Experiment: 36hr Phyto Lib only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05105 and BPHYT_RS05110 are separated by 206 nucleotides BPHYT_RS05110 and BPHYT_RS05115 are separated by 220 nucleotides BPHYT_RS05115 and BPHYT_RS05120 overlap by 4 nucleotides BPHYT_RS05120 and BPHYT_RS05125 are separated by 12 nucleotides
BPHYT_RS05105: BPHYT_RS05105 - serine--tRNA ligase, at 1,178,342 to 1,179,637
_RS05105
BPHYT_RS05110: BPHYT_RS05110 - tRNA-Ser, at 1,179,844 to 1,179,934
_RS05110
BPHYT_RS05115: BPHYT_RS05115 - NADH-ubiquinone oxidoreductase subunit 6, at 1,180,155 to 1,181,456
_RS05115
BPHYT_RS05120: BPHYT_RS05120 - hypothetical protein, at 1,181,453 to 1,182,304
_RS05120
BPHYT_RS05125: BPHYT_RS05125 - cyclopropane-fatty-acyl-phospholipid synthase, at 1,182,317 to 1,183,528
_RS05125
Position (kb) 1180
1181
1182 Strain fitness (log2 ratio) -1
0
1 at 1179.662 kb on + strand at 1179.663 kb on - strand at 1180.078 kb on + strand at 1180.079 kb on - strand at 1180.079 kb on - strand at 1180.311 kb on - strand, within BPHYT_RS05115 at 1180.403 kb on - strand, within BPHYT_RS05115 at 1180.831 kb on - strand, within BPHYT_RS05115 at 1180.831 kb on - strand, within BPHYT_RS05115 at 1180.841 kb on + strand, within BPHYT_RS05115 at 1180.841 kb on + strand, within BPHYT_RS05115 at 1180.841 kb on + strand, within BPHYT_RS05115 at 1180.842 kb on - strand, within BPHYT_RS05115 at 1180.843 kb on + strand, within BPHYT_RS05115 at 1180.844 kb on - strand, within BPHYT_RS05115 at 1181.040 kb on + strand, within BPHYT_RS05115 at 1181.119 kb on - strand, within BPHYT_RS05115 at 1181.166 kb on + strand, within BPHYT_RS05115 at 1181.182 kb on + strand, within BPHYT_RS05115 at 1181.452 kb on + strand at 1181.555 kb on + strand, within BPHYT_RS05120 at 1181.556 kb on - strand, within BPHYT_RS05120 at 1181.568 kb on - strand, within BPHYT_RS05120 at 1181.710 kb on - strand, within BPHYT_RS05120 at 1181.710 kb on - strand, within BPHYT_RS05120 at 1181.880 kb on - strand, within BPHYT_RS05120 at 1181.880 kb on - strand, within BPHYT_RS05120 at 1181.880 kb on - strand, within BPHYT_RS05120 at 1181.982 kb on - strand, within BPHYT_RS05120 at 1182.032 kb on + strand, within BPHYT_RS05120
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib only remove 1,179,662 + +0.1 1,179,663 - -1.0 1,180,078 + +1.6 1,180,079 - +0.7 1,180,079 - +0.2 1,180,311 - BPHYT_RS05115 0.12 -1.5 1,180,403 - BPHYT_RS05115 0.19 -1.5 1,180,831 - BPHYT_RS05115 0.52 +1.6 1,180,831 - BPHYT_RS05115 0.52 +0.4 1,180,841 + BPHYT_RS05115 0.53 -1.4 1,180,841 + BPHYT_RS05115 0.53 -0.6 1,180,841 + BPHYT_RS05115 0.53 -0.4 1,180,842 - BPHYT_RS05115 0.53 -0.4 1,180,843 + BPHYT_RS05115 0.53 -0.2 1,180,844 - BPHYT_RS05115 0.53 -0.8 1,181,040 + BPHYT_RS05115 0.68 +0.1 1,181,119 - BPHYT_RS05115 0.74 -1.0 1,181,166 + BPHYT_RS05115 0.78 -0.5 1,181,182 + BPHYT_RS05115 0.79 -0.2 1,181,452 + -1.0 1,181,555 + BPHYT_RS05120 0.12 +0.1 1,181,556 - BPHYT_RS05120 0.12 +0.5 1,181,568 - BPHYT_RS05120 0.13 +0.3 1,181,710 - BPHYT_RS05120 0.30 -0.4 1,181,710 - BPHYT_RS05120 0.30 -0.6 1,181,880 - BPHYT_RS05120 0.50 -1.1 1,181,880 - BPHYT_RS05120 0.50 -0.4 1,181,880 - BPHYT_RS05120 0.50 -0.3 1,181,982 - BPHYT_RS05120 0.62 -0.0 1,182,032 + BPHYT_RS05120 0.68 -0.1
Or see this region's nucleotide sequence