Experiment: 36hr PHYTO11 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22680 and BPHYT_RS22685 overlap by 4 nucleotides BPHYT_RS22685 and BPHYT_RS22690 are separated by 49 nucleotides BPHYT_RS22690 and BPHYT_RS22695 are separated by 70 nucleotides BPHYT_RS22695 and BPHYT_RS22700 are separated by 94 nucleotides
BPHYT_RS22680: BPHYT_RS22680 - 3-dehydroquinate dehydratase, at 644,503 to 644,958
_RS22680
BPHYT_RS22685: BPHYT_RS22685 - shikimate 5-dehydrogenase, at 644,955 to 645,869
_RS22685
BPHYT_RS22690: BPHYT_RS22690 - D-galactonate transporter, at 645,919 to 647,295
_RS22690
BPHYT_RS22695: BPHYT_RS22695 - TetR family transcriptional regulator, at 647,366 to 648,061
_RS22695
BPHYT_RS22700: BPHYT_RS22700 - FAD-dependent oxidoreductase, at 648,156 to 649,436
_RS22700
Position (kb) 645
646
647
648 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 644.938 kb on - strand at 645.225 kb on + strand, within BPHYT_RS22685 at 645.225 kb on + strand, within BPHYT_RS22685 at 645.696 kb on + strand, within BPHYT_RS22685 at 646.007 kb on - strand at 646.048 kb on + strand at 646.153 kb on + strand, within BPHYT_RS22690 at 646.153 kb on + strand, within BPHYT_RS22690 at 646.153 kb on + strand, within BPHYT_RS22690 at 646.153 kb on + strand, within BPHYT_RS22690 at 646.153 kb on + strand, within BPHYT_RS22690 at 646.154 kb on - strand, within BPHYT_RS22690 at 646.154 kb on - strand, within BPHYT_RS22690 at 646.214 kb on - strand, within BPHYT_RS22690 at 646.396 kb on + strand, within BPHYT_RS22690 at 646.483 kb on + strand, within BPHYT_RS22690 at 646.534 kb on + strand, within BPHYT_RS22690 at 646.535 kb on - strand, within BPHYT_RS22690 at 646.582 kb on + strand, within BPHYT_RS22690 at 646.583 kb on - strand, within BPHYT_RS22690 at 646.585 kb on - strand, within BPHYT_RS22690 at 646.786 kb on + strand, within BPHYT_RS22690 at 646.786 kb on + strand, within BPHYT_RS22690 at 646.806 kb on + strand, within BPHYT_RS22690 at 646.987 kb on + strand, within BPHYT_RS22690 at 646.987 kb on + strand, within BPHYT_RS22690 at 646.987 kb on + strand, within BPHYT_RS22690 at 646.987 kb on + strand, within BPHYT_RS22690 at 646.987 kb on + strand, within BPHYT_RS22690 at 646.988 kb on - strand, within BPHYT_RS22690 at 646.988 kb on - strand, within BPHYT_RS22690 at 647.473 kb on + strand, within BPHYT_RS22695 at 647.474 kb on - strand, within BPHYT_RS22695 at 647.594 kb on - strand, within BPHYT_RS22695 at 647.644 kb on - strand, within BPHYT_RS22695 at 647.717 kb on - strand, within BPHYT_RS22695 at 647.781 kb on - strand, within BPHYT_RS22695 at 647.799 kb on + strand, within BPHYT_RS22695 at 647.800 kb on - strand, within BPHYT_RS22695 at 647.800 kb on - strand, within BPHYT_RS22695 at 647.800 kb on - strand, within BPHYT_RS22695 at 647.800 kb on - strand, within BPHYT_RS22695 at 647.847 kb on + strand, within BPHYT_RS22695 at 647.848 kb on - strand, within BPHYT_RS22695 at 647.853 kb on + strand, within BPHYT_RS22695 at 647.853 kb on + strand, within BPHYT_RS22695 at 647.854 kb on - strand, within BPHYT_RS22695 at 647.980 kb on - strand, within BPHYT_RS22695 at 647.980 kb on - strand, within BPHYT_RS22695 at 648.094 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 0.1 remove 644,938 - -2.0 645,225 + BPHYT_RS22685 0.30 +0.2 645,225 + BPHYT_RS22685 0.30 -0.2 645,696 + BPHYT_RS22685 0.81 -1.2 646,007 - -0.1 646,048 + -0.8 646,153 + BPHYT_RS22690 0.17 +0.0 646,153 + BPHYT_RS22690 0.17 -1.0 646,153 + BPHYT_RS22690 0.17 +0.2 646,153 + BPHYT_RS22690 0.17 +1.7 646,153 + BPHYT_RS22690 0.17 -0.4 646,154 - BPHYT_RS22690 0.17 +2.5 646,154 - BPHYT_RS22690 0.17 +0.1 646,214 - BPHYT_RS22690 0.21 -1.2 646,396 + BPHYT_RS22690 0.35 -0.2 646,483 + BPHYT_RS22690 0.41 +1.5 646,534 + BPHYT_RS22690 0.45 +0.6 646,535 - BPHYT_RS22690 0.45 -0.3 646,582 + BPHYT_RS22690 0.48 -3.2 646,583 - BPHYT_RS22690 0.48 +0.1 646,585 - BPHYT_RS22690 0.48 -1.5 646,786 + BPHYT_RS22690 0.63 +0.2 646,786 + BPHYT_RS22690 0.63 +0.1 646,806 + BPHYT_RS22690 0.64 +0.2 646,987 + BPHYT_RS22690 0.78 +0.3 646,987 + BPHYT_RS22690 0.78 -0.5 646,987 + BPHYT_RS22690 0.78 +0.1 646,987 + BPHYT_RS22690 0.78 +2.5 646,987 + BPHYT_RS22690 0.78 -0.5 646,988 - BPHYT_RS22690 0.78 -0.3 646,988 - BPHYT_RS22690 0.78 -0.6 647,473 + BPHYT_RS22695 0.15 -0.7 647,474 - BPHYT_RS22695 0.16 +0.7 647,594 - BPHYT_RS22695 0.33 -0.2 647,644 - BPHYT_RS22695 0.40 -0.1 647,717 - BPHYT_RS22695 0.50 -0.2 647,781 - BPHYT_RS22695 0.60 -0.2 647,799 + BPHYT_RS22695 0.62 -0.8 647,800 - BPHYT_RS22695 0.62 -0.4 647,800 - BPHYT_RS22695 0.62 -0.5 647,800 - BPHYT_RS22695 0.62 +1.3 647,800 - BPHYT_RS22695 0.62 -0.1 647,847 + BPHYT_RS22695 0.69 +0.4 647,848 - BPHYT_RS22695 0.69 +0.9 647,853 + BPHYT_RS22695 0.70 -1.6 647,853 + BPHYT_RS22695 0.70 -0.0 647,854 - BPHYT_RS22695 0.70 +1.7 647,980 - BPHYT_RS22695 0.88 -1.0 647,980 - BPHYT_RS22695 0.88 -1.4 648,094 - +0.1
Or see this region's nucleotide sequence