Experiment: 36hr PHYTO11 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24160 and BPHYT_RS24165 are separated by 51 nucleotides BPHYT_RS24165 and BPHYT_RS24170 overlap by 4 nucleotides BPHYT_RS24170 and BPHYT_RS24175 are separated by 587 nucleotides
BPHYT_RS24160: BPHYT_RS24160 - D-galactarate dehydratase, at 988,328 to 989,917
_RS24160
BPHYT_RS24165: BPHYT_RS24165 - NAD-dependent dehydratase, at 989,969 to 990,811
_RS24165
BPHYT_RS24170: BPHYT_RS24170 - gluconolactonase, at 990,808 to 991,737
_RS24170
BPHYT_RS24175: BPHYT_RS24175 - C4-dicarboxylate ABC transporter, at 992,325 to 993,317
_RS24175
Position (kb) 990
991
992 Strain fitness (log2 ratio) -1
0
1
2 at 989.962 kb on + strand at 989.963 kb on - strand at 989.963 kb on - strand at 989.963 kb on - strand at 990.278 kb on + strand, within BPHYT_RS24165 at 990.449 kb on + strand, within BPHYT_RS24165 at 990.452 kb on + strand, within BPHYT_RS24165 at 990.615 kb on - strand, within BPHYT_RS24165 at 991.342 kb on + strand, within BPHYT_RS24170 at 991.343 kb on - strand, within BPHYT_RS24170 at 991.343 kb on - strand, within BPHYT_RS24170 at 991.994 kb on - strand at 992.040 kb on + strand at 992.330 kb on + strand at 992.331 kb on - strand at 992.446 kb on - strand, within BPHYT_RS24175 at 992.531 kb on + strand, within BPHYT_RS24175
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 0.1 remove 989,962 + +0.4 989,963 - +0.8 989,963 - +1.5 989,963 - -1.2 990,278 + BPHYT_RS24165 0.37 -0.2 990,449 + BPHYT_RS24165 0.57 +0.0 990,452 + BPHYT_RS24165 0.57 +0.2 990,615 - BPHYT_RS24165 0.77 +0.5 991,342 + BPHYT_RS24170 0.57 -0.4 991,343 - BPHYT_RS24170 0.58 -0.5 991,343 - BPHYT_RS24170 0.58 -1.6 991,994 - +2.1 992,040 + +2.3 992,330 + +0.4 992,331 - +0.1 992,446 - BPHYT_RS24175 0.12 -0.5 992,531 + BPHYT_RS24175 0.21 +0.0
Or see this region's nucleotide sequence