Experiment: 36hr PHYTO11 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09215 and BPHYT_RS09220 are separated by 40 nucleotides BPHYT_RS09220 and BPHYT_RS09225 are separated by 39 nucleotides BPHYT_RS09225 and BPHYT_RS09230 are separated by 177 nucleotides
BPHYT_RS09215: BPHYT_RS09215 - ATP-dependent DNA ligase, at 2,085,683 to 2,088,505
_RS09215
BPHYT_RS09220: BPHYT_RS09220 - hypothetical protein, at 2,088,546 to 2,088,851
_RS09220
BPHYT_RS09225: BPHYT_RS09225 - LysR family transcriptional regulator, at 2,088,891 to 2,089,832
_RS09225
BPHYT_RS09230: BPHYT_RS09230 - NADPH:quinone reductase, at 2,090,010 to 2,091,032
_RS09230
Position (kb)
2088
2089
2090 Strain fitness (log2 ratio)
-2
-1
0
1 at 2088.009 kb on + strand, within BPHYT_RS09215 at 2088.010 kb on - strand, within BPHYT_RS09215 at 2088.184 kb on + strand, within BPHYT_RS09215 at 2088.184 kb on + strand, within BPHYT_RS09215 at 2088.184 kb on + strand, within BPHYT_RS09215 at 2088.185 kb on - strand, within BPHYT_RS09215 at 2088.207 kb on - strand, within BPHYT_RS09215 at 2088.504 kb on - strand at 2088.541 kb on - strand at 2088.914 kb on - strand at 2088.914 kb on - strand at 2088.992 kb on - strand, within BPHYT_RS09225 at 2089.028 kb on - strand, within BPHYT_RS09225 at 2089.066 kb on + strand, within BPHYT_RS09225 at 2089.066 kb on + strand, within BPHYT_RS09225 at 2089.067 kb on - strand, within BPHYT_RS09225 at 2089.516 kb on - strand at 2089.741 kb on - strand at 2089.911 kb on + strand at 2089.911 kb on + strand at 2090.010 kb on - strand at 2090.010 kb on - strand at 2090.034 kb on + strand at 2090.035 kb on - strand at 2090.216 kb on + strand, within BPHYT_RS09230 at 2090.275 kb on - strand, within BPHYT_RS09230 at 2090.275 kb on - strand, within BPHYT_RS09230
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 0.1 remove 2,088,009 + BPHYT_RS09215 0.82 -0.8 2,088,010 - BPHYT_RS09215 0.82 -2.2 2,088,184 + BPHYT_RS09215 0.89 -0.3 2,088,184 + BPHYT_RS09215 0.89 +1.2 2,088,184 + BPHYT_RS09215 0.89 -1.6 2,088,185 - BPHYT_RS09215 0.89 -0.4 2,088,207 - BPHYT_RS09215 0.89 -0.4 2,088,504 - -1.2 2,088,541 - +0.6 2,088,914 - -1.1 2,088,914 - +0.0 2,088,992 - BPHYT_RS09225 0.11 -1.2 2,089,028 - BPHYT_RS09225 0.15 -0.2 2,089,066 + BPHYT_RS09225 0.19 +1.3 2,089,066 + BPHYT_RS09225 0.19 -0.5 2,089,067 - BPHYT_RS09225 0.19 -1.0 2,089,516 - +1.3 2,089,741 - +0.4 2,089,911 + -0.6 2,089,911 + -0.3 2,090,010 - -0.3 2,090,010 - -0.8 2,090,034 + +0.3 2,090,035 - -2.7 2,090,216 + BPHYT_RS09230 0.20 -1.3 2,090,275 - BPHYT_RS09230 0.26 -0.4 2,090,275 - BPHYT_RS09230 0.26 -1.0
Or see this region's nucleotide sequence