Experiment: 36hr Phyto Lib only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31185 and BPHYT_RS31190 are separated by 141 nucleotides BPHYT_RS31190 and BPHYT_RS31195 overlap by 4 nucleotides BPHYT_RS31195 and BPHYT_RS31200 are separated by 8 nucleotides
BPHYT_RS31185: BPHYT_RS31185 - LysR family transcriptional regulator, at 2,554,288 to 2,555,199
_RS31185
BPHYT_RS31190: BPHYT_RS31190 - ABC transporter permease, at 2,555,341 to 2,556,312
_RS31190
BPHYT_RS31195: BPHYT_RS31195 - peptide ABC transporter permease, at 2,556,309 to 2,557,220
_RS31195
BPHYT_RS31200: BPHYT_RS31200 - peptide ABC transporter substrate-binding protein, at 2,557,229 to 2,558,206
_RS31200
Position (kb)
2555
2556
2557 Strain fitness (log2 ratio)
-2
-1
0
1 at 2554.390 kb on + strand, within BPHYT_RS31185 at 2554.390 kb on + strand, within BPHYT_RS31185 at 2554.687 kb on + strand, within BPHYT_RS31185 at 2554.809 kb on - strand, within BPHYT_RS31185 at 2554.809 kb on - strand, within BPHYT_RS31185 at 2554.809 kb on - strand, within BPHYT_RS31185 at 2555.220 kb on - strand at 2555.220 kb on - strand at 2555.220 kb on - strand at 2555.220 kb on - strand at 2555.408 kb on + strand at 2555.530 kb on + strand, within BPHYT_RS31190 at 2555.531 kb on - strand, within BPHYT_RS31190 at 2555.531 kb on - strand, within BPHYT_RS31190 at 2555.670 kb on - strand, within BPHYT_RS31190 at 2555.670 kb on - strand, within BPHYT_RS31190 at 2555.996 kb on - strand, within BPHYT_RS31190 at 2556.271 kb on + strand at 2556.317 kb on + strand at 2556.600 kb on + strand, within BPHYT_RS31195 at 2556.838 kb on - strand, within BPHYT_RS31195 at 2556.888 kb on + strand, within BPHYT_RS31195 at 2556.889 kb on - strand, within BPHYT_RS31195
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib only remove 2,554,390 + BPHYT_RS31185 0.11 -0.6 2,554,390 + BPHYT_RS31185 0.11 -0.6 2,554,687 + BPHYT_RS31185 0.44 -0.4 2,554,809 - BPHYT_RS31185 0.57 +0.0 2,554,809 - BPHYT_RS31185 0.57 -1.8 2,554,809 - BPHYT_RS31185 0.57 -1.1 2,555,220 - -0.4 2,555,220 - -0.7 2,555,220 - -0.2 2,555,220 - +0.8 2,555,408 + -0.1 2,555,530 + BPHYT_RS31190 0.19 +0.3 2,555,531 - BPHYT_RS31190 0.20 -0.4 2,555,531 - BPHYT_RS31190 0.20 +1.5 2,555,670 - BPHYT_RS31190 0.34 -0.3 2,555,670 - BPHYT_RS31190 0.34 +1.1 2,555,996 - BPHYT_RS31190 0.67 -1.0 2,556,271 + -0.4 2,556,317 + -0.8 2,556,600 + BPHYT_RS31195 0.32 -1.3 2,556,838 - BPHYT_RS31195 0.58 -0.8 2,556,888 + BPHYT_RS31195 0.63 +0.1 2,556,889 - BPHYT_RS31195 0.64 -0.8
Or see this region's nucleotide sequence