Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28135 and BPHYT_RS28140 are separated by 337 nucleotides BPHYT_RS28140 and BPHYT_RS28145 are separated by 57 nucleotides BPHYT_RS28145 and BPHYT_RS28150 are separated by 199 nucleotides
BPHYT_RS28135: BPHYT_RS28135 - alcohol dehydrogenase, at 1,870,175 to 1,871,194
_RS28135
BPHYT_RS28140: BPHYT_RS28140 - translation initiation factor SUI1, at 1,871,532 to 1,871,894
_RS28140
BPHYT_RS28145: BPHYT_RS28145 - diguanylate cyclase, at 1,871,952 to 1,873,133
_RS28145
BPHYT_RS28150: BPHYT_RS28150 - methylmalonate-semialdehyde dehydrogenase, at 1,873,333 to 1,874,850
_RS28150
Position (kb)
1871
1872
1873
1874 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1870.985 kb on - strand, within BPHYT_RS28135 at 1871.362 kb on + strand at 1871.387 kb on + strand at 1871.388 kb on - strand at 1871.388 kb on - strand at 1871.445 kb on - strand at 1871.670 kb on + strand, within BPHYT_RS28140 at 1871.917 kb on + strand at 1871.917 kb on + strand at 1871.918 kb on - strand at 1871.918 kb on - strand at 1872.117 kb on + strand, within BPHYT_RS28145 at 1872.118 kb on - strand, within BPHYT_RS28145 at 1872.391 kb on - strand, within BPHYT_RS28145 at 1872.559 kb on + strand, within BPHYT_RS28145 at 1872.560 kb on - strand, within BPHYT_RS28145 at 1872.560 kb on - strand at 1872.643 kb on + strand, within BPHYT_RS28145 at 1872.781 kb on - strand, within BPHYT_RS28145 at 1873.023 kb on + strand at 1873.024 kb on - strand at 1873.101 kb on - strand at 1873.108 kb on + strand at 1873.109 kb on - strand at 1873.152 kb on - strand at 1873.305 kb on - strand at 1873.406 kb on + strand at 1873.407 kb on - strand at 1873.414 kb on - strand at 1873.581 kb on + strand, within BPHYT_RS28150 at 1874.007 kb on - strand, within BPHYT_RS28150 at 1874.133 kb on - strand, within BPHYT_RS28150
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 1,870,985 - BPHYT_RS28135 0.79 -0.4 1,871,362 + -0.9 1,871,387 + +1.4 1,871,388 - +1.4 1,871,388 - +1.0 1,871,445 - -0.2 1,871,670 + BPHYT_RS28140 0.38 -0.4 1,871,917 + -0.9 1,871,917 + -0.5 1,871,918 - -0.4 1,871,918 - -0.7 1,872,117 + BPHYT_RS28145 0.14 -0.4 1,872,118 - BPHYT_RS28145 0.14 -0.2 1,872,391 - BPHYT_RS28145 0.37 -0.2 1,872,559 + BPHYT_RS28145 0.51 +0.3 1,872,560 - BPHYT_RS28145 0.51 +0.1 1,872,560 - +1.7 1,872,643 + BPHYT_RS28145 0.58 +0.2 1,872,781 - BPHYT_RS28145 0.70 -1.2 1,873,023 + +0.1 1,873,024 - -0.2 1,873,101 - -0.1 1,873,108 + +1.6 1,873,109 - -0.5 1,873,152 - -0.2 1,873,305 - -0.8 1,873,406 + +3.4 1,873,407 - -0.3 1,873,414 - +2.0 1,873,581 + BPHYT_RS28150 0.16 +0.3 1,874,007 - BPHYT_RS28150 0.44 +0.1 1,874,133 - BPHYT_RS28150 0.53 +1.2
Or see this region's nucleotide sequence