Experiment: 36hr Phyto Lib only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17860 and BPHYT_RS17865 are separated by 187 nucleotides BPHYT_RS17865 and BPHYT_RS17870 overlap by 4 nucleotides BPHYT_RS17870 and BPHYT_RS17875 are separated by 80 nucleotides BPHYT_RS17875 and BPHYT_RS17880 are separated by 125 nucleotides
BPHYT_RS17860: BPHYT_RS17860 - transposase, at 4,034,822 to 4,035,529
_RS17860
BPHYT_RS17865: BPHYT_RS17865 - membrane protein, at 4,035,717 to 4,036,442
_RS17865
BPHYT_RS17870: BPHYT_RS17870 - hypothetical protein, at 4,036,439 to 4,036,711
_RS17870
BPHYT_RS17875: BPHYT_RS17875 - hypothetical protein, at 4,036,792 to 4,037,172
_RS17875
BPHYT_RS17880: BPHYT_RS17880 - deoxyguanosinetriphosphate triphosphohydrolase, at 4,037,298 to 4,038,503
_RS17880
Position (kb)
4035
4036
4037 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4034.917 kb on - strand, within BPHYT_RS17860 at 4035.120 kb on + strand, within BPHYT_RS17860 at 4035.121 kb on - strand, within BPHYT_RS17860 at 4035.192 kb on + strand, within BPHYT_RS17860 at 4035.215 kb on - strand, within BPHYT_RS17860 at 4035.280 kb on - strand, within BPHYT_RS17860 at 4035.280 kb on - strand, within BPHYT_RS17860 at 4035.432 kb on + strand, within BPHYT_RS17860 at 4035.507 kb on + strand at 4035.507 kb on + strand at 4035.507 kb on + strand at 4035.507 kb on + strand at 4035.507 kb on + strand at 4035.507 kb on + strand at 4035.507 kb on + strand at 4035.507 kb on + strand at 4035.507 kb on + strand at 4035.556 kb on + strand at 4035.557 kb on - strand at 4035.557 kb on - strand at 4035.557 kb on - strand at 4035.557 kb on - strand at 4035.660 kb on - strand at 4035.680 kb on + strand at 4035.680 kb on + strand at 4035.687 kb on - strand at 4035.716 kb on + strand at 4035.801 kb on + strand, within BPHYT_RS17865 at 4035.801 kb on + strand, within BPHYT_RS17865 at 4035.827 kb on - strand, within BPHYT_RS17865 at 4036.089 kb on + strand, within BPHYT_RS17865 at 4036.093 kb on - strand, within BPHYT_RS17865 at 4036.220 kb on + strand, within BPHYT_RS17865 at 4036.221 kb on - strand, within BPHYT_RS17865 at 4036.223 kb on + strand, within BPHYT_RS17865 at 4036.224 kb on - strand, within BPHYT_RS17865 at 4036.229 kb on + strand, within BPHYT_RS17865 at 4036.230 kb on - strand, within BPHYT_RS17865 at 4036.266 kb on + strand, within BPHYT_RS17865 at 4036.267 kb on - strand, within BPHYT_RS17865 at 4036.267 kb on - strand, within BPHYT_RS17865 at 4036.297 kb on + strand, within BPHYT_RS17865 at 4036.308 kb on + strand, within BPHYT_RS17865 at 4036.416 kb on + strand at 4036.471 kb on + strand, within BPHYT_RS17870 at 4036.471 kb on + strand, within BPHYT_RS17870 at 4036.471 kb on + strand, within BPHYT_RS17870 at 4036.471 kb on + strand, within BPHYT_RS17870 at 4036.472 kb on - strand, within BPHYT_RS17870 at 4036.472 kb on - strand, within BPHYT_RS17870 at 4036.477 kb on - strand, within BPHYT_RS17870 at 4036.637 kb on + strand, within BPHYT_RS17870 at 4036.637 kb on + strand, within BPHYT_RS17870 at 4036.737 kb on - strand at 4036.810 kb on - strand at 4036.863 kb on + strand, within BPHYT_RS17875 at 4036.897 kb on - strand, within BPHYT_RS17875 at 4036.919 kb on + strand, within BPHYT_RS17875 at 4036.920 kb on - strand, within BPHYT_RS17875 at 4036.992 kb on + strand, within BPHYT_RS17875 at 4036.993 kb on - strand, within BPHYT_RS17875 at 4037.083 kb on + strand, within BPHYT_RS17875 at 4037.083 kb on + strand, within BPHYT_RS17875 at 4037.084 kb on - strand, within BPHYT_RS17875 at 4037.193 kb on - strand at 4037.241 kb on - strand at 4037.242 kb on + strand at 4037.243 kb on - strand at 4037.243 kb on - strand at 4037.329 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib only remove 4,034,917 - BPHYT_RS17860 0.13 +2.1 4,035,120 + BPHYT_RS17860 0.42 -0.3 4,035,121 - BPHYT_RS17860 0.42 +1.0 4,035,192 + BPHYT_RS17860 0.52 -0.0 4,035,215 - BPHYT_RS17860 0.56 +0.2 4,035,280 - BPHYT_RS17860 0.65 +1.0 4,035,280 - BPHYT_RS17860 0.65 +2.4 4,035,432 + BPHYT_RS17860 0.86 -1.4 4,035,507 + -1.6 4,035,507 + -0.6 4,035,507 + +1.0 4,035,507 + -0.6 4,035,507 + +0.0 4,035,507 + -2.1 4,035,507 + -2.8 4,035,507 + +2.0 4,035,507 + -1.2 4,035,556 + +0.0 4,035,557 - -1.0 4,035,557 - -0.5 4,035,557 - -0.5 4,035,557 - -0.1 4,035,660 - -1.0 4,035,680 + -0.8 4,035,680 + +2.5 4,035,687 - +0.5 4,035,716 + +0.2 4,035,801 + BPHYT_RS17865 0.12 +0.3 4,035,801 + BPHYT_RS17865 0.12 -1.3 4,035,827 - BPHYT_RS17865 0.15 +2.5 4,036,089 + BPHYT_RS17865 0.51 -0.4 4,036,093 - BPHYT_RS17865 0.52 -0.1 4,036,220 + BPHYT_RS17865 0.69 +0.3 4,036,221 - BPHYT_RS17865 0.69 +0.5 4,036,223 + BPHYT_RS17865 0.70 +1.0 4,036,224 - BPHYT_RS17865 0.70 +2.1 4,036,229 + BPHYT_RS17865 0.71 -2.0 4,036,230 - BPHYT_RS17865 0.71 +1.0 4,036,266 + BPHYT_RS17865 0.76 +0.6 4,036,267 - BPHYT_RS17865 0.76 +2.5 4,036,267 - BPHYT_RS17865 0.76 +1.0 4,036,297 + BPHYT_RS17865 0.80 +1.1 4,036,308 + BPHYT_RS17865 0.81 +1.9 4,036,416 + +0.6 4,036,471 + BPHYT_RS17870 0.12 -1.7 4,036,471 + BPHYT_RS17870 0.12 +2.1 4,036,471 + BPHYT_RS17870 0.12 +0.6 4,036,471 + BPHYT_RS17870 0.12 +0.0 4,036,472 - BPHYT_RS17870 0.12 -0.5 4,036,472 - BPHYT_RS17870 0.12 +1.5 4,036,477 - BPHYT_RS17870 0.14 +2.5 4,036,637 + BPHYT_RS17870 0.73 -0.8 4,036,637 + BPHYT_RS17870 0.73 +0.1 4,036,737 - +2.5 4,036,810 - +1.0 4,036,863 + BPHYT_RS17875 0.19 +0.4 4,036,897 - BPHYT_RS17875 0.28 +1.6 4,036,919 + BPHYT_RS17875 0.33 -0.1 4,036,920 - BPHYT_RS17875 0.34 -0.4 4,036,992 + BPHYT_RS17875 0.52 +0.2 4,036,993 - BPHYT_RS17875 0.53 +1.6 4,037,083 + BPHYT_RS17875 0.76 -0.8 4,037,083 + BPHYT_RS17875 0.76 -0.3 4,037,084 - BPHYT_RS17875 0.77 -0.6 4,037,193 - -0.8 4,037,241 - -1.1 4,037,242 + -1.5 4,037,243 - -0.3 4,037,243 - -0.4 4,037,329 + -0.0
Or see this region's nucleotide sequence