Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25035

Experiment: 36hr PHYTO11 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25030 and BPHYT_RS25035 are separated by 86 nucleotidesBPHYT_RS25035 and BPHYT_RS25040 are separated by 197 nucleotidesBPHYT_RS25040 and BPHYT_RS25045 are separated by 133 nucleotides BPHYT_RS25030: BPHYT_RS25030 - gluconate 2-dehydrogenase, at 1,185,696 to 1,186,469 _RS25030 BPHYT_RS25035: BPHYT_RS25035 - long-chain fatty acid--CoA ligase, at 1,186,556 to 1,188,253 _RS25035 BPHYT_RS25040: BPHYT_RS25040 - hypothetical protein, at 1,188,451 to 1,188,741 _RS25040 BPHYT_RS25045: BPHYT_RS25045 - LysR family transcriptional regulator, at 1,188,875 to 1,189,786 _RS25045 Position (kb) 1186 1187 1188 1189Strain fitness (log2 ratio) -2 -1 0 1at 1185.564 kb on - strandat 1185.633 kb on - strandat 1185.633 kb on - strandat 1185.965 kb on + strand, within BPHYT_RS25030at 1186.186 kb on - strand, within BPHYT_RS25030at 1186.344 kb on - strand, within BPHYT_RS25030at 1186.486 kb on - strandat 1186.631 kb on + strandat 1186.792 kb on + strand, within BPHYT_RS25035at 1186.823 kb on + strand, within BPHYT_RS25035at 1187.015 kb on + strand, within BPHYT_RS25035at 1187.015 kb on + strand, within BPHYT_RS25035at 1187.413 kb on - strand, within BPHYT_RS25035at 1187.499 kb on - strand, within BPHYT_RS25035at 1187.592 kb on - strand, within BPHYT_RS25035at 1188.330 kb on + strandat 1188.331 kb on - strandat 1188.960 kb on + strandat 1189.024 kb on - strand, within BPHYT_RS25045at 1189.024 kb on - strand, within BPHYT_RS25045

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 0.1
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1,185,564 - +0.7
1,185,633 - +1.4
1,185,633 - +0.3
1,185,965 + BPHYT_RS25030 0.35 -0.2
1,186,186 - BPHYT_RS25030 0.63 +1.2
1,186,344 - BPHYT_RS25030 0.84 -0.3
1,186,486 - -0.3
1,186,631 + +0.7
1,186,792 + BPHYT_RS25035 0.14 -1.2
1,186,823 + BPHYT_RS25035 0.16 -1.0
1,187,015 + BPHYT_RS25035 0.27 -1.1
1,187,015 + BPHYT_RS25035 0.27 -2.3
1,187,413 - BPHYT_RS25035 0.50 +0.5
1,187,499 - BPHYT_RS25035 0.56 -2.4
1,187,592 - BPHYT_RS25035 0.61 -0.5
1,188,330 + -2.2
1,188,331 - +0.2
1,188,960 + -1.2
1,189,024 - BPHYT_RS25045 0.16 +0.1
1,189,024 - BPHYT_RS25045 0.16 -0.2

Or see this region's nucleotide sequence