Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS17025

Experiment: 36hr Phyto Lib only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS17015 and BPHYT_RS17020 are separated by 3 nucleotidesBPHYT_RS17020 and BPHYT_RS17025 are separated by 48 nucleotidesBPHYT_RS17025 and BPHYT_RS17030 are separated by 73 nucleotidesBPHYT_RS17030 and BPHYT_RS17035 are separated by 94 nucleotides BPHYT_RS17015: BPHYT_RS17015 - acetyl-CoA carboxylase biotin carboxylase subunit, at 3,857,221 to 3,858,588 _RS17015 BPHYT_RS17020: BPHYT_RS17020 - ribosomal protein L11 methyltransferase, at 3,858,592 to 3,859,494 _RS17020 BPHYT_RS17025: BPHYT_RS17025 - hypothetical protein, at 3,859,543 to 3,860,943 _RS17025 BPHYT_RS17030: BPHYT_RS17030 - lipid hydroperoxide peroxidase, at 3,861,017 to 3,861,517 _RS17030 BPHYT_RS17035: BPHYT_RS17035 - sugar kinase, at 3,861,612 to 3,862,550 _RS17035 Position (kb) 3859 3860 3861Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 3859.044 kb on + strand, within BPHYT_RS17020at 3859.212 kb on + strand, within BPHYT_RS17020at 3859.240 kb on + strand, within BPHYT_RS17020at 3859.241 kb on - strand, within BPHYT_RS17020at 3859.241 kb on - strand, within BPHYT_RS17020at 3859.241 kb on - strand, within BPHYT_RS17020at 3859.290 kb on + strand, within BPHYT_RS17020at 3859.293 kb on + strand, within BPHYT_RS17020at 3859.293 kb on + strand, within BPHYT_RS17020at 3859.293 kb on + strand, within BPHYT_RS17020at 3859.294 kb on - strand, within BPHYT_RS17020at 3859.294 kb on - strand, within BPHYT_RS17020at 3859.412 kb on - strandat 3859.412 kb on - strandat 3859.437 kb on - strandat 3859.493 kb on - strandat 3859.493 kb on - strandat 3859.493 kb on - strandat 3859.504 kb on + strandat 3859.504 kb on + strandat 3859.505 kb on - strandat 3859.505 kb on - strandat 3859.542 kb on + strandat 3859.542 kb on + strandat 3859.542 kb on + strandat 3859.649 kb on + strandat 3859.733 kb on + strand, within BPHYT_RS17025at 3860.002 kb on + strand, within BPHYT_RS17025at 3860.003 kb on - strand, within BPHYT_RS17025at 3860.028 kb on + strand, within BPHYT_RS17025at 3860.128 kb on + strand, within BPHYT_RS17025at 3860.563 kb on + strand, within BPHYT_RS17025at 3860.564 kb on - strand, within BPHYT_RS17025at 3860.564 kb on - strand, within BPHYT_RS17025at 3860.587 kb on + strand, within BPHYT_RS17025at 3860.587 kb on + strand, within BPHYT_RS17025at 3860.818 kb on + strandat 3860.941 kb on + strandat 3861.054 kb on + strandat 3861.365 kb on + strand, within BPHYT_RS17030at 3861.515 kb on + strandat 3861.515 kb on + strandat 3861.596 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib only
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3,859,044 + BPHYT_RS17020 0.50 -0.7
3,859,212 + BPHYT_RS17020 0.69 +0.2
3,859,240 + BPHYT_RS17020 0.72 -0.5
3,859,241 - BPHYT_RS17020 0.72 -0.7
3,859,241 - BPHYT_RS17020 0.72 +0.2
3,859,241 - BPHYT_RS17020 0.72 +0.9
3,859,290 + BPHYT_RS17020 0.77 -2.2
3,859,293 + BPHYT_RS17020 0.78 -0.1
3,859,293 + BPHYT_RS17020 0.78 -0.1
3,859,293 + BPHYT_RS17020 0.78 -0.9
3,859,294 - BPHYT_RS17020 0.78 -1.7
3,859,294 - BPHYT_RS17020 0.78 -1.3
3,859,412 - -2.2
3,859,412 - -1.4
3,859,437 - +4.9
3,859,493 - -0.2
3,859,493 - -0.2
3,859,493 - +0.8
3,859,504 + -0.5
3,859,504 + -0.1
3,859,505 - +0.2
3,859,505 - +2.5
3,859,542 + -1.0
3,859,542 + +0.4
3,859,542 + +3.5
3,859,649 + +0.5
3,859,733 + BPHYT_RS17025 0.14 -0.8
3,860,002 + BPHYT_RS17025 0.33 -0.4
3,860,003 - BPHYT_RS17025 0.33 +3.5
3,860,028 + BPHYT_RS17025 0.35 -0.3
3,860,128 + BPHYT_RS17025 0.42 -1.0
3,860,563 + BPHYT_RS17025 0.73 -0.0
3,860,564 - BPHYT_RS17025 0.73 +2.5
3,860,564 - BPHYT_RS17025 0.73 -0.9
3,860,587 + BPHYT_RS17025 0.75 +0.2
3,860,587 + BPHYT_RS17025 0.75 -0.9
3,860,818 + -0.4
3,860,941 + +0.1
3,861,054 + -0.2
3,861,365 + BPHYT_RS17030 0.69 -1.7
3,861,515 + +1.3
3,861,515 + +0.0
3,861,596 + +0.1

Or see this region's nucleotide sequence