Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25515

Experiment: 36hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25500 and BPHYT_RS25505 overlap by 4 nucleotidesBPHYT_RS25505 and BPHYT_RS25510 are separated by 16 nucleotidesBPHYT_RS25510 and BPHYT_RS25515 are separated by 83 nucleotidesBPHYT_RS25515 and BPHYT_RS25520 are separated by 172 nucleotides BPHYT_RS25500: BPHYT_RS25500 - hypothetical protein, at 1,297,166 to 1,297,807 _RS25500 BPHYT_RS25505: BPHYT_RS25505 - RNA polymerase sigma factor, at 1,297,804 to 1,298,349 _RS25505 BPHYT_RS25510: BPHYT_RS25510 - hypothetical protein, at 1,298,366 to 1,298,686 _RS25510 BPHYT_RS25515: BPHYT_RS25515 - glutathione S-transferase, at 1,298,770 to 1,299,471 _RS25515 BPHYT_RS25520: BPHYT_RS25520 - TetR family transcriptional regulator, at 1,299,644 to 1,300,237 _RS25520 Position (kb) 1298 1299 1300Strain fitness (log2 ratio) -1 0 1at 1298.100 kb on + strand, within BPHYT_RS25505at 1298.100 kb on + strand, within BPHYT_RS25505at 1299.243 kb on - strand, within BPHYT_RS25515at 1299.710 kb on + strand, within BPHYT_RS25520at 1299.717 kb on - strand, within BPHYT_RS25520at 1299.718 kb on + strand, within BPHYT_RS25520at 1299.724 kb on + strand, within BPHYT_RS25520at 1300.370 kb on + strandat 1300.371 kb on - strandat 1300.460 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1
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1,298,100 + BPHYT_RS25505 0.54 -0.5
1,298,100 + BPHYT_RS25505 0.54 -0.8
1,299,243 - BPHYT_RS25515 0.67 -1.4
1,299,710 + BPHYT_RS25520 0.11 -0.2
1,299,717 - BPHYT_RS25520 0.12 +1.1
1,299,718 + BPHYT_RS25520 0.12 +0.3
1,299,724 + BPHYT_RS25520 0.13 -0.1
1,300,370 + -0.5
1,300,371 - +1.5
1,300,460 - +0.3

Or see this region's nucleotide sequence