Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10035

Experiment: 36hr Phyto Lib only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10030 and BPHYT_RS10035 are separated by 82 nucleotidesBPHYT_RS10035 and BPHYT_RS10040 are separated by 359 nucleotidesBPHYT_RS10040 and BPHYT_RS10045 are separated by 131 nucleotides BPHYT_RS10030: BPHYT_RS10030 - hemolysin D, at 2,283,831 to 2,285,072 _RS10030 BPHYT_RS10035: BPHYT_RS10035 - DSBA oxidoreductase, at 2,285,155 to 2,286,717 _RS10035 BPHYT_RS10040: BPHYT_RS10040 - hypothetical protein, at 2,287,077 to 2,287,283 _RS10040 BPHYT_RS10045: BPHYT_RS10045 - phosphohydrolase, at 2,287,415 to 2,288,110 _RS10045 Position (kb) 2285 2286 2287Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2284.204 kb on - strand, within BPHYT_RS10030at 2284.486 kb on - strand, within BPHYT_RS10030at 2284.684 kb on - strand, within BPHYT_RS10030at 2284.948 kb on + strandat 2285.665 kb on + strand, within BPHYT_RS10035at 2285.737 kb on + strand, within BPHYT_RS10035at 2286.013 kb on + strand, within BPHYT_RS10035at 2286.065 kb on - strand, within BPHYT_RS10035at 2286.525 kb on + strand, within BPHYT_RS10035at 2286.859 kb on + strandat 2286.944 kb on + strandat 2286.944 kb on + strandat 2286.945 kb on - strandat 2286.945 kb on - strandat 2287.070 kb on + strandat 2287.206 kb on + strand, within BPHYT_RS10040at 2287.416 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib only
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2,284,204 - BPHYT_RS10030 0.30 +1.0
2,284,486 - BPHYT_RS10030 0.53 -1.9
2,284,684 - BPHYT_RS10030 0.69 -0.2
2,284,948 + -0.2
2,285,665 + BPHYT_RS10035 0.33 +1.0
2,285,737 + BPHYT_RS10035 0.37 +1.7
2,286,013 + BPHYT_RS10035 0.55 -0.5
2,286,065 - BPHYT_RS10035 0.58 -3.0
2,286,525 + BPHYT_RS10035 0.88 -0.9
2,286,859 + -0.8
2,286,944 + +2.6
2,286,944 + -1.3
2,286,945 - +0.0
2,286,945 - -0.8
2,287,070 + +0.6
2,287,206 + BPHYT_RS10040 0.62 -1.4
2,287,416 + +0.8

Or see this region's nucleotide sequence