Experiment: 36hr PHYTO11 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16130 and BPHYT_RS16135 overlap by 4 nucleotides BPHYT_RS16135 and BPHYT_RS16140 overlap by 8 nucleotides BPHYT_RS16140 and BPHYT_RS16145 are separated by 58 nucleotides BPHYT_RS16145 and BPHYT_RS16150 are separated by 14 nucleotides
BPHYT_RS16130: BPHYT_RS16130 - cation ABC transporter substrate-binding protein, at 3,658,958 to 3,659,926
_RS16130
BPHYT_RS16135: BPHYT_RS16135 - ABC transporter ATP-binding protein, at 3,659,923 to 3,660,864
_RS16135
BPHYT_RS16140: BPHYT_RS16140 - ABC transporter permease, at 3,660,857 to 3,661,639
_RS16140
BPHYT_RS16145: BPHYT_RS16145 - S-formylglutathione hydrolase, at 3,661,698 to 3,662,549
_RS16145
BPHYT_RS16150: BPHYT_RS16150 - alcohol dehydrogenase, at 3,662,564 to 3,663,670
_RS16150
Position (kb)
3660
3661
3662 Strain fitness (log2 ratio)
-2
-1
0
1 at 3660.194 kb on - strand, within BPHYT_RS16135 at 3660.195 kb on + strand, within BPHYT_RS16135 at 3660.196 kb on - strand, within BPHYT_RS16135 at 3660.377 kb on + strand, within BPHYT_RS16135 at 3660.378 kb on - strand, within BPHYT_RS16135 at 3660.593 kb on - strand, within BPHYT_RS16135 at 3660.593 kb on - strand, within BPHYT_RS16135 at 3660.593 kb on - strand, within BPHYT_RS16135 at 3660.635 kb on + strand, within BPHYT_RS16135 at 3660.636 kb on - strand, within BPHYT_RS16135 at 3661.229 kb on - strand, within BPHYT_RS16140 at 3661.559 kb on + strand, within BPHYT_RS16140 at 3661.674 kb on - strand at 3661.759 kb on + strand at 3661.759 kb on + strand at 3661.760 kb on - strand at 3661.760 kb on - strand at 3661.763 kb on - strand at 3661.774 kb on + strand at 3661.942 kb on + strand, within BPHYT_RS16145 at 3661.942 kb on + strand, within BPHYT_RS16145 at 3662.179 kb on + strand, within BPHYT_RS16145 at 3662.180 kb on - strand, within BPHYT_RS16145 at 3662.180 kb on - strand, within BPHYT_RS16145 at 3662.180 kb on - strand, within BPHYT_RS16145 at 3662.180 kb on - strand, within BPHYT_RS16145 at 3662.185 kb on + strand, within BPHYT_RS16145 at 3662.185 kb on + strand, within BPHYT_RS16145 at 3662.185 kb on + strand, within BPHYT_RS16145 at 3662.185 kb on + strand, within BPHYT_RS16145 at 3662.398 kb on + strand, within BPHYT_RS16145 at 3662.398 kb on + strand, within BPHYT_RS16145 at 3662.399 kb on - strand, within BPHYT_RS16145 at 3662.399 kb on - strand, within BPHYT_RS16145 at 3662.450 kb on - strand, within BPHYT_RS16145 at 3662.450 kb on - strand, within BPHYT_RS16145 at 3662.450 kb on - strand, within BPHYT_RS16145 at 3662.497 kb on + strand at 3662.498 kb on - strand at 3662.568 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 0.1 remove 3,660,194 - BPHYT_RS16135 0.29 +0.4 3,660,195 + BPHYT_RS16135 0.29 +0.1 3,660,196 - BPHYT_RS16135 0.29 +0.2 3,660,377 + BPHYT_RS16135 0.48 -1.2 3,660,378 - BPHYT_RS16135 0.48 +0.5 3,660,593 - BPHYT_RS16135 0.71 -0.7 3,660,593 - BPHYT_RS16135 0.71 -1.3 3,660,593 - BPHYT_RS16135 0.71 -1.2 3,660,635 + BPHYT_RS16135 0.76 +0.1 3,660,636 - BPHYT_RS16135 0.76 +0.3 3,661,229 - BPHYT_RS16140 0.48 +0.2 3,661,559 + BPHYT_RS16140 0.90 +0.2 3,661,674 - -0.6 3,661,759 + -0.5 3,661,759 + -0.5 3,661,760 - -1.9 3,661,760 - -0.9 3,661,763 - -1.0 3,661,774 + +0.7 3,661,942 + BPHYT_RS16145 0.29 -0.3 3,661,942 + BPHYT_RS16145 0.29 -0.1 3,662,179 + BPHYT_RS16145 0.56 -0.6 3,662,180 - BPHYT_RS16145 0.57 +0.1 3,662,180 - BPHYT_RS16145 0.57 -0.5 3,662,180 - BPHYT_RS16145 0.57 +0.8 3,662,180 - BPHYT_RS16145 0.57 -0.3 3,662,185 + BPHYT_RS16145 0.57 +0.6 3,662,185 + BPHYT_RS16145 0.57 -0.5 3,662,185 + BPHYT_RS16145 0.57 +0.3 3,662,185 + BPHYT_RS16145 0.57 -0.1 3,662,398 + BPHYT_RS16145 0.82 +0.2 3,662,398 + BPHYT_RS16145 0.82 +0.1 3,662,399 - BPHYT_RS16145 0.82 -0.7 3,662,399 - BPHYT_RS16145 0.82 -0.3 3,662,450 - BPHYT_RS16145 0.88 -0.2 3,662,450 - BPHYT_RS16145 0.88 +0.1 3,662,450 - BPHYT_RS16145 0.88 +0.5 3,662,497 + -1.6 3,662,498 - -1.5 3,662,568 + +1.3
Or see this region's nucleotide sequence