Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21990 and BPHYT_RS21995 overlap by 8 nucleotides BPHYT_RS21995 and BPHYT_RS22000 overlap by 4 nucleotides BPHYT_RS22000 and BPHYT_RS22005 are separated by 23 nucleotides
BPHYT_RS21990: BPHYT_RS21990 - FAD-dependent oxidoreductase, at 489,193 to 490,320
_RS21990
BPHYT_RS21995: BPHYT_RS21995 - (2Fe-2S)-binding protein, at 490,313 to 491,746
_RS21995
BPHYT_RS22000: BPHYT_RS22000 - hypothetical protein, at 491,743 to 492,105
_RS22000
BPHYT_RS22005: BPHYT_RS22005 - ABC transporter permease, at 492,129 to 492,923
_RS22005
Position (kb) 490
491
492 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 489.363 kb on + strand at 489.423 kb on + strand, within BPHYT_RS21990 at 489.593 kb on + strand, within BPHYT_RS21990 at 489.653 kb on + strand, within BPHYT_RS21990 at 489.686 kb on + strand, within BPHYT_RS21990 at 490.571 kb on - strand, within BPHYT_RS21995 at 491.119 kb on - strand, within BPHYT_RS21995 at 491.202 kb on - strand, within BPHYT_RS21995 at 491.232 kb on + strand, within BPHYT_RS21995 at 491.252 kb on + strand, within BPHYT_RS21995 at 491.496 kb on + strand, within BPHYT_RS21995 at 491.788 kb on + strand, within BPHYT_RS22000 at 491.982 kb on + strand, within BPHYT_RS22000 at 492.164 kb on - strand at 492.256 kb on + strand, within BPHYT_RS22005 at 492.256 kb on + strand, within BPHYT_RS22005 at 492.256 kb on + strand, within BPHYT_RS22005 at 492.256 kb on + strand, within BPHYT_RS22005 at 492.262 kb on + strand, within BPHYT_RS22005 at 492.263 kb on - strand, within BPHYT_RS22005 at 492.588 kb on + strand, within BPHYT_RS22005
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 489,363 + +4.1 489,423 + BPHYT_RS21990 0.20 +0.7 489,593 + BPHYT_RS21990 0.35 +2.9 489,653 + BPHYT_RS21990 0.41 -1.6 489,686 + BPHYT_RS21990 0.44 +0.5 490,571 - BPHYT_RS21995 0.18 +2.1 491,119 - BPHYT_RS21995 0.56 +0.5 491,202 - BPHYT_RS21995 0.62 +1.5 491,232 + BPHYT_RS21995 0.64 +1.0 491,252 + BPHYT_RS21995 0.65 +0.3 491,496 + BPHYT_RS21995 0.82 -0.8 491,788 + BPHYT_RS22000 0.12 -1.2 491,982 + BPHYT_RS22000 0.66 +0.2 492,164 - +0.2 492,256 + BPHYT_RS22005 0.16 +1.2 492,256 + BPHYT_RS22005 0.16 -0.4 492,256 + BPHYT_RS22005 0.16 -2.2 492,256 + BPHYT_RS22005 0.16 -0.1 492,262 + BPHYT_RS22005 0.17 +0.9 492,263 - BPHYT_RS22005 0.17 +0.4 492,588 + BPHYT_RS22005 0.58 -1.3
Or see this region's nucleotide sequence