Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20525 and BPHYT_RS20530 are separated by 128 nucleotides BPHYT_RS20530 and BPHYT_RS20535 are separated by 148 nucleotides BPHYT_RS20535 and BPHYT_RS20540 overlap by 19 nucleotides BPHYT_RS20540 and BPHYT_RS20545 are separated by 87 nucleotides
BPHYT_RS20525: BPHYT_RS20525 - esterase, at 166,985 to 168,571
_RS20525
BPHYT_RS20530: BPHYT_RS20530 - PA-phosphatase, at 168,700 to 169,302
_RS20530
BPHYT_RS20535: BPHYT_RS20535 - allantoate amidohydrolase, at 169,451 to 170,692
_RS20535
BPHYT_RS20540: BPHYT_RS20540 - N-acetyltransferase GCN5, at 170,674 to 171,555
_RS20540
BPHYT_RS20545: BPHYT_RS20545 - hypothetical protein, at 171,643 to 171,948
_RS20545
Position (kb) 169
170
171 Strain fitness (log2 ratio) -2
-1
0
1
2 at 168.603 kb on + strand at 168.604 kb on - strand at 168.643 kb on + strand at 169.026 kb on + strand, within BPHYT_RS20530 at 169.027 kb on - strand, within BPHYT_RS20530 at 169.191 kb on - strand, within BPHYT_RS20530 at 169.194 kb on + strand, within BPHYT_RS20530 at 169.195 kb on - strand, within BPHYT_RS20530 at 169.199 kb on + strand, within BPHYT_RS20530 at 169.200 kb on - strand, within BPHYT_RS20530 at 169.200 kb on - strand, within BPHYT_RS20530 at 169.200 kb on - strand, within BPHYT_RS20530 at 169.302 kb on + strand at 169.302 kb on + strand at 169.387 kb on - strand at 169.395 kb on + strand at 169.395 kb on + strand at 169.497 kb on + strand at 169.681 kb on + strand, within BPHYT_RS20535 at 170.285 kb on + strand, within BPHYT_RS20535 at 170.285 kb on + strand, within BPHYT_RS20535 at 170.286 kb on - strand, within BPHYT_RS20535 at 170.286 kb on - strand, within BPHYT_RS20535 at 170.286 kb on - strand, within BPHYT_RS20535 at 170.286 kb on - strand, within BPHYT_RS20535 at 170.602 kb on + strand at 170.808 kb on + strand, within BPHYT_RS20540 at 170.911 kb on + strand, within BPHYT_RS20540 at 170.912 kb on - strand, within BPHYT_RS20540 at 170.912 kb on - strand, within BPHYT_RS20540 at 170.912 kb on - strand, within BPHYT_RS20540 at 170.914 kb on - strand, within BPHYT_RS20540 at 170.977 kb on + strand, within BPHYT_RS20540 at 171.031 kb on + strand, within BPHYT_RS20540 at 171.032 kb on - strand, within BPHYT_RS20540 at 171.112 kb on + strand, within BPHYT_RS20540 at 171.488 kb on - strand at 171.509 kb on + strand at 171.644 kb on + strand at 171.645 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 168,603 + +0.4 168,604 - +0.2 168,643 + +0.5 169,026 + BPHYT_RS20530 0.54 +0.0 169,027 - BPHYT_RS20530 0.54 -2.1 169,191 - BPHYT_RS20530 0.81 -0.9 169,194 + BPHYT_RS20530 0.82 -0.7 169,195 - BPHYT_RS20530 0.82 -0.8 169,199 + BPHYT_RS20530 0.83 +0.9 169,200 - BPHYT_RS20530 0.83 +2.5 169,200 - BPHYT_RS20530 0.83 -0.8 169,200 - BPHYT_RS20530 0.83 +0.2 169,302 + +0.9 169,302 + -0.3 169,387 - +1.3 169,395 + -0.1 169,395 + -1.1 169,497 + -0.4 169,681 + BPHYT_RS20535 0.19 -0.1 170,285 + BPHYT_RS20535 0.67 -0.7 170,285 + BPHYT_RS20535 0.67 -0.2 170,286 - BPHYT_RS20535 0.67 -0.9 170,286 - BPHYT_RS20535 0.67 -0.4 170,286 - BPHYT_RS20535 0.67 +0.1 170,286 - BPHYT_RS20535 0.67 -0.8 170,602 + -0.4 170,808 + BPHYT_RS20540 0.15 -0.0 170,911 + BPHYT_RS20540 0.27 -0.8 170,912 - BPHYT_RS20540 0.27 -1.2 170,912 - BPHYT_RS20540 0.27 +0.2 170,912 - BPHYT_RS20540 0.27 -2.2 170,914 - BPHYT_RS20540 0.27 -1.0 170,977 + BPHYT_RS20540 0.34 -1.0 171,031 + BPHYT_RS20540 0.40 -0.9 171,032 - BPHYT_RS20540 0.41 +0.2 171,112 + BPHYT_RS20540 0.50 +0.2 171,488 - +1.2 171,509 + -0.0 171,644 + -1.5 171,645 - -0.6
Or see this region's nucleotide sequence