Experiment: 36hr Phyto Lib only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00500 and BPHYT_RS00505 are separated by 184 nucleotides BPHYT_RS00505 and BPHYT_RS00510 are separated by 51 nucleotides BPHYT_RS00510 and BPHYT_RS00515 are separated by 54 nucleotides BPHYT_RS00515 and BPHYT_RS00520 are separated by 193 nucleotides
BPHYT_RS00500: BPHYT_RS00500 - hypothetical protein, at 121,647 to 122,987
_RS00500
BPHYT_RS00505: BPHYT_RS00505 - glyoxalase, at 123,172 to 123,570
_RS00505
BPHYT_RS00510: BPHYT_RS00510 - S-(hydroxymethyl)glutathione dehydrogenase, at 123,622 to 124,731
_RS00510
BPHYT_RS00515: BPHYT_RS00515 - regulator, at 124,786 to 125,061
_RS00515
BPHYT_RS00520: BPHYT_RS00520 - glutamate--cysteine ligase, at 125,255 to 126,853
_RS00520
Position (kb) 123
124
125 Strain fitness (log2 ratio) -2
-1
0
1
2 at 122.668 kb on + strand, within BPHYT_RS00500 at 122.668 kb on + strand, within BPHYT_RS00500 at 122.669 kb on - strand, within BPHYT_RS00500 at 122.669 kb on - strand, within BPHYT_RS00500 at 122.839 kb on - strand, within BPHYT_RS00500 at 122.912 kb on - strand at 122.963 kb on + strand at 122.964 kb on - strand at 122.964 kb on - strand at 122.964 kb on - strand at 123.020 kb on + strand at 123.057 kb on + strand at 123.058 kb on - strand at 123.125 kb on + strand at 123.125 kb on + strand at 123.185 kb on + strand at 123.221 kb on + strand, within BPHYT_RS00505 at 123.231 kb on - strand, within BPHYT_RS00505 at 123.419 kb on + strand, within BPHYT_RS00505 at 123.420 kb on - strand, within BPHYT_RS00505 at 123.605 kb on - strand at 123.605 kb on - strand at 124.010 kb on + strand, within BPHYT_RS00510 at 124.047 kb on + strand, within BPHYT_RS00510 at 124.047 kb on + strand, within BPHYT_RS00510 at 124.295 kb on - strand, within BPHYT_RS00510 at 124.328 kb on + strand, within BPHYT_RS00510 at 124.329 kb on - strand, within BPHYT_RS00510 at 124.352 kb on + strand, within BPHYT_RS00510 at 124.369 kb on - strand, within BPHYT_RS00510 at 124.608 kb on + strand, within BPHYT_RS00510 at 124.747 kb on - strand at 125.054 kb on + strand at 125.082 kb on - strand at 125.095 kb on + strand at 125.218 kb on - strand at 125.256 kb on + strand at 125.256 kb on + strand at 125.398 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib only remove 122,668 + BPHYT_RS00500 0.76 -0.1 122,668 + BPHYT_RS00500 0.76 -0.8 122,669 - BPHYT_RS00500 0.76 +0.4 122,669 - BPHYT_RS00500 0.76 +1.1 122,839 - BPHYT_RS00500 0.89 -1.4 122,912 - -0.1 122,963 + +0.8 122,964 - -0.3 122,964 - -0.1 122,964 - -0.2 123,020 + +0.3 123,057 + -0.9 123,058 - -0.9 123,125 + -0.5 123,125 + -0.2 123,185 + -1.1 123,221 + BPHYT_RS00505 0.12 -0.0 123,231 - BPHYT_RS00505 0.15 -2.1 123,419 + BPHYT_RS00505 0.62 +0.8 123,420 - BPHYT_RS00505 0.62 -0.9 123,605 - -0.8 123,605 - +1.0 124,010 + BPHYT_RS00510 0.35 -1.2 124,047 + BPHYT_RS00510 0.38 -0.5 124,047 + BPHYT_RS00510 0.38 -0.2 124,295 - BPHYT_RS00510 0.61 -1.1 124,328 + BPHYT_RS00510 0.64 -1.2 124,329 - BPHYT_RS00510 0.64 -1.7 124,352 + BPHYT_RS00510 0.66 -0.2 124,369 - BPHYT_RS00510 0.67 -0.2 124,608 + BPHYT_RS00510 0.89 -0.6 124,747 - -1.3 125,054 + +2.6 125,082 - +0.2 125,095 + -0.5 125,218 - -0.5 125,256 + -0.8 125,256 + -0.2 125,398 + +2.5
Or see this region's nucleotide sequence