Experiment: 36hr Phyto Lib only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23080 and BPHYT_RS23085 are separated by 48 nucleotides BPHYT_RS23085 and BPHYT_RS23090 are separated by 33 nucleotides BPHYT_RS23090 and BPHYT_RS23095 are separated by 37 nucleotides
BPHYT_RS23080: BPHYT_RS23080 - gamma-glutamyltransferase, at 741,256 to 743,040
_RS23080
BPHYT_RS23085: BPHYT_RS23085 - MFS transporter, at 743,089 to 744,501
_RS23085
BPHYT_RS23090: BPHYT_RS23090 - histidine kinase, at 744,535 to 745,089
_RS23090
BPHYT_RS23095: BPHYT_RS23095 - MFS transporter, at 745,127 to 746,359
_RS23095
Position (kb) 743
744
745 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 742.182 kb on + strand, within BPHYT_RS23080 at 743.231 kb on + strand, within BPHYT_RS23085 at 743.278 kb on + strand, within BPHYT_RS23085 at 743.279 kb on - strand, within BPHYT_RS23085 at 743.368 kb on + strand, within BPHYT_RS23085 at 743.555 kb on - strand, within BPHYT_RS23085 at 743.847 kb on + strand, within BPHYT_RS23085 at 743.924 kb on - strand, within BPHYT_RS23085 at 744.029 kb on - strand, within BPHYT_RS23085 at 744.202 kb on + strand, within BPHYT_RS23085 at 744.203 kb on - strand, within BPHYT_RS23085 at 744.209 kb on - strand, within BPHYT_RS23085 at 744.915 kb on - strand, within BPHYT_RS23090 at 745.221 kb on - strand at 745.221 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib only remove 742,182 + BPHYT_RS23080 0.52 +0.5 743,231 + BPHYT_RS23085 0.10 -0.1 743,278 + BPHYT_RS23085 0.13 -1.2 743,279 - BPHYT_RS23085 0.13 +0.0 743,368 + BPHYT_RS23085 0.20 -1.3 743,555 - BPHYT_RS23085 0.33 +1.4 743,847 + BPHYT_RS23085 0.54 +1.0 743,924 - BPHYT_RS23085 0.59 +0.6 744,029 - BPHYT_RS23085 0.67 -0.2 744,202 + BPHYT_RS23085 0.79 +0.1 744,203 - BPHYT_RS23085 0.79 -2.0 744,209 - BPHYT_RS23085 0.79 -0.4 744,915 - BPHYT_RS23090 0.68 -0.6 745,221 - +2.9 745,221 - +0.9
Or see this region's nucleotide sequence