 - Dshi_2814 0.53 -1.0 2,970,552 - Dshi_2814 0.76 +0.8 2,970,552 - Dshi_2814 0.76 -2.0 2,970,607 - Dshi_2814 0.81 -0.7 2,970,607 - Dshi_2814 0.81 -2.7 2,970,622 + Dshi_2814 0.83 +0.1 2,970,768 + -1.6 2,970,867 - Dshi_2815 0.11 -0.9 2,970,871 + Dshi_2815 0.12 -2.5 2,970,879 - Dshi_2815 0.14 -1.8 2,970,879 - Dshi_2815 0.14 -0.8 2,970,984 + Dshi_2815 0.31 -0.3 2,971,135 + Dshi_2815 0.57 -3.2 2,971,270 - Dshi_2815 0.80 -2.1 2,971,348 + -0.2 2,971,356 - -3.7 2,971,427 - -0.9 2,971,490 - -1.5 2,971,535 - -0.0 2,971,614 + -0.2
Or see this region's nucleotide sequence