Experiment: 36hr Phyto Lib only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16115 and BPHYT_RS16120 are separated by 72 nucleotides BPHYT_RS16120 and BPHYT_RS16125 are separated by 332 nucleotides BPHYT_RS16125 and BPHYT_RS16130 are separated by 59 nucleotides
BPHYT_RS16115: BPHYT_RS16115 - ABC transporter for D-Sorbitol, periplasmic substrate-binding component (from data) , at 3,655,894 to 3,657,216
_RS16115
BPHYT_RS16120: BPHYT_RS16120 - sorbitol dehydrogenase, D-fructose forming (EC 1.1.1.14) (from data) , at 3,657,289 to 3,658,071
_RS16120
BPHYT_RS16125: BPHYT_RS16125 - Fur family transcriptional regulator, at 3,658,404 to 3,658,898
_RS16125
BPHYT_RS16130: BPHYT_RS16130 - cation ABC transporter substrate-binding protein, at 3,658,958 to 3,659,926
_RS16130
Position (kb)
3657
3658
3659 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3656.400 kb on - strand, within BPHYT_RS16115 at 3656.515 kb on + strand, within BPHYT_RS16115 at 3656.528 kb on + strand, within BPHYT_RS16115 at 3656.529 kb on - strand, within BPHYT_RS16115 at 3656.529 kb on - strand, within BPHYT_RS16115 at 3656.627 kb on + strand at 3656.675 kb on + strand, within BPHYT_RS16115 at 3656.730 kb on - strand, within BPHYT_RS16115 at 3656.786 kb on + strand, within BPHYT_RS16115 at 3656.786 kb on + strand, within BPHYT_RS16115 at 3656.786 kb on + strand, within BPHYT_RS16115 at 3656.787 kb on - strand, within BPHYT_RS16115 at 3656.807 kb on + strand, within BPHYT_RS16115 at 3656.808 kb on - strand, within BPHYT_RS16115 at 3656.808 kb on - strand, within BPHYT_RS16115 at 3656.822 kb on + strand, within BPHYT_RS16115 at 3656.822 kb on + strand, within BPHYT_RS16115 at 3657.240 kb on + strand at 3657.244 kb on + strand at 3657.244 kb on + strand at 3657.244 kb on + strand at 3657.461 kb on + strand, within BPHYT_RS16120 at 3657.461 kb on + strand, within BPHYT_RS16120 at 3657.501 kb on + strand, within BPHYT_RS16120 at 3657.576 kb on - strand, within BPHYT_RS16120 at 3657.651 kb on + strand, within BPHYT_RS16120 at 3657.651 kb on + strand, within BPHYT_RS16120 at 3657.652 kb on - strand, within BPHYT_RS16120 at 3657.915 kb on - strand, within BPHYT_RS16120 at 3657.915 kb on - strand, within BPHYT_RS16120 at 3657.993 kb on - strand at 3658.253 kb on + strand at 3658.253 kb on + strand at 3658.254 kb on - strand at 3658.254 kb on - strand at 3658.254 kb on - strand at 3658.254 kb on - strand at 3658.257 kb on - strand at 3658.322 kb on - strand at 3658.507 kb on - strand, within BPHYT_RS16125 at 3658.507 kb on - strand, within BPHYT_RS16125 at 3658.545 kb on + strand, within BPHYT_RS16125 at 3658.546 kb on - strand, within BPHYT_RS16125 at 3658.600 kb on + strand, within BPHYT_RS16125 at 3658.600 kb on + strand, within BPHYT_RS16125 at 3658.600 kb on + strand, within BPHYT_RS16125 at 3658.600 kb on + strand, within BPHYT_RS16125 at 3658.600 kb on + strand, within BPHYT_RS16125 at 3658.914 kb on + strand at 3658.914 kb on + strand at 3658.914 kb on + strand at 3658.914 kb on + strand at 3658.915 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib only remove 3,656,400 - BPHYT_RS16115 0.38 +2.1 3,656,515 + BPHYT_RS16115 0.47 -0.8 3,656,528 + BPHYT_RS16115 0.48 -1.5 3,656,529 - BPHYT_RS16115 0.48 -1.7 3,656,529 - BPHYT_RS16115 0.48 +1.1 3,656,627 + +2.2 3,656,675 + BPHYT_RS16115 0.59 -0.2 3,656,730 - BPHYT_RS16115 0.63 -0.6 3,656,786 + BPHYT_RS16115 0.67 -0.6 3,656,786 + BPHYT_RS16115 0.67 -1.2 3,656,786 + BPHYT_RS16115 0.67 +0.4 3,656,787 - BPHYT_RS16115 0.67 +2.5 3,656,807 + BPHYT_RS16115 0.69 -1.0 3,656,808 - BPHYT_RS16115 0.69 +0.6 3,656,808 - BPHYT_RS16115 0.69 -0.5 3,656,822 + BPHYT_RS16115 0.70 -0.2 3,656,822 + BPHYT_RS16115 0.70 -0.7 3,657,240 + +0.2 3,657,244 + -1.5 3,657,244 + -0.6 3,657,244 + -0.1 3,657,461 + BPHYT_RS16120 0.22 -1.0 3,657,461 + BPHYT_RS16120 0.22 +0.5 3,657,501 + BPHYT_RS16120 0.27 +1.3 3,657,576 - BPHYT_RS16120 0.37 -0.2 3,657,651 + BPHYT_RS16120 0.46 -0.0 3,657,651 + BPHYT_RS16120 0.46 +4.1 3,657,652 - BPHYT_RS16120 0.46 +2.5 3,657,915 - BPHYT_RS16120 0.80 -0.8 3,657,915 - BPHYT_RS16120 0.80 -0.6 3,657,993 - +0.5 3,658,253 + +0.1 3,658,253 + -0.5 3,658,254 - -1.5 3,658,254 - -1.0 3,658,254 - -0.4 3,658,254 - -0.6 3,658,257 - +0.2 3,658,322 - +1.4 3,658,507 - BPHYT_RS16125 0.21 -0.5 3,658,507 - BPHYT_RS16125 0.21 -1.0 3,658,545 + BPHYT_RS16125 0.28 -0.5 3,658,546 - BPHYT_RS16125 0.29 +0.6 3,658,600 + BPHYT_RS16125 0.40 +1.5 3,658,600 + BPHYT_RS16125 0.40 -3.0 3,658,600 + BPHYT_RS16125 0.40 -0.3 3,658,600 + BPHYT_RS16125 0.40 -1.2 3,658,600 + BPHYT_RS16125 0.40 -0.2 3,658,914 + -0.1 3,658,914 + -0.6 3,658,914 + -0.5 3,658,914 + -0.6 3,658,915 - -1.0
Or see this region's nucleotide sequence