Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25355

Experiment: 36hr Phyto Lib only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25350 and BPHYT_RS25355 are separated by 91 nucleotidesBPHYT_RS25355 and BPHYT_RS25360 are separated by 184 nucleotidesBPHYT_RS25360 and BPHYT_RS25365 are separated by 27 nucleotides BPHYT_RS25350: BPHYT_RS25350 - AMP-dependent synthetase and ligase, at 1,262,532 to 1,264,232 _RS25350 BPHYT_RS25355: BPHYT_RS25355 - LuxR family transcriptional regulator, at 1,264,324 to 1,267,026 _RS25355 BPHYT_RS25360: BPHYT_RS25360 - enoyl-CoA hydratase, at 1,267,211 to 1,267,981 _RS25360 BPHYT_RS25365: BPHYT_RS25365 - thiolase, at 1,268,009 to 1,269,250 _RS25365 Position (kb) 1264 1265 1266 1267 1268Strain fitness (log2 ratio) -2 -1 0 1at 1263.613 kb on - strand, within BPHYT_RS25350at 1264.156 kb on - strandat 1264.201 kb on + strandat 1264.610 kb on + strand, within BPHYT_RS25355at 1264.610 kb on + strand, within BPHYT_RS25355at 1264.611 kb on - strand, within BPHYT_RS25355at 1265.185 kb on + strand, within BPHYT_RS25355at 1265.392 kb on + strand, within BPHYT_RS25355at 1265.393 kb on - strand, within BPHYT_RS25355at 1265.832 kb on - strand, within BPHYT_RS25355at 1266.782 kb on + strandat 1266.782 kb on + strandat 1267.020 kb on + strandat 1267.098 kb on + strandat 1267.098 kb on + strandat 1267.099 kb on - strandat 1267.917 kb on - strandat 1267.917 kb on - strandat 1268.021 kb on + strandat 1268.022 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib only
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1,263,613 - BPHYT_RS25350 0.64 -0.7
1,264,156 - +0.7
1,264,201 + -0.3
1,264,610 + BPHYT_RS25355 0.11 -0.3
1,264,610 + BPHYT_RS25355 0.11 +0.8
1,264,611 - BPHYT_RS25355 0.11 -0.4
1,265,185 + BPHYT_RS25355 0.32 +0.5
1,265,392 + BPHYT_RS25355 0.40 -1.8
1,265,393 - BPHYT_RS25355 0.40 -0.4
1,265,832 - BPHYT_RS25355 0.56 -0.3
1,266,782 + +0.1
1,266,782 + -0.6
1,267,020 + -0.8
1,267,098 + +0.1
1,267,098 + +0.2
1,267,099 - -0.6
1,267,917 - -0.8
1,267,917 - +0.0
1,268,021 + -0.2
1,268,022 - +0.9

Or see this region's nucleotide sequence