Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25165

Experiment: 36hr Phyto Lib only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25160 and BPHYT_RS25165 are separated by 42 nucleotidesBPHYT_RS25165 and BPHYT_RS25170 are separated by 283 nucleotides BPHYT_RS25160: BPHYT_RS25160 - NAD/NADP-dependent betaine aldehyde dehydrogenase, at 1,216,722 to 1,218,191 _RS25160 BPHYT_RS25165: BPHYT_RS25165 - HTH-type transcriptional regulator BetI, at 1,218,234 to 1,218,821 _RS25165 BPHYT_RS25170: BPHYT_RS25170 - aldehyde dismutase, at 1,219,105 to 1,220,304 _RS25170 Position (kb) 1218 1219Strain fitness (log2 ratio) -2 -1 0 1at 1217.845 kb on + strand, within BPHYT_RS25160at 1218.164 kb on - strandat 1218.164 kb on - strandat 1218.264 kb on - strandat 1218.446 kb on - strand, within BPHYT_RS25165at 1218.446 kb on - strand, within BPHYT_RS25165at 1218.834 kb on - strandat 1218.836 kb on - strandat 1219.129 kb on + strandat 1219.130 kb on - strandat 1219.429 kb on - strand, within BPHYT_RS25170at 1219.475 kb on - strand, within BPHYT_RS25170at 1219.676 kb on - strand, within BPHYT_RS25170

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib only
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1,217,845 + BPHYT_RS25160 0.76 +0.5
1,218,164 - +0.2
1,218,164 - +0.4
1,218,264 - -0.5
1,218,446 - BPHYT_RS25165 0.36 -1.0
1,218,446 - BPHYT_RS25165 0.36 -2.4
1,218,834 - +0.2
1,218,836 - +0.7
1,219,129 + -0.1
1,219,130 - +0.4
1,219,429 - BPHYT_RS25170 0.27 +0.9
1,219,475 - BPHYT_RS25170 0.31 +0.9
1,219,676 - BPHYT_RS25170 0.48 +0.6

Or see this region's nucleotide sequence