Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28945

Experiment: 36hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28940 and BPHYT_RS28945 are separated by 10 nucleotidesBPHYT_RS28945 and BPHYT_RS28950 overlap by 10 nucleotides BPHYT_RS28940: BPHYT_RS28940 - divalent ion tolerance protein CutA, at 2,042,739 to 2,045,321 _RS28940 BPHYT_RS28945: BPHYT_RS28945 - endo-1,4-D-glucanase, at 2,045,332 to 2,046,513 _RS28945 BPHYT_RS28950: BPHYT_RS28950 - cellulose synthase, at 2,046,504 to 2,051,213 _RS28950 Position (kb) 2045 2046 2047Strain fitness (log2 ratio) -2 -1 0 1 2at 2044.444 kb on - strand, within BPHYT_RS28940at 2044.444 kb on - strand, within BPHYT_RS28940at 2044.446 kb on + strand, within BPHYT_RS28940at 2044.548 kb on + strand, within BPHYT_RS28940at 2044.549 kb on - strand, within BPHYT_RS28940at 2045.164 kb on - strandat 2045.193 kb on + strandat 2045.193 kb on + strandat 2045.320 kb on - strandat 2045.323 kb on - strandat 2045.708 kb on - strand, within BPHYT_RS28945at 2046.022 kb on - strand, within BPHYT_RS28945at 2046.118 kb on + strand, within BPHYT_RS28945at 2046.783 kb on + strandat 2046.988 kb on - strand, within BPHYT_RS28950at 2047.255 kb on - strand, within BPHYT_RS28950

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1
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2,044,444 - BPHYT_RS28940 0.66 -0.5
2,044,444 - BPHYT_RS28940 0.66 -0.8
2,044,446 + BPHYT_RS28940 0.66 +1.5
2,044,548 + BPHYT_RS28940 0.70 -0.1
2,044,549 - BPHYT_RS28940 0.70 +0.2
2,045,164 - +0.9
2,045,193 + +2.1
2,045,193 + -2.7
2,045,320 - -1.0
2,045,323 - -0.4
2,045,708 - BPHYT_RS28945 0.32 -0.7
2,046,022 - BPHYT_RS28945 0.58 -0.1
2,046,118 + BPHYT_RS28945 0.66 -1.1
2,046,783 + -1.2
2,046,988 - BPHYT_RS28950 0.10 -1.0
2,047,255 - BPHYT_RS28950 0.16 -0.5

Or see this region's nucleotide sequence