Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32430

Experiment: 36hr PHYTO11 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32425 and BPHYT_RS32430 are separated by 111 nucleotidesBPHYT_RS32430 and BPHYT_RS32435 overlap by 14 nucleotidesBPHYT_RS32435 and BPHYT_RS32440 are separated by 9 nucleotides BPHYT_RS32425: BPHYT_RS32425 - formate dehydrogenase accessory protein FdhE, at 2,847,856 to 2,848,851 _RS32425 BPHYT_RS32430: BPHYT_RS32430 - formate dehydrogenase-N subunit gamma, at 2,848,963 to 2,849,619 _RS32430 BPHYT_RS32435: BPHYT_RS32435 - formate dehydrogenase-N subunit beta, at 2,849,606 to 2,850,538 _RS32435 BPHYT_RS32440: BPHYT_RS32440 - formate dehydrogenase, at 2,850,548 to 2,852,977 _RS32440 Position (kb) 2848 2849 2850Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2848.035 kb on - strand, within BPHYT_RS32425at 2848.223 kb on + strand, within BPHYT_RS32425at 2848.223 kb on + strand, within BPHYT_RS32425at 2848.224 kb on - strand, within BPHYT_RS32425at 2848.224 kb on - strand, within BPHYT_RS32425at 2848.352 kb on - strand, within BPHYT_RS32425at 2848.517 kb on - strand, within BPHYT_RS32425at 2848.517 kb on - strand, within BPHYT_RS32425at 2848.854 kb on + strandat 2848.951 kb on - strandat 2848.951 kb on - strandat 2849.018 kb on + strandat 2849.018 kb on + strandat 2849.076 kb on - strand, within BPHYT_RS32430at 2849.266 kb on - strand, within BPHYT_RS32430at 2849.348 kb on + strand, within BPHYT_RS32430at 2849.348 kb on + strand, within BPHYT_RS32430at 2849.348 kb on + strand, within BPHYT_RS32430at 2849.349 kb on - strand, within BPHYT_RS32430at 2849.349 kb on - strand, within BPHYT_RS32430at 2849.349 kb on - strand, within BPHYT_RS32430at 2849.470 kb on - strand, within BPHYT_RS32430at 2849.542 kb on - strand, within BPHYT_RS32430at 2849.542 kb on - strand, within BPHYT_RS32430at 2849.642 kb on + strandat 2849.713 kb on - strand, within BPHYT_RS32435at 2850.066 kb on + strand, within BPHYT_RS32435at 2850.067 kb on - strand, within BPHYT_RS32435at 2850.346 kb on - strand, within BPHYT_RS32435at 2850.549 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 0.1
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2,848,035 - BPHYT_RS32425 0.18 -1.2
2,848,223 + BPHYT_RS32425 0.37 -0.7
2,848,223 + BPHYT_RS32425 0.37 +0.5
2,848,224 - BPHYT_RS32425 0.37 +3.5
2,848,224 - BPHYT_RS32425 0.37 -0.9
2,848,352 - BPHYT_RS32425 0.50 -0.2
2,848,517 - BPHYT_RS32425 0.66 -0.5
2,848,517 - BPHYT_RS32425 0.66 +0.3
2,848,854 + +0.7
2,848,951 - -0.1
2,848,951 - -0.4
2,849,018 + +0.0
2,849,018 + -0.5
2,849,076 - BPHYT_RS32430 0.17 +0.2
2,849,266 - BPHYT_RS32430 0.46 -0.4
2,849,348 + BPHYT_RS32430 0.59 -0.6
2,849,348 + BPHYT_RS32430 0.59 +2.5
2,849,348 + BPHYT_RS32430 0.59 -1.2
2,849,349 - BPHYT_RS32430 0.59 +0.6
2,849,349 - BPHYT_RS32430 0.59 +0.1
2,849,349 - BPHYT_RS32430 0.59 -0.1
2,849,470 - BPHYT_RS32430 0.77 -0.1
2,849,542 - BPHYT_RS32430 0.88 +0.5
2,849,542 - BPHYT_RS32430 0.88 +0.6
2,849,642 + -1.9
2,849,713 - BPHYT_RS32435 0.11 +1.4
2,850,066 + BPHYT_RS32435 0.49 +1.5
2,850,067 - BPHYT_RS32435 0.49 -0.1
2,850,346 - BPHYT_RS32435 0.79 -0.8
2,850,549 + +3.8

Or see this region's nucleotide sequence