Experiment: 36hr Phyto Lib only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23085 and BPHYT_RS23090 are separated by 33 nucleotides BPHYT_RS23090 and BPHYT_RS23095 are separated by 37 nucleotides BPHYT_RS23095 and BPHYT_RS23100 are separated by 26 nucleotides
BPHYT_RS23085: BPHYT_RS23085 - MFS transporter, at 743,089 to 744,501
_RS23085
BPHYT_RS23090: BPHYT_RS23090 - histidine kinase, at 744,535 to 745,089
_RS23090
BPHYT_RS23095: BPHYT_RS23095 - MFS transporter, at 745,127 to 746,359
_RS23095
BPHYT_RS23100: BPHYT_RS23100 - sugar ABC transporter substrate-binding protein, at 746,386 to 747,333
_RS23100
Position (kb) 745
746
747 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 744.202 kb on + strand, within BPHYT_RS23085 at 744.203 kb on - strand, within BPHYT_RS23085 at 744.209 kb on - strand, within BPHYT_RS23085 at 744.915 kb on - strand, within BPHYT_RS23090 at 745.221 kb on - strand at 745.221 kb on - strand at 745.508 kb on + strand, within BPHYT_RS23095 at 745.634 kb on + strand, within BPHYT_RS23095 at 745.868 kb on + strand, within BPHYT_RS23095 at 745.929 kb on - strand, within BPHYT_RS23095 at 746.186 kb on - strand, within BPHYT_RS23095 at 746.330 kb on + strand at 746.387 kb on + strand at 746.388 kb on - strand at 746.388 kb on - strand at 746.512 kb on + strand, within BPHYT_RS23100 at 746.513 kb on - strand, within BPHYT_RS23100
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib only remove 744,202 + BPHYT_RS23085 0.79 +0.1 744,203 - BPHYT_RS23085 0.79 -2.0 744,209 - BPHYT_RS23085 0.79 -0.4 744,915 - BPHYT_RS23090 0.68 -0.6 745,221 - +2.9 745,221 - +0.9 745,508 + BPHYT_RS23095 0.31 +1.9 745,634 + BPHYT_RS23095 0.41 +0.2 745,868 + BPHYT_RS23095 0.60 +0.1 745,929 - BPHYT_RS23095 0.65 -0.2 746,186 - BPHYT_RS23095 0.86 -0.8 746,330 + +0.2 746,387 + -0.5 746,388 - -0.0 746,388 - -1.0 746,512 + BPHYT_RS23100 0.13 -1.4 746,513 - BPHYT_RS23100 0.13 -2.2
Or see this region's nucleotide sequence