Experiment: 36hr Phyto Lib only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21145 and BPHYT_RS21150 are separated by 67 nucleotides BPHYT_RS21150 and BPHYT_RS21155 are separated by 10 nucleotides BPHYT_RS21155 and BPHYT_RS21160 overlap by 4 nucleotides
BPHYT_RS21145: BPHYT_RS21145 - allantoate amidohydrolase, at 304,405 to 305,643
_RS21145
BPHYT_RS21150: BPHYT_RS21150 - NAD(P) transhydrogenase subunit alpha, at 305,711 to 306,829
_RS21150
BPHYT_RS21155: BPHYT_RS21155 - NAD(P) transhydrogenase, at 306,840 to 307,196
_RS21155
BPHYT_RS21160: BPHYT_RS21160 - NAD(P) transhydrogenase subunit beta, at 307,193 to 308,656
_RS21160
Position (kb) 305
306
307 Strain fitness (log2 ratio) -1
0
1 at 304.773 kb on + strand, within BPHYT_RS21145 at 305.724 kb on + strand at 305.786 kb on + strand at 305.858 kb on + strand, within BPHYT_RS21150 at 305.859 kb on - strand, within BPHYT_RS21150 at 306.122 kb on + strand, within BPHYT_RS21150 at 306.123 kb on - strand, within BPHYT_RS21150 at 306.699 kb on - strand, within BPHYT_RS21150 at 306.847 kb on + strand at 306.883 kb on + strand, within BPHYT_RS21155 at 306.894 kb on + strand, within BPHYT_RS21155 at 307.229 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib only remove 304,773 + BPHYT_RS21145 0.30 +1.1 305,724 + +1.7 305,786 + +0.9 305,858 + BPHYT_RS21150 0.13 +0.7 305,859 - BPHYT_RS21150 0.13 -1.3 306,122 + BPHYT_RS21150 0.37 +0.6 306,123 - BPHYT_RS21150 0.37 -0.1 306,699 - BPHYT_RS21150 0.88 +0.9 306,847 + -0.4 306,883 + BPHYT_RS21155 0.12 -0.7 306,894 + BPHYT_RS21155 0.15 -0.4 307,229 + -0.2
Or see this region's nucleotide sequence