Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25365

Experiment: 36hr Phyto Lib only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25355 and BPHYT_RS25360 are separated by 184 nucleotidesBPHYT_RS25360 and BPHYT_RS25365 are separated by 27 nucleotidesBPHYT_RS25365 and BPHYT_RS25370 are separated by 26 nucleotides BPHYT_RS25355: BPHYT_RS25355 - LuxR family transcriptional regulator, at 1,264,324 to 1,267,026 _RS25355 BPHYT_RS25360: BPHYT_RS25360 - enoyl-CoA hydratase, at 1,267,211 to 1,267,981 _RS25360 BPHYT_RS25365: BPHYT_RS25365 - thiolase, at 1,268,009 to 1,269,250 _RS25365 BPHYT_RS25370: BPHYT_RS25370 - acyl-CoA dehydrogenase, at 1,269,277 to 1,270,419 _RS25370 Position (kb) 1268 1269 1270Strain fitness (log2 ratio) -1 0 1at 1267.020 kb on + strandat 1267.098 kb on + strandat 1267.098 kb on + strandat 1267.099 kb on - strandat 1267.917 kb on - strandat 1267.917 kb on - strandat 1268.021 kb on + strandat 1268.022 kb on - strandat 1269.270 kb on - strandat 1269.274 kb on - strandat 1269.612 kb on + strand, within BPHYT_RS25370at 1269.613 kb on - strand, within BPHYT_RS25370

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib only
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1,267,020 + -0.8
1,267,098 + +0.1
1,267,098 + +0.2
1,267,099 - -0.6
1,267,917 - -0.8
1,267,917 - +0.0
1,268,021 + -0.2
1,268,022 - +0.9
1,269,270 - -0.9
1,269,274 - +0.5
1,269,612 + BPHYT_RS25370 0.29 -1.5
1,269,613 - BPHYT_RS25370 0.29 -0.9

Or see this region's nucleotide sequence