Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21465 and BPHYT_RS21470 are separated by 385 nucleotides BPHYT_RS21470 and BPHYT_RS21475 are separated by 52 nucleotides BPHYT_RS21475 and BPHYT_RS21480 overlap by 25 nucleotides
BPHYT_RS21465: BPHYT_RS21465 - TetR family transcriptional regulator, at 375,588 to 376,316
_RS21465
BPHYT_RS21470: BPHYT_RS21470 - 6-hydroxynicotinate reductase, at 376,702 to 378,306
_RS21470
BPHYT_RS21475: BPHYT_RS21475 - hypothetical protein, at 378,359 to 379,297
_RS21475
BPHYT_RS21480: BPHYT_RS21480 - hypothetical protein, at 379,273 to 379,863
_RS21480
Position (kb) 376
377
378
379 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 375.763 kb on + strand, within BPHYT_RS21465 at 375.784 kb on + strand, within BPHYT_RS21465 at 375.992 kb on - strand, within BPHYT_RS21465 at 376.087 kb on + strand, within BPHYT_RS21465 at 376.141 kb on + strand, within BPHYT_RS21465 at 376.178 kb on + strand, within BPHYT_RS21465 at 376.201 kb on + strand, within BPHYT_RS21465 at 376.449 kb on - strand at 376.465 kb on - strand at 376.571 kb on - strand at 376.714 kb on - strand at 376.859 kb on + strand at 377.070 kb on + strand, within BPHYT_RS21470 at 377.164 kb on + strand, within BPHYT_RS21470 at 378.022 kb on + strand, within BPHYT_RS21470 at 378.219 kb on + strand at 378.246 kb on + strand at 378.598 kb on + strand, within BPHYT_RS21475 at 378.599 kb on - strand, within BPHYT_RS21475 at 378.599 kb on - strand, within BPHYT_RS21475 at 378.632 kb on + strand, within BPHYT_RS21475 at 378.633 kb on - strand, within BPHYT_RS21475 at 378.746 kb on + strand, within BPHYT_RS21475
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 375,763 + BPHYT_RS21465 0.24 +0.3 375,784 + BPHYT_RS21465 0.27 -0.8 375,992 - BPHYT_RS21465 0.55 -1.0 376,087 + BPHYT_RS21465 0.68 -0.3 376,141 + BPHYT_RS21465 0.76 +2.0 376,178 + BPHYT_RS21465 0.81 +2.3 376,201 + BPHYT_RS21465 0.84 -0.9 376,449 - -1.5 376,465 - -0.1 376,571 - +0.7 376,714 - +0.7 376,859 + +0.0 377,070 + BPHYT_RS21470 0.23 -0.7 377,164 + BPHYT_RS21470 0.29 -1.2 378,022 + BPHYT_RS21470 0.82 -1.5 378,219 + -0.1 378,246 + +1.9 378,598 + BPHYT_RS21475 0.25 +0.9 378,599 - BPHYT_RS21475 0.26 -2.0 378,599 - BPHYT_RS21475 0.26 +0.2 378,632 + BPHYT_RS21475 0.29 -0.4 378,633 - BPHYT_RS21475 0.29 -1.1 378,746 + BPHYT_RS21475 0.41 +5.2
Or see this region's nucleotide sequence