Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30905 and BPHYT_RS30910 overlap by 4 nucleotides BPHYT_RS30910 and BPHYT_RS30915 are separated by 197 nucleotides BPHYT_RS30915 and BPHYT_RS30920 are separated by 255 nucleotides
BPHYT_RS30905: BPHYT_RS30905 - histidine kinase, at 2,486,428 to 2,488,089
_RS30905
BPHYT_RS30910: BPHYT_RS30910 - histidine kinase, at 2,488,086 to 2,490,251
_RS30910
BPHYT_RS30915: BPHYT_RS30915 - hypothetical protein, at 2,490,449 to 2,490,790
_RS30915
BPHYT_RS30920: BPHYT_RS30920 - hypothetical protein, at 2,491,046 to 2,491,927
_RS30920
Position (kb)
2488
2489
2490
2491 Strain fitness (log2 ratio)
-1
0
1 at 2487.321 kb on - strand, within BPHYT_RS30905 at 2487.746 kb on + strand, within BPHYT_RS30905 at 2488.148 kb on - strand at 2488.823 kb on + strand, within BPHYT_RS30910 at 2489.713 kb on + strand, within BPHYT_RS30910 at 2489.714 kb on - strand, within BPHYT_RS30910 at 2489.794 kb on + strand, within BPHYT_RS30910 at 2489.795 kb on - strand, within BPHYT_RS30910 at 2490.682 kb on - strand, within BPHYT_RS30915 at 2490.790 kb on + strand at 2490.806 kb on - strand at 2490.806 kb on - strand at 2490.970 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 2,487,321 - BPHYT_RS30905 0.54 -0.8 2,487,746 + BPHYT_RS30905 0.79 -0.7 2,488,148 - -0.0 2,488,823 + BPHYT_RS30910 0.34 +0.8 2,489,713 + BPHYT_RS30910 0.75 +0.2 2,489,714 - BPHYT_RS30910 0.75 +0.7 2,489,794 + BPHYT_RS30910 0.79 +0.6 2,489,795 - BPHYT_RS30910 0.79 -1.7 2,490,682 - BPHYT_RS30915 0.68 +0.3 2,490,790 + -0.6 2,490,806 - +0.7 2,490,806 - +0.2 2,490,970 - -0.4
Or see this region's nucleotide sequence