Experiment: 36hr Phyto Lib only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14330 and BPHYT_RS14335 are separated by 281 nucleotides BPHYT_RS14335 and BPHYT_RS14340 are separated by 325 nucleotides BPHYT_RS14340 and BPHYT_RS14345 are separated by 100 nucleotides
BPHYT_RS14330: BPHYT_RS14330 - elongation factor P, at 3,272,601 to 3,273,158
_RS14330
BPHYT_RS14335: BPHYT_RS14335 - hypothetical protein, at 3,273,440 to 3,273,646
_RS14335
BPHYT_RS14340: BPHYT_RS14340 - Fis family transcriptional regulator, at 3,273,972 to 3,275,354
_RS14340
BPHYT_RS14345: BPHYT_RS14345 - beta-hexosaminidase, at 3,275,455 to 3,276,483
_RS14345
Position (kb)
3273
3274
3275
3276 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3273.262 kb on - strand at 3273.262 kb on - strand at 3273.262 kb on - strand at 3273.350 kb on + strand at 3273.350 kb on + strand at 3273.351 kb on - strand at 3273.351 kb on - strand at 3273.397 kb on - strand at 3273.399 kb on - strand at 3273.579 kb on - strand, within BPHYT_RS14335 at 3273.665 kb on + strand at 3273.666 kb on - strand at 3273.666 kb on - strand at 3273.696 kb on - strand at 3273.696 kb on - strand at 3273.696 kb on - strand at 3273.714 kb on - strand at 3273.799 kb on + strand at 3273.800 kb on - strand at 3273.800 kb on - strand at 3273.800 kb on - strand at 3273.800 kb on - strand at 3273.834 kb on - strand at 3273.834 kb on - strand at 3273.841 kb on - strand at 3273.923 kb on - strand at 3274.008 kb on - strand at 3274.272 kb on + strand, within BPHYT_RS14340 at 3274.335 kb on + strand, within BPHYT_RS14340 at 3274.335 kb on + strand, within BPHYT_RS14340 at 3274.335 kb on + strand, within BPHYT_RS14340 at 3274.384 kb on + strand, within BPHYT_RS14340 at 3274.385 kb on - strand, within BPHYT_RS14340 at 3274.385 kb on - strand, within BPHYT_RS14340 at 3274.434 kb on + strand, within BPHYT_RS14340 at 3274.434 kb on + strand, within BPHYT_RS14340 at 3274.435 kb on - strand, within BPHYT_RS14340 at 3274.452 kb on - strand, within BPHYT_RS14340 at 3274.779 kb on - strand, within BPHYT_RS14340 at 3274.779 kb on - strand, within BPHYT_RS14340 at 3274.779 kb on - strand, within BPHYT_RS14340 at 3274.779 kb on - strand, within BPHYT_RS14340 at 3274.779 kb on - strand, within BPHYT_RS14340 at 3275.061 kb on + strand, within BPHYT_RS14340 at 3275.062 kb on - strand, within BPHYT_RS14340 at 3275.080 kb on + strand, within BPHYT_RS14340 at 3275.080 kb on + strand, within BPHYT_RS14340 at 3275.080 kb on + strand, within BPHYT_RS14340 at 3275.080 kb on + strand, within BPHYT_RS14340 at 3275.081 kb on - strand, within BPHYT_RS14340 at 3275.112 kb on + strand, within BPHYT_RS14340 at 3275.320 kb on + strand at 3275.320 kb on + strand at 3275.320 kb on + strand at 3275.320 kb on + strand at 3275.321 kb on - strand at 3275.322 kb on + strand at 3275.322 kb on + strand at 3275.322 kb on + strand at 3275.339 kb on + strand at 3275.339 kb on + strand at 3275.339 kb on + strand at 3275.340 kb on - strand at 3275.340 kb on - strand at 3275.340 kb on - strand at 3275.353 kb on - strand at 3275.407 kb on + strand at 3275.407 kb on + strand at 3275.456 kb on + strand at 3275.495 kb on + strand at 3275.873 kb on + strand, within BPHYT_RS14345 at 3276.093 kb on - strand, within BPHYT_RS14345 at 3276.161 kb on + strand, within BPHYT_RS14345 at 3276.162 kb on - strand, within BPHYT_RS14345 at 3276.162 kb on - strand, within BPHYT_RS14345 at 3276.162 kb on - strand, within BPHYT_RS14345
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib only remove 3,273,262 - +0.1 3,273,262 - -0.8 3,273,262 - +0.6 3,273,350 + -0.6 3,273,350 + +1.3 3,273,351 - +0.6 3,273,351 - +0.4 3,273,397 - +0.6 3,273,399 - +0.3 3,273,579 - BPHYT_RS14335 0.67 -1.0 3,273,665 + -1.0 3,273,666 - +0.4 3,273,666 - +0.7 3,273,696 - +0.9 3,273,696 - +1.3 3,273,696 - +1.0 3,273,714 - +0.6 3,273,799 + -1.1 3,273,800 - +0.6 3,273,800 - +1.1 3,273,800 - +0.8 3,273,800 - +1.6 3,273,834 - -0.8 3,273,834 - -1.0 3,273,841 - +0.2 3,273,923 - +0.8 3,274,008 - +2.6 3,274,272 + BPHYT_RS14340 0.22 +0.5 3,274,335 + BPHYT_RS14340 0.26 +0.5 3,274,335 + BPHYT_RS14340 0.26 +0.3 3,274,335 + BPHYT_RS14340 0.26 +2.3 3,274,384 + BPHYT_RS14340 0.30 +0.7 3,274,385 - BPHYT_RS14340 0.30 +1.3 3,274,385 - BPHYT_RS14340 0.30 +0.6 3,274,434 + BPHYT_RS14340 0.33 +1.7 3,274,434 + BPHYT_RS14340 0.33 +1.4 3,274,435 - BPHYT_RS14340 0.33 +0.4 3,274,452 - BPHYT_RS14340 0.35 +0.3 3,274,779 - BPHYT_RS14340 0.58 +0.3 3,274,779 - BPHYT_RS14340 0.58 +3.7 3,274,779 - BPHYT_RS14340 0.58 +1.1 3,274,779 - BPHYT_RS14340 0.58 +1.3 3,274,779 - BPHYT_RS14340 0.58 +0.9 3,275,061 + BPHYT_RS14340 0.79 +0.7 3,275,062 - BPHYT_RS14340 0.79 +0.2 3,275,080 + BPHYT_RS14340 0.80 +0.7 3,275,080 + BPHYT_RS14340 0.80 +0.9 3,275,080 + BPHYT_RS14340 0.80 -0.1 3,275,080 + BPHYT_RS14340 0.80 +0.4 3,275,081 - BPHYT_RS14340 0.80 -0.1 3,275,112 + BPHYT_RS14340 0.82 +0.1 3,275,320 + +1.9 3,275,320 + +0.7 3,275,320 + +0.8 3,275,320 + +0.8 3,275,321 - +0.9 3,275,322 + +1.7 3,275,322 + +0.6 3,275,322 + +0.8 3,275,339 + -0.1 3,275,339 + +0.8 3,275,339 + +1.0 3,275,340 - +0.9 3,275,340 - +0.7 3,275,340 - +0.6 3,275,353 - -1.5 3,275,407 + -0.3 3,275,407 + -0.7 3,275,456 + +0.2 3,275,495 + -0.5 3,275,873 + BPHYT_RS14345 0.41 +1.6 3,276,093 - BPHYT_RS14345 0.62 +1.0 3,276,161 + BPHYT_RS14345 0.69 -1.0 3,276,162 - BPHYT_RS14345 0.69 +0.8 3,276,162 - BPHYT_RS14345 0.69 -2.1 3,276,162 - BPHYT_RS14345 0.69 +1.6
Or see this region's nucleotide sequence