Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23095 and BPHYT_RS23100 are separated by 26 nucleotides BPHYT_RS23100 and BPHYT_RS23105 are separated by 81 nucleotides
BPHYT_RS23095: BPHYT_RS23095 - MFS transporter, at 745,127 to 746,359
_RS23095
BPHYT_RS23100: BPHYT_RS23100 - sugar ABC transporter substrate-binding protein, at 746,386 to 747,333
_RS23100
BPHYT_RS23105: BPHYT_RS23105 - DNA polymerase, at 747,415 to 749,790
_RS23105
Position (kb) 746
747
748 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 745.508 kb on + strand, within BPHYT_RS23095 at 745.634 kb on + strand, within BPHYT_RS23095 at 745.868 kb on + strand, within BPHYT_RS23095 at 745.929 kb on - strand, within BPHYT_RS23095 at 746.186 kb on - strand, within BPHYT_RS23095 at 746.330 kb on + strand at 746.387 kb on + strand at 746.388 kb on - strand at 746.388 kb on - strand at 746.512 kb on + strand, within BPHYT_RS23100 at 746.513 kb on - strand, within BPHYT_RS23100 at 747.406 kb on - strand at 747.578 kb on + strand at 747.579 kb on - strand at 747.579 kb on - strand at 747.874 kb on - strand, within BPHYT_RS23105 at 747.876 kb on - strand, within BPHYT_RS23105 at 748.057 kb on - strand, within BPHYT_RS23105 at 748.145 kb on + strand, within BPHYT_RS23105 at 748.146 kb on - strand, within BPHYT_RS23105 at 748.147 kb on + strand, within BPHYT_RS23105 at 748.320 kb on + strand, within BPHYT_RS23105 at 748.321 kb on - strand, within BPHYT_RS23105
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 745,508 + BPHYT_RS23095 0.31 +1.1 745,634 + BPHYT_RS23095 0.41 +0.9 745,868 + BPHYT_RS23095 0.60 +0.2 745,929 - BPHYT_RS23095 0.65 -0.4 746,186 - BPHYT_RS23095 0.86 -0.4 746,330 + -2.0 746,387 + -1.4 746,388 - +0.0 746,388 - -0.6 746,512 + BPHYT_RS23100 0.13 +0.2 746,513 - BPHYT_RS23100 0.13 +0.3 747,406 - +0.1 747,578 + -0.1 747,579 - +0.5 747,579 - -1.6 747,874 - BPHYT_RS23105 0.19 +1.6 747,876 - BPHYT_RS23105 0.19 -0.7 748,057 - BPHYT_RS23105 0.27 -2.2 748,145 + BPHYT_RS23105 0.31 -0.5 748,146 - BPHYT_RS23105 0.31 -1.4 748,147 + BPHYT_RS23105 0.31 -3.0 748,320 + BPHYT_RS23105 0.38 -0.3 748,321 - BPHYT_RS23105 0.38 -1.9
Or see this region's nucleotide sequence