Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11680

Experiment: 36hr PHYTO11 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11670 and BPHYT_RS11675 are separated by 561 nucleotidesBPHYT_RS11675 and BPHYT_RS11680 are separated by 74 nucleotides BPHYT_RS11670: BPHYT_RS11670 - hypothetical protein, at 2,647,851 to 2,648,114 _RS11670 BPHYT_RS11675: BPHYT_RS11675 - hypothetical protein, at 2,648,676 to 2,649,020 _RS11675 BPHYT_RS11680: BPHYT_RS11680 - protein-L-isoaspartate O-methyltransferase, at 2,649,095 to 2,649,757 _RS11680 Position (kb) 2649 2650Strain fitness (log2 ratio) -2 -1 0 1at 2648.328 kb on + strandat 2648.329 kb on - strandat 2648.332 kb on + strandat 2648.332 kb on + strandat 2648.333 kb on - strandat 2648.333 kb on - strandat 2648.333 kb on - strandat 2648.501 kb on + strandat 2648.502 kb on - strandat 2648.590 kb on - strandat 2649.018 kb on + strandat 2649.171 kb on + strand, within BPHYT_RS11680at 2649.854 kb on - strandat 2649.944 kb on + strandat 2649.944 kb on + strandat 2650.171 kb on - strandat 2650.727 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 0.1
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2,648,328 + -0.1
2,648,329 - -2.1
2,648,332 + +1.4
2,648,332 + -1.2
2,648,333 - -0.6
2,648,333 - -0.3
2,648,333 - -1.1
2,648,501 + -1.2
2,648,502 - -0.6
2,648,590 - -0.5
2,649,018 + -0.7
2,649,171 + BPHYT_RS11680 0.11 +0.9
2,649,854 - -1.5
2,649,944 + +0.1
2,649,944 + +1.3
2,650,171 - -0.1
2,650,727 - -0.7

Or see this region's nucleotide sequence