Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS24525

Experiment: 36hr Phyto Lib only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS24520 and BPHYT_RS24525 are separated by 256 nucleotidesBPHYT_RS24525 and BPHYT_RS24530 are separated by 369 nucleotides BPHYT_RS24520: BPHYT_RS24520 - PAS sensor protein, at 1,067,651 to 1,069,129 _RS24520 BPHYT_RS24525: BPHYT_RS24525 - 2-hydroxyacid dehydrogenase, at 1,069,386 to 1,070,318 _RS24525 BPHYT_RS24530: BPHYT_RS24530 - formate dehydrogenase, at 1,070,688 to 1,073,612 _RS24530 Position (kb) 1069 1070 1071Strain fitness (log2 ratio) -1 0 1at 1068.588 kb on + strand, within BPHYT_RS24520at 1068.589 kb on - strand, within BPHYT_RS24520at 1068.609 kb on + strand, within BPHYT_RS24520at 1068.609 kb on + strand, within BPHYT_RS24520at 1068.610 kb on - strand, within BPHYT_RS24520at 1068.610 kb on - strand, within BPHYT_RS24520at 1068.756 kb on + strand, within BPHYT_RS24520at 1068.757 kb on - strand, within BPHYT_RS24520at 1068.806 kb on + strand, within BPHYT_RS24520at 1068.807 kb on - strand, within BPHYT_RS24520at 1069.332 kb on - strandat 1069.541 kb on + strand, within BPHYT_RS24525at 1069.541 kb on + strand, within BPHYT_RS24525at 1069.893 kb on + strand, within BPHYT_RS24525at 1070.270 kb on + strandat 1070.381 kb on + strandat 1070.382 kb on - strandat 1070.434 kb on + strandat 1070.623 kb on - strandat 1070.745 kb on + strandat 1070.746 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib only
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1,068,588 + BPHYT_RS24520 0.63 +0.4
1,068,589 - BPHYT_RS24520 0.63 +1.7
1,068,609 + BPHYT_RS24520 0.65 +0.1
1,068,609 + BPHYT_RS24520 0.65 +0.0
1,068,610 - BPHYT_RS24520 0.65 -0.4
1,068,610 - BPHYT_RS24520 0.65 +0.4
1,068,756 + BPHYT_RS24520 0.75 +0.5
1,068,757 - BPHYT_RS24520 0.75 +0.5
1,068,806 + BPHYT_RS24520 0.78 +1.7
1,068,807 - BPHYT_RS24520 0.78 +0.9
1,069,332 - +0.6
1,069,541 + BPHYT_RS24525 0.17 +1.0
1,069,541 + BPHYT_RS24525 0.17 +0.0
1,069,893 + BPHYT_RS24525 0.54 +1.0
1,070,270 + -1.0
1,070,381 + -0.0
1,070,382 - -0.1
1,070,434 + -0.4
1,070,623 - -0.1
1,070,745 + -0.5
1,070,746 - -0.6

Or see this region's nucleotide sequence