Experiment: 36hr PHYTO11 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29015 and BPHYT_RS29020 are separated by 113 nucleotides BPHYT_RS29020 and BPHYT_RS29025 are separated by 410 nucleotides BPHYT_RS29025 and BPHYT_RS29030 are separated by 377 nucleotides BPHYT_RS29030 and BPHYT_RS29035 are separated by 69 nucleotides
BPHYT_RS29015: BPHYT_RS29015 - RND transporter, at 2,066,861 to 2,068,432
_RS29015
BPHYT_RS29020: BPHYT_RS29020 - hypothetical protein, at 2,068,546 to 2,068,836
_RS29020
BPHYT_RS29025: BPHYT_RS29025 - hypothetical protein, at 2,069,247 to 2,069,582
_RS29025
BPHYT_RS29030: BPHYT_RS29030 - hypothetical protein, at 2,069,960 to 2,070,205
_RS29030
BPHYT_RS29035: BPHYT_RS29035 - arsenic transporter, at 2,070,275 to 2,071,525
_RS29035
Position (kb)
2069
2070 Strain fitness (log2 ratio)
-2
-1
0
1 at 2068.601 kb on + strand, within BPHYT_RS29020 at 2069.404 kb on - strand, within BPHYT_RS29025 at 2069.642 kb on + strand at 2069.642 kb on + strand at 2069.695 kb on - strand at 2069.753 kb on - strand at 2069.967 kb on - strand at 2070.214 kb on + strand at 2070.225 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 0.1 remove 2,068,601 + BPHYT_RS29020 0.19 +0.5 2,069,404 - BPHYT_RS29025 0.47 -2.4 2,069,642 + -0.7 2,069,642 + +0.8 2,069,695 - -0.4 2,069,753 - +0.9 2,069,967 - -2.7 2,070,214 + -1.4 2,070,225 + +0.1
Or see this region's nucleotide sequence