Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09565

Experiment: 36hr Phyto Lib only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09560 and BPHYT_RS09565 are separated by 88 nucleotidesBPHYT_RS09565 and BPHYT_RS09570 are separated by 117 nucleotidesBPHYT_RS09570 and BPHYT_RS09575 are separated by 70 nucleotides BPHYT_RS09560: BPHYT_RS09560 - 3-hydroxyacyl-CoA dehydrogenase, at 2,169,035 to 2,171,119 _RS09560 BPHYT_RS09565: BPHYT_RS09565 - acetyl-CoA acetyltransferase, at 2,171,208 to 2,172,386 _RS09565 BPHYT_RS09570: BPHYT_RS09570 - Water stress and Hypersensitive response domain-containing protein, at 2,172,504 to 2,173,007 _RS09570 BPHYT_RS09575: BPHYT_RS09575 - hypothetical protein, at 2,173,078 to 2,173,452 _RS09575 Position (kb) 2171 2172 2173Strain fitness (log2 ratio) -2 -1 0 1at 2170.490 kb on + strand, within BPHYT_RS09560at 2170.491 kb on - strand, within BPHYT_RS09560at 2170.938 kb on - strandat 2170.965 kb on - strandat 2171.122 kb on + strandat 2171.123 kb on - strandat 2171.195 kb on + strandat 2172.098 kb on + strand, within BPHYT_RS09565at 2172.181 kb on - strand, within BPHYT_RS09565at 2172.959 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib only
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2,170,490 + BPHYT_RS09560 0.70 -2.3
2,170,491 - BPHYT_RS09560 0.70 -1.8
2,170,938 - +0.3
2,170,965 - -1.0
2,171,122 + -0.4
2,171,123 - +0.5
2,171,195 + -0.4
2,172,098 + BPHYT_RS09565 0.75 -2.3
2,172,181 - BPHYT_RS09565 0.83 +1.1
2,172,959 + -1.0

Or see this region's nucleotide sequence