Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23315 and BPHYT_RS23320 are separated by 123 nucleotides BPHYT_RS23320 and BPHYT_RS23325 are separated by 8 nucleotides BPHYT_RS23325 and BPHYT_RS23330 are separated by 400 nucleotides BPHYT_RS23330 and BPHYT_RS23335 are separated by 8 nucleotides
BPHYT_RS23315: BPHYT_RS23315 - hypothetical protein, at 792,641 to 793,042
_RS23315
BPHYT_RS23320: BPHYT_RS23320 - hypothetical protein, at 793,166 to 793,630
_RS23320
BPHYT_RS23325: BPHYT_RS23325 - ribonuclease, at 793,639 to 794,094
_RS23325
BPHYT_RS23330: BPHYT_RS23330 - hypothetical protein, at 794,495 to 794,680
_RS23330
BPHYT_RS23335: BPHYT_RS23335 - aspartate racemase, at 794,689 to 795,390
_RS23335
Position (kb) 793
794
795 Strain fitness (log2 ratio) -1
0
1 at 792.897 kb on - strand, within BPHYT_RS23315 at 793.137 kb on + strand at 793.335 kb on + strand, within BPHYT_RS23320 at 793.435 kb on + strand, within BPHYT_RS23320 at 793.470 kb on - strand, within BPHYT_RS23320 at 793.477 kb on - strand, within BPHYT_RS23320 at 793.481 kb on + strand, within BPHYT_RS23320 at 793.617 kb on + strand at 793.641 kb on - strand at 793.683 kb on - strand at 793.721 kb on - strand, within BPHYT_RS23325 at 793.721 kb on - strand, within BPHYT_RS23325 at 793.769 kb on + strand, within BPHYT_RS23325 at 793.770 kb on - strand, within BPHYT_RS23325 at 794.138 kb on - strand at 794.138 kb on - strand at 794.144 kb on + strand at 794.387 kb on + strand at 794.405 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 792,897 - BPHYT_RS23315 0.64 -0.9 793,137 + -0.3 793,335 + BPHYT_RS23320 0.36 +0.6 793,435 + BPHYT_RS23320 0.58 +0.6 793,470 - BPHYT_RS23320 0.65 -0.6 793,477 - BPHYT_RS23320 0.67 +0.1 793,481 + BPHYT_RS23320 0.68 +1.2 793,617 + -0.5 793,641 - +0.2 793,683 - -1.3 793,721 - BPHYT_RS23325 0.18 +0.2 793,721 - BPHYT_RS23325 0.18 -0.8 793,769 + BPHYT_RS23325 0.29 -0.1 793,770 - BPHYT_RS23325 0.29 -0.5 794,138 - +0.4 794,138 - -0.6 794,144 + +0.6 794,387 + +0.8 794,405 + -0.4
Or see this region's nucleotide sequence