Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27255

Experiment: 36hr Phyto Lib only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27245 and BPHYT_RS27250 are separated by 154 nucleotidesBPHYT_RS27250 and BPHYT_RS27255 are separated by 102 nucleotidesBPHYT_RS27255 and BPHYT_RS27260 are separated by 274 nucleotides BPHYT_RS27245: BPHYT_RS27245 - hypothetical protein, at 1,688,483 to 1,688,725 _RS27245 BPHYT_RS27250: BPHYT_RS27250 - NAD-dependent epimerase/dehydratase, at 1,688,880 to 1,689,518 _RS27250 BPHYT_RS27255: BPHYT_RS27255 - hypothetical protein, at 1,689,621 to 1,689,800 _RS27255 BPHYT_RS27260: BPHYT_RS27260 - membrane protein, at 1,690,075 to 1,690,605 _RS27260 Position (kb) 1689 1690Strain fitness (log2 ratio) -1 0 1at 1689.145 kb on + strand, within BPHYT_RS27250at 1689.320 kb on + strand, within BPHYT_RS27250at 1689.838 kb on - strandat 1690.156 kb on + strand, within BPHYT_RS27260at 1690.748 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib only
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1,689,145 + BPHYT_RS27250 0.41 +0.3
1,689,320 + BPHYT_RS27250 0.69 -1.3
1,689,838 - -1.4
1,690,156 + BPHYT_RS27260 0.15 +0.3
1,690,748 - +0.5

Or see this region's nucleotide sequence