Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25375

Experiment: 36hr Phyto Lib only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25370 and BPHYT_RS25375 are separated by 350 nucleotidesBPHYT_RS25375 and BPHYT_RS25380 are separated by 13 nucleotides BPHYT_RS25370: BPHYT_RS25370 - acyl-CoA dehydrogenase, at 1,269,277 to 1,270,419 _RS25370 BPHYT_RS25375: BPHYT_RS25375 - lipid-transfer protein, at 1,270,770 to 1,271,951 _RS25375 BPHYT_RS25380: BPHYT_RS25380 - acyl-CoA dehydrogenase, at 1,271,965 to 1,273,164 _RS25380 Position (kb) 1270 1271 1272Strain fitness (log2 ratio) -2 -1 0 1 2at 1270.549 kb on - strandat 1271.127 kb on + strand, within BPHYT_RS25375at 1271.166 kb on - strand, within BPHYT_RS25375at 1271.166 kb on - strand, within BPHYT_RS25375at 1271.342 kb on - strand, within BPHYT_RS25375at 1271.757 kb on + strand, within BPHYT_RS25375at 1271.940 kb on + strandat 1271.940 kb on + strandat 1271.941 kb on - strandat 1272.416 kb on - strand, within BPHYT_RS25380

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib only
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1,270,549 - +0.7
1,271,127 + BPHYT_RS25375 0.30 -0.1
1,271,166 - BPHYT_RS25375 0.34 -0.3
1,271,166 - BPHYT_RS25375 0.34 -0.6
1,271,342 - BPHYT_RS25375 0.48 -0.8
1,271,757 + BPHYT_RS25375 0.84 +2.5
1,271,940 + +0.3
1,271,940 + -0.4
1,271,941 - -2.5
1,272,416 - BPHYT_RS25380 0.38 +0.9

Or see this region's nucleotide sequence