Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22400

Experiment: 36hr PHYTO11 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22395 and BPHYT_RS22400 are separated by 181 nucleotidesBPHYT_RS22400 and BPHYT_RS22405 are separated by 57 nucleotidesBPHYT_RS22405 and BPHYT_RS22410 are separated by 70 nucleotidesBPHYT_RS22410 and BPHYT_RS22415 overlap by 4 nucleotides BPHYT_RS22395: BPHYT_RS22395 - AraC family transcriptional regulator, at 580,497 to 581,450 _RS22395 BPHYT_RS22400: BPHYT_RS22400 - hypothetical protein, at 581,632 to 581,958 _RS22400 BPHYT_RS22405: BPHYT_RS22405 - HxlR family transcriptional regulator, at 582,016 to 582,417 _RS22405 BPHYT_RS22410: BPHYT_RS22410 - hypothetical protein, at 582,488 to 582,913 _RS22410 BPHYT_RS22415: BPHYT_RS22415 - nitrite reductase, at 582,910 to 584,700 _RS22415 Position (kb) 581 582Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 580.743 kb on + strand, within BPHYT_RS22395at 580.743 kb on + strand, within BPHYT_RS22395at 580.958 kb on + strand, within BPHYT_RS22395at 581.275 kb on - strand, within BPHYT_RS22395at 581.547 kb on - strandat 581.548 kb on + strandat 581.552 kb on + strandat 581.553 kb on - strandat 581.553 kb on - strandat 581.562 kb on + strandat 581.794 kb on + strand, within BPHYT_RS22400at 581.926 kb on + strandat 581.944 kb on - strandat 581.975 kb on + strandat 581.975 kb on + strandat 581.976 kb on - strandat 581.979 kb on - strandat 582.006 kb on - strandat 582.017 kb on + strandat 582.417 kb on + strandat 582.538 kb on + strand, within BPHYT_RS22410at 582.539 kb on - strand, within BPHYT_RS22410at 582.539 kb on - strand, within BPHYT_RS22410at 582.552 kb on + strand, within BPHYT_RS22410at 582.721 kb on - strand, within BPHYT_RS22410at 582.733 kb on - strand, within BPHYT_RS22410

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1
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580,743 + BPHYT_RS22395 0.26 -0.7
580,743 + BPHYT_RS22395 0.26 -0.2
580,958 + BPHYT_RS22395 0.48 +0.5
581,275 - BPHYT_RS22395 0.82 +0.2
581,547 - -0.5
581,548 + -2.3
581,552 + +1.1
581,553 - +0.2
581,553 - -1.8
581,562 + +0.3
581,794 + BPHYT_RS22400 0.50 -1.3
581,926 + -3.1
581,944 - +2.1
581,975 + +0.4
581,975 + -0.4
581,976 - -1.2
581,979 - +0.2
582,006 - +1.0
582,017 + -0.3
582,417 + +1.1
582,538 + BPHYT_RS22410 0.12 -0.1
582,539 - BPHYT_RS22410 0.12 -0.5
582,539 - BPHYT_RS22410 0.12 -0.7
582,552 + BPHYT_RS22410 0.15 -1.9
582,721 - BPHYT_RS22410 0.55 -0.4
582,733 - BPHYT_RS22410 0.58 -2.5

Or see this region's nucleotide sequence