Experiment: 36hr PHYTO11 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05930 and BPHYT_RS05935 are separated by 80 nucleotides BPHYT_RS05935 and BPHYT_RS05940 are separated by 273 nucleotides BPHYT_RS05940 and BPHYT_RS05945 are separated by 103 nucleotides
BPHYT_RS05930: BPHYT_RS05930 - DNA topoisomerase IV subunit B, at 1,345,964 to 1,347,949
_RS05930
BPHYT_RS05935: BPHYT_RS05935 - DNA topoisomerase IV subunit A, at 1,348,030 to 1,350,354
_RS05935
BPHYT_RS05940: BPHYT_RS05940 - membrane protein, at 1,350,628 to 1,351,086
_RS05940
BPHYT_RS05945: BPHYT_RS05945 - chromate transporter, at 1,351,190 to 1,352,398
_RS05945
Position (kb) 1348
1349
1350
1351 Strain fitness (log2 ratio) -1
0
1
2 at 1350.389 kb on + strand at 1350.390 kb on - strand at 1350.395 kb on - strand at 1350.472 kb on + strand at 1350.472 kb on + strand at 1350.543 kb on + strand at 1350.544 kb on - strand at 1350.749 kb on + strand, within BPHYT_RS05940 at 1350.750 kb on - strand, within BPHYT_RS05940 at 1350.927 kb on + strand, within BPHYT_RS05940 at 1351.254 kb on + strand at 1351.254 kb on + strand at 1351.311 kb on + strand, within BPHYT_RS05945 at 1351.311 kb on + strand, within BPHYT_RS05945
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 0.1 remove 1,350,389 + -0.6 1,350,390 - -1.4 1,350,395 - -1.3 1,350,472 + -0.7 1,350,472 + -0.9 1,350,543 + +0.6 1,350,544 - -0.5 1,350,749 + BPHYT_RS05940 0.26 +0.1 1,350,750 - BPHYT_RS05940 0.27 +0.6 1,350,927 + BPHYT_RS05940 0.65 +0.8 1,351,254 + +0.3 1,351,254 + -0.4 1,351,311 + BPHYT_RS05945 0.10 +2.1 1,351,311 + BPHYT_RS05945 0.10 +2.4
Or see this region's nucleotide sequence