Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10195

Experiment: 36hr PHYTO11 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10190 and BPHYT_RS10195 are separated by 76 nucleotidesBPHYT_RS10195 and BPHYT_RS10200 are separated by 490 nucleotidesBPHYT_RS10200 and BPHYT_RS10205 are separated by 43 nucleotides BPHYT_RS10190: BPHYT_RS10190 - phosphonate monoester hydrolase, at 2,319,705 to 2,321,276 _RS10190 BPHYT_RS10195: BPHYT_RS10195 - major facilitator transporter, at 2,321,353 to 2,322,690 _RS10195 BPHYT_RS10200: BPHYT_RS10200 - short-chain dehydrogenase, at 2,323,181 to 2,323,561 _RS10200 BPHYT_RS10205: BPHYT_RS10205 - D-lactate dehydrogenase, at 2,323,605 to 2,325,344 _RS10205 Position (kb) 2321 2322 2323Strain fitness (log2 ratio) -3 -2 -1 0 1at 2320.377 kb on + strand, within BPHYT_RS10190at 2320.398 kb on + strand, within BPHYT_RS10190at 2320.398 kb on + strand, within BPHYT_RS10190at 2320.428 kb on + strand, within BPHYT_RS10190at 2320.755 kb on + strand, within BPHYT_RS10190at 2320.941 kb on + strand, within BPHYT_RS10190at 2320.942 kb on - strand, within BPHYT_RS10190at 2321.237 kb on + strandat 2321.437 kb on + strandat 2321.438 kb on - strandat 2321.467 kb on - strandat 2321.467 kb on - strandat 2322.023 kb on + strand, within BPHYT_RS10195at 2322.023 kb on + strand, within BPHYT_RS10195at 2322.412 kb on + strand, within BPHYT_RS10195at 2322.451 kb on + strand, within BPHYT_RS10195at 2322.568 kb on + strandat 2322.597 kb on - strandat 2322.617 kb on + strandat 2322.857 kb on - strandat 2322.930 kb on - strandat 2322.954 kb on - strandat 2323.118 kb on + strandat 2323.119 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 0.1
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2,320,377 + BPHYT_RS10190 0.43 -0.3
2,320,398 + BPHYT_RS10190 0.44 -3.5
2,320,398 + BPHYT_RS10190 0.44 +0.3
2,320,428 + BPHYT_RS10190 0.46 -1.1
2,320,755 + BPHYT_RS10190 0.67 -0.2
2,320,941 + BPHYT_RS10190 0.79 +0.4
2,320,942 - BPHYT_RS10190 0.79 -0.9
2,321,237 + +1.4
2,321,437 + +1.5
2,321,438 - -2.9
2,321,467 - -0.3
2,321,467 - -2.4
2,322,023 + BPHYT_RS10195 0.50 -0.6
2,322,023 + BPHYT_RS10195 0.50 -0.8
2,322,412 + BPHYT_RS10195 0.79 -0.0
2,322,451 + BPHYT_RS10195 0.82 +0.1
2,322,568 + -1.0
2,322,597 - -0.2
2,322,617 + -1.1
2,322,857 - +0.1
2,322,930 - +0.6
2,322,954 - -1.2
2,323,118 + -0.4
2,323,119 - +0.3

Or see this region's nucleotide sequence